A5028969814- Plant Virus Research Studies
- Plant Pathogenic Bacteria Studies
- Plant and Fungal Interactions Research
- CRISPR and Genetic Engineering
- Biosensors and Analytical Detection
- Genetically Modified Organisms Research
- Phytoplasmas and Hemiptera pathogens
- Plant-Microbe Interactions and Immunity
- Insect-Plant Interactions and Control
- Insect Resistance and Genetics
- Transgenic Plants and Applications
- Advanced biosensing and bioanalysis techniques
- Plant Disease Resistance and Genetics
- Insect behavior and control techniques
- Genomics and Phylogenetic Studies
- Forest Insect Ecology and Management
- Cocoa and Sweet Potato Agronomy
- Insect and Pesticide Research
- Plant Pathogens and Fungal Diseases
- Identification and Quantification in Food
- Plant tissue culture and regeneration
- Neurobiology and Insect Physiology Research
- Insect and Arachnid Ecology and Behavior
- Insect symbiosis and bacterial influences
- Vector-Borne Animal Diseases
Chinese Academy of Inspection and Quarantine
2015-2024
China Agricultural University
2011-2023
Chinese Academy of Tropical Agricultural Sciences
2022
Jiangnan University
2009-2010
Beijing University of Posts and Telecommunications
2010
Shanghai Entry-Exit Inspection and Quarantine Bureau
2010
Shandong Entry-Exit Inspection and Quarantine Bureau
2010
State Key Laboratory of Food Science and Technology
2009-2010
Fujian Entry - Exit Inspection and Quarantine Bureau
2009
Hunan Entry-Exit Inspection and Quarantine Bureau
2009
Adapter trimming is a prerequisite step for analyzing next-generation sequencing (NGS) data when the reads are longer than target DNA/RNA fragments. Although typically used in small RNA sequencing, adapter also widely other applications, such as genome DNA and transcriptome RNA/cDNA where fragments shorter read sometimes obtained because of limitations NGS protocols. For newly emerged Nextera long mate-pair (LMP) protocol, junction adapters located middle all properly constructed fragments;...
Virus infection of plants may induce a variety disease symptoms. However, little is known about the molecular mechanism systemic symptom development in infected plants. Here we performed first next-generation sequencing study to identify gene expression changes associated with tobacco (Nicotiana tabacum cv. Xanthi nc) induced by M strain Cucumber mosaic virus (M-CMV). Analysis transcriptome RNA-Seq identified 95,916 unigenes, 34,408 which were new transcripts database searches. Deep was...
Chiral properties of nanoscale materials are importance as they dominate interactions with proteins in physiological environments; however, have rarely been investigated. In this study, a systematic investigation is conducted for the adsorption behaviors bovine serum albumin (BSA) onto chiral surfaces gold nanoparticles (AuNPs), involving multiple techniques and molecular dynamic (MD) simulation. The BSA both L- D-chiral AuNPs shows discernible differences thermodynamics, orientation,...
Abstract Paris is famous in China for its medicinal value and has been included the Chinese Pharmacopoeia. Inaccurate identification of these species could confound their effective exploration, conservation, domestication. Due to plasticity morphological characteristics, correct among remains problematic. In this regard, we report complete chloroplast genome P . thibetica rugosa develop highly variable molecular markers. Comparing three genomes, sought out most regions best cpDNA barcodes...
Rice is the most important crop in world as staple food for over half of population. The wild species Oryza represent an enormous gene pool genetic improvement rice cultivars. Accurate and rapid identification these critical effective utilization germplasm. In this study, we developed valuable chloroplast molecular markers by comparing genomes identification. Four were newly sequenced on Illumina HiSeq platform other 14 from Genbank simultaneously taken into consideration comparative...
Colorado Potato Beetle (CPB) is a devastating invasive pest of potato both in its native North America and now across Eurasia. It also damages eggplant, tomato feeds on several wild species the Solanaceae, such as S. eleagnifolium rostratum Dunal (SR). Since first categorized 1864, CPB has spread rapidly America, Europe Asia. In light invasiveness economic importance, it necessary to study how climate change host availability may alter distribution CPB. Maximum Entropy (MaxEnt) models were...
Abstract Bactrocera dorsalis is an invasive polyphagous pest causing considerable ecological and economic damage worldwide. We report a high-quality chromosome-level genome assembly combine various transcriptome data to explore the molecular mechanisms of its rapid adaptation new environments. The expansions DDE transposase superfamily key gene families related environmental enrichment expanded unique in metabolism defence response pathways explain adaptability. relatively high but not...
Phytoplasmas are plant pathogenic bacteria that have no cell wall and responsible for major crop losses throughout the world. Phytoplasma-infected plants show a variety of symptoms mechanisms they use to physiologically alter host considerable interest, but poorly understood. In this study we undertook detailed analysis Paulownia infected by witches'-broom (PaWB) Phytoplasma using high-throughput mRNA sequencing (RNA-Seq) digital gene expression (DGE). RNA-Seq identified 74,831 unigenes,...
Abstract Cucumber mosaic virus (CMV) infection could induce symptoms on a wide-range of host plants. However, there is still limited information regarding the molecular mechanism underlying development symptoms. In this study, coat protein (CP) was confirmed as symptom determinant by exchanging CP between chlorosis inducing CMV-M strain and green-mosaic CMV-Q strain. A yeast two-hybrid analysis bimolecular fluorescence complementation revealed that chloroplast ferredoxin I (Fd I) interacted...
Abstract Digital PCR has developed rapidly since it was first reported in the 1990s. It recently that an improved method facilitated detection of genetically modified organisms (GMOs). However, to use this method, samples must be pretreated, which could introduce inaccuracy into results. In our study, we explored a pretreatment-free digital for screening GMOs. We chose CaMV35s promoter and NOS terminator as templates assay. To determine specificity 9 events GMOs were collected, including...
The pinewood nematode, Bursaphelenchus xylophilus, has caused significant damage to pine plantations both in East Asia and North America is an important quarantine organism. A real-time polymerase chain reaction (PCR) assay was developed detect B. xylophilus. set of primers probe specific for xylophilus designed target the ribosomal DNA internal transcribed spacer region. Optimal primer concentration, Mg 2+ extension temperature were 400 nM, 3.0 mM, 60°C, respectively. highly sensitive,...
With the increasing development of genetically modified organism (GMO) detection techniques, polymerase chain reaction (PCR) technique has been mainstay for GMO detection. An oligonucleotide microarray is a glass chip to surface which an array oligonucleotides was fixed as spots, each containing numerous copies sequence-specific probe that complementary gene interest. So it used detect ten or more targets synchronously. In this research, event-specific strategy based on unique and specific...
Plants have evolved multiple mechanisms to respond viral infection. These responses been studied in detail at the level of host immune response and antiviral RNA silencing (RNAi). However, possibility epigenetic reprogramming has not thoroughly investigated. Here, we identified role DNA methylation during infection performed reduced representation bisulfite sequencing (RRBS) on tissues Cucumber mosaic virus (CMV)-infected Nicotiana tabacum various developmental stages. Differential...
Sorghum comprises 31 species that exhibit considerable morphological and ecological diversity. The phylogenetic relationships among still remain unresolved due to lower information on the traditional DNA markers, which provides a limited resolution for identifying species. In this study, we sequenced complete chloroplast genomes of sudanense S. propinquum analyzed published bicolor timorense retrieve valuable molecular resources Sorghum. ranged in length from 140,629 140,755 bp, their gene...