Abstract Jasmonates (JAs) mediate plant responses to insect attack, wounding, pathogen infection, stress, and UV damage regulate fertility, anthocyanin accumulation, trichome formation, many other developmental processes. Arabidopsis thaliana Jasmonate ZIM-domain (JAZ) proteins, substrates of the CORONATINE INSENSITIVE1 (COI1)–based SCFCOI1 complex, negatively these responses. Little is known about molecular mechanism for JA regulation accumulation initiation. In this study, we revealed that...

Abstract The Arabidopsis thaliana F-box protein CORONATINE INSENSITIVE1 (COI1) perceives jasmonate (JA) signals and subsequently targets the Jasmonate-ZIM domain proteins (JAZs) for degradation by SCFCOI1-26S proteasome pathway to mediate various jasmonate-regulated processes, including fertility, root growth, anthocyanin accumulation, senescence, defense. In this study, we screened JAZ-interacting from an cDNA library in yeast two-hybrid system. MYB21 MYB24, two R2R3-MYB transcription...

Precise coordination between stamen and pistil development is essential to make a fertile flower. Mutations impairing filament elongation, pollen maturation, or anther dehiscence will cause male sterility. Deficiency in plant hormone gibberellin (GA) causes sterility due accumulation of DELLA proteins, GA triggers degradation promote development. jasmonate (JA) also However, little known about the relationship JA controlling Here, we show that MYB21, MYB24, MYB57 are GA-dependent...

Abstract Plants have evolved sophisticated mechanisms for integration of endogenous and exogenous signals to adapt the changing environment. Both phytohormones jasmonate (JA) ethylene (ET) regulate plant growth, development, defense. In addition synergistic regulation root hair development resistance necrotrophic fungi, JA ET act antagonistically gene expression, apical hook curvature, defense against insect attack. However, molecular mechanism such antagonism between signaling remains...

Abstract Stamens are the plant male reproductive organs essential for fertility. Proper development of stamens is modulated by environmental cues and endogenous hormone signals. Deficiencies in biosynthesis or perception phytohormone jasmonate (JA) attenuate stamen development, disrupt fertility, abolish seed production Arabidopsis thaliana. This study revealed that JA-mediated regulated a bHLH-MYB complex. The IIIe basic helix-loop-helix (bHLH) transcription factor MYC5 acts as target JAZ...

Plants have evolved sophisticated systems for adaptation to their natural habitat. In response developmental and environmental cues, plants produce perceive jasmonate (JA) signals, which induce degradation of JASMONATE-ZIM-Domain (JAZ) proteins derepress the JAZ-repressed transcription factors regulate diverse aspects defense responses processes. Here, we identified bHLH subgroup IIId (bHLH3, bHLH13, bHLH14 bHLH17) as novel targets JAZs. These act repressors function redundantly negatively...

Plants initiate leaf senescence to relocate nutrients and energy from aging leaves developing tissues or storage organs for growth, reproduction, defense. Leaf senescence, the final stage of development, is regulated by various environmental stresses, developmental cues, endogenous hormone signals. Jasmonate (JA), a lipid-derived phytohormone essential plant defense serves as an important signal activate senescence-associated gene expression induce senescence. This study revealed one...

Abstract Integration of diverse environmental and endogenous signals to coordinately regulate growth, development, defense is essential for plants survive in their natural habitat. The hormonal gibberellin (GA) jasmonate (JA) antagonistically synergistically aspects plant defense. GA JA induce initiation trichomes, which assist seed dispersal act as barriers protect against insect attack, pathogen infection, excessive water loss, UV irradiation. However, the molecular mechanism underlying...

Summary In response to jasmonates (JAs), the JA receptor Coronatine Insensitive 1 (COI1) recruits JA‐zinc‐finger inflorescence meristem (ZIM)‐domain (JAZ) family repressors for destruction regulate plant growth, development, and defense. As Arabidopsis encodes 13 JAZ repressors, their functional specificity, diversity, redundancy in JA/COI1‐mediated responses remain unclear. We generated a broad range of jaz mutants based on phylogenetic relationship investigate roles responses. The group I...

Salt stress is a major abiotic which severely hinders crop production. However, the regulatory network controlling tomato resistance to salt remains unclear. Here, we found that WRKY transcription factor WRKY57 acted as negative regulator in response by directly attenuating of salt-responsive genes (SlRD29B and SlDREB2) an ion homeostasis gene (SlSOS1). We further identified two VQ-motif containing proteins SlVQ16 SlVQ21 SlWRKY57-interacting proteins. positively, while negatively modulated...

The phytohormone jasmonates (JAs) regulate various defense responses and diverse developmental processes including stamen development fertility. Previous studies showed that JA induces CORONATINE INSENSITIVE 1-mediated degradation of ZIM-domain (JAZ) proteins, activates the MYB transcription factors (such as MYB21 MYB24) to development. In this study, we further uncover mechanism underlying how MYB24 interacts with JAZs control JA-regulated We show N-terminus MYB21/24 10 out 12 JAZ proteins...

Jasmonates (JAs), lipid-derived phytohormones, regulate plant growth, development and defenses against biotic stresses. CORONATINE INSENSITIVE1 perceives bioactive JA recruits JASMONATE ZIM-DOMAIN (JAZ) proteins for ubiquitination subsequent degradation via the 26S proteasome, which de-represses JAZ-targeted transcription factors that diverse responses. Recent studies showed Arabidopsis basic helix-loop-helix factor MYC5 interacts with JAZs regulates stamen development. However, whether...

Parasitic root-knot nematodes (RKNs) cause a severe reduction in crop yield and seriously threaten agricultural production. The phytohormones jasmonates (JAs) are important signals regulating resistance to multiple biotic abiotic stresses. However, the molecular mechanism for JAs-regulated defense against RKNs tomato remains largely unclear. In this study, we found that transcription factor SlWRKY45 interacted with most JA-ZIM domain family proteins (JAZs), key repressors of JA signaling....

The phytohormones ethylene (ET) and jasmonate (JA) regulate plant development, growth, defense responses; however, the molecular basis for their signaling crosstalk is unclear. Here, we show that JA-ZIM-domain (JAZ) proteins, which repress JA signaling, trichome initiation/branching anthocyanin accumulation, inhibit transcriptional activity of basic helix-loop-helix (bHLH)-MYB members (GLABRA3 (GL3)-GL1 TRANSPARENT TESTA 8 (TT8)-MYB75) WD-repeat/bHLH/MYB (WBM) complexes. ET-stabilized...

Abstract Botrytis cinerea is one of the most widely distributed and harmful pathogens worldwide. Both phytohormone jasmonate (JA) VQ motif-containing proteins play crucial roles in plant resistance to B. cinerea. However, their crosstalk unclear, especially tomato (Solanum lycopersicum). In this study, we found that VQ15 was highly induced upon infection localized nucleus. Silencing SlVQ15 using virus-induced gene silencing reduced Overexpression enhanced cinerea, while disruption clustered...

Abstract In the presence of pathogenic bacteria, plants close their stomata to prevent pathogen entry. Intracellular nucleotide-binding leucine-rich repeat (NLR) immune receptors recognize effectors and activate effector-triggered responses. However, regulatory molecular mechanisms stomatal immunity involving NLR are unknown. Here, we show that Nicotiana benthamiana RPW8-NLR central receptor ACTIVATED DISEASE RESISTANCE 1 (NbADR1), together with key proteins ENHANCED SUSCEPTIBILITY (NbEDS1)...

Photoperiod and temperature are two important environmental factors that influence the heading-date of rice. Although photoperiod on heading has been extensively reported in rice, molecular mechanism for control remains unknown. This study reports an early mutant derived from tissue culture lines rice investigates wild type different treatments. The linkage analysis showed phenotype cosegregated with Hd1 locus. Sequencing found contained insertions several single-base substitutions caused a...

Abstract Background Gibberellin (GA) and jasmonate (JA) are two essential phytohormones for filament elongation in Arabidopsis . GA JA trigger degradation of DELLAs JASMONATE ZIM-domain (JAZ) proteins through SCF SLY1 COI1 separately to activate elongation. In pathway, JAZs interact with MYB21 MYB24 control However, little is known how regulate Results Here we showed that MYB24, R2R3 domains responsible interaction DELLAs. Furthermore, demonstrated DELLA JAZ coordinately repress the...

RNA intereferencing (RNAi) pathway regulates antiviral immunity and mediates plant growth development. Despite considerable research efforts, a few components in RNAi have been revealed, including ARGONAUTEs (AGOs), DICER-LIKEs (DCLs), RNA-dependent polymerase 1 6 (RDR1/6), ALTERED MERISTEM PROGRAM (AMP1). In this study, we performed forward genetic screening for enhancers of rdr6 via inoculation CMV2aTΔ2b, 2b-deficient Cucumber Mosaic Virus that is unable to suppress RNAi-mediated immunity....

The phytohormone jasmonates (JAs) regulate plant development, growth, secondary metabolism, and defense responses. JAs act through CORONATINE INSENSITIVE1 (COI1) to induce the degradation of JA ZIM-domain (JAZ) proteins, activate JAZ-repressed transcription factors response. We previously showed that basic helix-loop-helix (bHLH) MYB members WD-repeat/bHLH/MYB complex interacted with JAZs mediated JA-induced anthocyanin accumulation trichome initiation. In this study, we C-terminal domain...