Many underlying causes of human infertility have been overcome by using in vitro fertilization (IVF) and embryo transfer (ET) techniques. Nevertheless, implantation rates IVF programs remain low despite the apparently healthy embryos. This suggests that there are problems with differentiation uterus to receptive state response ovarian hormones estrogen progesterone. The molecular basis this when uterine environment is conducive blastocyst acceptance remains poorly understood. Normally,...

Cytosolic phospholipase A2α (cPLA2α) is a major provider of arachidonic acid (AA) for the cyclooxygenase (COX) system biosynthesis prostaglandins (PGs). Female mice with null mutation Pla2g4a produce small litters and often exhibit pregnancy failures, although cause(s) these defects remains elusive. We show that initiation implantation temporarily deferred in Pla2g4a–/– mice, shifting normal ‘window’ leading to retarded feto-placental development without apparent decidual growth....

Abstract Various mediators, including cytokines, growth factors, homeotic gene products, and prostaglandins (PGs), participate in the implantation process an autocrine, paracrine, or juxtacrine manner. However, interactions among these factors that result successful are not clearly understood. Leukemia inhibitory factor (LIF), a pleiotropic cytokine, was shown to be expressed uterine glands on day 4 morning before is critical this mice. mechanism by which LIF executes its effects remains...

Infertility and spontaneous pregnancy losses are an enduring problem to women's health. The establishment of depends on successful implantation, where a complex series interactions occurs between the heterogeneous cell types uterus blastocyst. Although number genes implicated in embryo-uterine during genetic evidence suggests that only small them critical this process. To obtain global view identify novel pathways we used dual screening strategy analyze expression nearly 10,000 mouse by...

The mechanisms by which synchronized embryonic development to the blastocyst stage, preparation of uterus for receptive state, and reciprocal embryo-uterine interactions implantation are coordinated still unclear. We show in this study that preimplantation embryo became asynchronous mice deficient brain-type (CB1) and/or spleen-type (CB2) cannabinoid receptor genes. Furthermore, whereas levels uterine anandamide (endocannabinoid) CB1 coordinately down-regulated with onset receptivity...

Intimate two-way interactions between the implantation-competent blastocyst and receptive uterus are prerequisite for successful embryo implantation. In humans, recurrent/repeated implantation failure (RIF) may occur due to altered uterine receptivity with aberrant gene expression in endometrium as well genetic defects embryos. Several studies have been performed understand dynamic changes of transcriptome during menstrual cycles humans. However, patients RIF has not clearly investigated...

Abstract Successful implantation absolutely depends on the reciprocal interaction between implantation-competent blastocyst and receptive uterus. Expression gene targeting studies have shown that leukemia inhibitory factor (LIF), a cytokine of IL-6 family, Hoxa-10, an abdominalB-like homeobox gene, are crucial to decidualization in mice. Using these mutant mice, we sought determine importance Msx-1 (another formerly known as Hox-7.1) Wnt4 (a ligand Wnt family) signaling because their...

PDS5B is a sister chromatid cohesion protein that crucial for faithful segregation of duplicated chromosomes in lower organisms. Mutations proteins are associated with the developmental disorder Cornelia de Lange syndrome (CdLS) humans. To delineate physiological roles mammals, we generated mice lacking (APRIN). Pds5B-deficient died shortly after birth. They exhibited multiple congenital anomalies,including heart defects, cleft palate, fusion ribs, short limbs, distal colon aganglionosis,...

Delayed implantation, considered a state of suspended animation, is widespread in mammals. Blastocysts under this condition remain dormant for an extended period but resume implantation competence upon favorable conditions. The underlying mechanism by which longevity blastocysts maintained not clearly understood. Using autophagy markers and the well-defined delayed model mice, we show that important utero during implantation. However, prolonged dormancy leads to reduced developmental...

Abstract Background Clinical use of mesenchymal stem cells (MSCs) requires a uniform cell population, and their harvesting is invasive produces limited number cells. Human embryonic cell-derived MSCs (hESC-MSCs) can differentiate into three germ layers possess immunosuppressive effects in vitro. Anticancer treatment well-known risk factor for premature ovarian failure (POF). In this study, we investigated the effect hESC-MSC on recovery function cisplatin-induced POF mice. Methods Female...

Asherman syndrome (AS) is characterized by intrauterine adhesion or fibrosis resulting from damage to the endometrium, often leading amenorrhea, infertility, recurrent pregnancy loss. Although various therapeutic strategies for AS have been proposed, options remain limited. New such as bone marrow-derived mesenchymal stem cell (BM-MSC) therapy aim potentiate intrinsic capacity of endometrial regeneration. However, BM-MSC has not widely adopted mainly because it involves invasive and...

Objectives To compare gene expression profiles of placentas from preeclamptic and normal pregnancies. Study design We performed microarray experiments to analyze genome-wide profiling for 10 pregnant women with preeclampsia who experienced noncomplicated pregnancies (CON), identify dysregulated signaling pathways as well genes in preeclampsia. RT-PCR, real-time RT-PCR and/or immunofluorescence analyses were validate the data obtained experiments. Results Unsupervised hierarchical clustering...

Autophagy is a major cellular catabolic pathway tightly associated with cell survival. The involvement of autophagy in the prolonged survival blastocysts uterus well established, and it was assumed that ovarian steroid hormones - progesterone (P4) estrogens have important roles regulation autophagy. However, information scarce regarding whether these regulate certain hormone-responsive systems. In this study, we investigated effects estrogen P4 on autophagic response uteri pregnant mice...

Polycystic ovary syndrome (PCOS) is a common endocrine disorder characterized by chronic oligo/anovulation, hyperandrogenemia, infertility, and metabolic alterations related to insulin resistance. These abnormalities in PCOS may have complex effects on pathophysiology of the endometrium, contributing infertility endometrial disorders.The objective this study was examine dysregulated signaling pathways endometrium patients with (PCOSE) analyzing expression profiles pathway-oriented...

Vitrification involves the use of cryoprotectants (CPAs) and liquid nitrogen (LN2), which may cause osmotic damage cryoinjury to oocytes. Autophagy is widely recognized as a survival or response mechanism elicited by various environmental cellular stressors. However, induction autophagy in vitrified-warmed oocytes has not been examined. In this work, we investigated whether vitrification-warming process induces mouse Metaphase II (MII) that were vitrified stored LN2 for at least 2 weeks used...

Cadmium (Cd), a major component of cigarette smoke, disrupts the normal functions airway cells and can lead to development various pulmonary diseases such as chronic obstructive disease (COPD). However, molecular mechanisms involved in Cd-induced are poorly understood. Here, we identified cluster genes that altered response Cd exposure human bronchial epithelial (BEAS-2B) demonstrated ER stress inflammation mediated via CCAAT-enhancer-binding proteins (C/EBP)-DNA-damaged-inducible transcript...

Abstract Problem Maternal inflammation leads to preterm birth and perinatal brain injury. Melatonin, through its anti‐inflammatory effects, has been shown be protective against inflammation‐induced adverse effects. However, the immunomodulatory effects of melatonin on prematurity‐related morbidity remain unknown. We wanted investigate maternally administered injury in a mouse model maternal inflammation. Method study A employing lipopolysaccharide (LPS) was used mimic most common clinical...