A5011774204- 3D Printing in Biomedical Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Pluripotent Stem Cells Research
- Nanoplatforms for cancer theranostics
- Graphene and Nanomaterials Applications
- Nanoparticle-Based Drug Delivery
- Neuroscience and Neural Engineering
- Biosensors and Analytical Detection
- Electrospun Nanofibers in Biomedical Applications
- Hydrogels: synthesis, properties, applications
- Advanced Sensor and Energy Harvesting Materials
- Cell Image Analysis Techniques
- Tissue Engineering and Regenerative Medicine
- Gold and Silver Nanoparticles Synthesis and Applications
- Additive Manufacturing and 3D Printing Technologies
- Bone Tissue Engineering Materials
- Nanofabrication and Lithography Techniques
- Cancer Cells and Metastasis
- Supercapacitor Materials and Fabrication
- Advancements in Transdermal Drug Delivery
- SARS-CoV-2 detection and testing
- Neurogenesis and neuroplasticity mechanisms
- Cellular Mechanics and Interactions
Sogang University
2016-2025
Government of the Republic of Korea
2019-2020
Brigham and Women's Hospital
2004-2012
Harvard University
2007-2012
Hanyang University
1999-2012
Massachusetts Institute of Technology
2007-2010
Harvard–MIT Division of Health Sciences and Technology
2007-2010
University of California, Irvine
2005-2007
Pohang University of Science and Technology
2007
This paper describes a gradient-generating microfluidic platform for optimizing proliferation and differentiation of neural stem cells (NSCs) in culture. Microfluidic technology has great potential to improve cell (SC) cultures, whose promise cell–based therapies is limited by the inability precisely control their behavior Compared traditional culture tools, platforms should provide much greater over microenvironment rapid optimization media composition using relatively small numbers cells....
Recently, various approaches for controlling the embryonic stem (ES) cell microenvironment have been developed regulating cellular fate decisions. It has reported that lineage specific differentiation could be affected by size of ES colonies and embryoid bodies (EBs). However, much underlying biology not well elucidated. In this study, we used microengineered hydrogel microwells to direct determined role WNT signaling pathway in directing differentiation. This was accomplished forming...
Encapsulating cells within hydrogels is important for generating three-dimensional (3D) tissue constructs drug delivery and engineering. This paper describes, the first time, fabrication of large numbers cell-laden microgel particles using a continuous microfluidic process called stop-flow lithography (SFL). Prepolymer solution containing was flowed through device arrays individual were repeatedly defined pulses UV light transparency mask. Unlike photolithography, SFL can be used to...
Abstract A new perspective suggests that a dynamic bidirectional communication system, often referred to as the microbiome-gut-brain axis, exists among gut, its microbiome, and central nervous system (CNS). This may influence brain health various brain-related diseases, especially in realms of neurodevelopmental neurodegenerative conditions. However, exact mechanism is not yet understood. Metabolites or extracellular vesicles derived from microbes gut have capacity traverse intestinal...
We developed microfluidic-based pure chitosan microfibers (∼1 meter long, 70–150 μm diameter) for liver tissue engineering applications. Despite the potential of creating bio-artificial chips, its major limitation is inability to fabricate chitosan-based microstructures with controlled shapes because mechanical weakness chitosan. Previous studies have shown that micro/nanofibers can be fabricated by using chemicals and electrospinning techniques. However, there no paper regarding in a...
Nanoscale drug carriers play an important role in regulating the delivery, permeability, and retention of drugs. Although various have been used to encapsulate anticancer drugs, natural biomaterials are great benefit for delivery controlled release We electrospray deposition system synthesize gliadin gliadin-gelatin composite nanoparticles (e.g., cyclophosphamide). The size profile synthesis was characterized by dynamic light scattering X-ray diffractometry. Cyclophosphamide gradually...
The protein-functionalized reduced graphene oxide (rGO) nanosheet is of great interest in stimuli-responsive drug delivery and controlled release applications. We developed doxorubicin (DOX)-loaded bovine serum albumin (BSA)-functionalized rGO (DOX-BSA-rGO) nanosheets. To investigate the reduction BSA-functionalized GO nanosheets loading efficiency, we used X-ray photoelectron spectroscopy (XPS) UV–visible spectrophotometer analysis. DOX-BSA-rGO exhibited dose-dependent cellular uptake...
Even though in vitro co-culture tumor spheroid model plays an important role screening drug candidates, its wide applications are currently limited due to the lack of reliable and high throughput methods for generating well-defined 3D complex structures. Herein, we report development a hydrogel microwell array generate uniform-sized multicellular spheroids. Our developed spheroids structurally well-defined, robust can be easily transferred into widely used 2D culture substrates while...
Poly-N-isopropyl acrylamide (PNIPAM) nanogels have been modified with different acrylic acid (AAc) contents for the efficient control of lower critical solution temperature (LCST). In this study, PNIPAM-co-AAc showed two volume phase transitions in comparison PNIPAM. The transition PNIPAM was increased AAc contents. controlled drug release performance loaded β-lapachone attributed to content ratio and efficiently triggered response pH. Moreover, a colorimetric cell proliferation assay direct...
Nanoparticle-based nucleic acid conjugates (NP-NACs) hold great promise for theragnostic (diagnostic and therapeutic) applications. However, several limitations have hindered the realization of their full potential in clinical treatment cancer other diseases. In diagnosis, NP-NACs, combined with conventional optical sensing systems, been applied detection
The intestinal microbiome affects a number of biological functions the organism. Although animal model is powerful tool to study relationship between host and microbe, physiologically relevant in vitro human system has still unmet needs. Thus, establishment an living cell-based intestine that can mimic mechanical, structural, absorptive, transport pathophysiological properties environment along with its commensal bacterial strains promote pharmaceutical development potentially replace...
Many chemical and biological processes are dependent on molecular gradients. We describe a new microfluidic approach that can be used to produce spatiotemporal gradients across two-dimensional surfaces three-dimensional gels under flow-free conditions. Free diffusion between dynamically replenished flow channels acting as sink source is utilized give rise stable steady-state gradient profiles. The profile dictated by the engineered design of device's gradient-generating region. Different...
In this article, we describe an approach to generate microporous cell-laden hydrogels for fabricating biomimetic tissue engineered constructs. Micropores at different length scales were fabricated in by micromolding fluidic channels and leaching sucrose crystals. Microengineered created within hydrogel precursors containing agarose solution mixed with The rapid cooling of the was used gel form micropores place process generated homogeneously distributed gels, while enabling direct...