A5086556319- CRISPR and Genetic Engineering
- RNA regulation and disease
- RNA Interference and Gene Delivery
- Hearing, Cochlea, Tinnitus, Genetics
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Plant Molecular Biology Research
- Plant Virus Research Studies
- Virus-based gene therapy research
- Hearing Loss and Rehabilitation
- Plant and Fungal Interactions Research
- Cocoa and Sweet Potato Agronomy
- AI in cancer detection
- Visual perception and processing mechanisms
- COVID-19 diagnosis using AI
- RNA modifications and cancer
- Advances in Cucurbitaceae Research
- Microbial Community Ecology and Physiology
- Bacillus and Francisella bacterial research
- Pancreatic and Hepatic Oncology Research
- Medical Research and Treatments
- Hepatocellular Carcinoma Treatment and Prognosis
- Magnetic and Electromagnetic Effects
- Conducting polymers and applications
- Cancer Mechanisms and Therapy
Fudan University
2023-2025
Eye & ENT Hospital of Fudan University
2023-2025
Beijing City University
2024-2025
East China Normal University
2018-2024
Soochow University
2024
First Affiliated Hospital of Soochow University
2024
State Key Laboratory of Medical Neurobiology
2023
Shanghai Institute of Technology
2022
Center for Life Sciences
2017
Tsinghua University
2017
Abstract Gene therapy is a promising approach for hereditary deafness. We recently showed that unilateral AAV1-hOTOF gene with dual adeno-associated virus (AAV) serotype 1 carrying human OTOF transgene safe and associated functional improvements in patients autosomal recessive deafness 9 (DFNB9). The protocol was subsequently amended approved to allow bilateral administration. Here we report an interim analysis of the single-arm trial investigating safety efficacy binaural five pediatric...
High-density genetic map provides an essential framework for accurate and efficient genome assembly QTL fine mapping. Construction of high-density maps appears more feasible since the advent next-generation sequencing (NGS), which eases SNP discovery high-throughput genotyping large population. In this research, a high density cucumber (Cucumis sativus) was successfully constructed across F2 population by recently developed specific length amplified fragment (SLAF-seq) method. total, 18.69...
Cucumber is an agriculturally and economically important vegetable crop worldwide. Fruit flesh thickness trait for cucumber also a central determinant of yield, yet little known about the underlying mechanism this trait. In study, bulked segregant analysis (BSA) combined with specific length amplified fragment sequencing (SLAF-seq) was applied to finely map gene that underlies fruit in cucumber. A 0.19-Mb-long quantitative locus on chromosome 2 controlling (QTL fft2.1) identified further...
Abstract Base editors, including dual base are innovative techniques for efficient conversions in genomic DNA. However, the low efficiency of A-to-G conversion at positions proximal to protospacer adjacent motif (PAM) and A/C simultaneous editor hinder their broad applications. In this study, through fusion ABE8e with Rad51 DNA-binding domain, we generate a hyperactive ABE (hyABE) which offers improved editing region (A 10 -A 15 ) PAM, 1.2- 7-fold improvement compared ABE8e. Similarly,...
Abstract Weedy rice ( Oryza spp.) is a semi‐domesticated genetic resource. In high‐latitude paddy ecosystems, weedy tends to head earlier than accompanied japonica rice. However, the heading allele genes underlying this de‐domestication, which crucial for understanding basis and repeatability of heading, remain largely unexplored. Therefore, study aims investigate allelic variation nine using 46 from three areas in northeast China (weedy [WRNC]) 32 their accompanying sativa L.) varieties....
RNA intereferencing (RNAi) pathway regulates antiviral immunity and mediates plant growth development. Despite considerable research efforts, a few components in RNAi have been revealed, including ARGONAUTEs (AGOs), DICER-LIKEs (DCLs), RNA-dependent polymerase 1 6 (RDR1/6), ALTERED MERISTEM PROGRAM (AMP1). In this study, we performed forward genetic screening for enhancers of rdr6 via inoculation CMV2aTΔ2b, 2b-deficient Cucumber Mosaic Virus that is unable to suppress RNAi-mediated immunity....
To diagnose pneumoconiosis using a computer-aided diagnosis system based on digital chest radiographs.Lung fields were first extracted by combining the traditional Otsu-threshold method with morphological reconstruction radiographs (DRs), and then subdivided into six non-overlapping regions (region (a-f)). Twenty-two wavelet-based energy texture features calculated exclusively from each region selected decision tree algorithm. A support vector machine (SVM) linear kernel was trained samples...
Background Artificial neural network (ANN) has demonstrated the ability to assimilate information from multiple sources enable detection of subtle and complex patterns. In this research, we evaluated an ANN model in diagnosis pancreatic cancer using serum markers. Methods retrospective analysis, 913 specimens collected at Department General Surgery Beijing Friendship Hospital were analyzed for carbohydrate antigen 19-9 (CA19-9), 125 (CA125), carcinoembryonic (CEA). The three tumor marker...
Flexible functional electrode materials composed of chemically linked conducting polymer–biopolymer composites for smart device applications are presented.
Abstract A high seeds‐per‐pod value is a crucial component of soybean seed yield, but reliable information lacking on the inheritance and early selection for four‐seeded pod (4SP) trait. The 4SP was followed in crosses originating from an EMS‐derived mutant line (E182, 15% 4SP) with its parental cultivar, ‘Ludou No. 4’ (no 4SP), efficiencies both molecular morphological marker were determined. plants two F 2 populations (with E182 as one parents) showed segregation ratio 3 : 1 (low to SP...