A5042239117- CRISPR and Genetic Engineering
- Genetically Modified Organisms Research
- Insect Resistance and Genetics
- Animal Genetics and Reproduction
- Plant Gene Expression Analysis
- Molecular Biology Techniques and Applications
- Plant Virus Research Studies
- Plant biochemistry and biosynthesis
- RNA and protein synthesis mechanisms
- Identification and Quantification in Food
- Plant Molecular Biology Research
- Insect Pest Control Strategies
- Glutathione Transferases and Polymorphisms
- Biosensors and Analytical Detection
- Genetic diversity and population structure
- Viral Infectious Diseases and Gene Expression in Insects
- Advanced biosensing and bioanalysis techniques
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Epigenetics and DNA Methylation
- Plant Reproductive Biology
- Insect behavior and control techniques
- Geochemistry and Elemental Analysis
- Genetic and phenotypic traits in livestock
- Infant Nutrition and Health
Southwest Minzu University
2021-2025
State Ethnic Affairs Commission
2021-2025
Chinese Academy of Inspection and Quarantine
2013-2024
Shenyang Agricultural University
2017-2024
China Agricultural University
2020-2024
Sichuan Agricultural University
2024
Research Center for Eco-Environmental Sciences
2024
Chinese Academy of Sciences
2024
University of Chinese Academy of Sciences
2024
Hebei Agricultural University
2024
Quantitative real-time PCR (qRT-PCR), a primary tool in gene expression analysis, requires an appropriate normalization strategy to control for variation among samples.The best option is compare the mRNA level of target with that reference gene(s) whose stable across various experimental conditions.In this study, profiles eight candidate genes from diamondback moth, Plutella xylostella, were evaluated under diverse conditions.RefFinder, web-based analysis tool, integrates four major...
Abstract The transition of embryonic stem cell (ESC) pluripotency to differentiation is accompanied by an expansion mRNA and proteomic diversity. Post-transcriptional regulation ESCs critically governed type-specific splicing. However, little known about the splicing factors molecular mechanisms directing ESC early lineage differentiation. Our study identifies RNA binding motif protein 24 (Rbm24) as a key regulator that plays essential role in controlling post-transcriptional networks during...
The larval midgut of diamondback moth, Plutella xylostella, is a dynamic tissue that interfaces with diverse array physiological and toxicological processes, including nutrient digestion allocation, xenobiotic detoxification, innate adaptive immune response, pathogen defense.Despite its enormous agricultural importance, the genomic resources for P. xylostella are surprisingly scarce.In this study, Bt resistant strain was subjected to in-depth transcriptome analysis identify genes gene...
Abstract Digital PCR has developed rapidly since it was first reported in the 1990s. It recently that an improved method facilitated detection of genetically modified organisms (GMOs). However, to use this method, samples must be pretreated, which could introduce inaccuracy into results. In our study, we explored a pretreatment-free digital for screening GMOs. We chose CaMV35s promoter and NOS terminator as templates assay. To determine specificity 9 events GMOs were collected, including...
Societal Impact Statement Advancements in science and technology enable us to address the challenges of times: hunger, malnutrition, climate change, dwindling natural resources. One most significant innovations food agriculture is advent genetic engineering, which may help aforementioned challenges. Millions people could benefit from application genetically engineered crops food. However, it vital that biosafety assessments unintended effects are carried out identify any potential risks...
There are substantial carbon exchange fluxes among soil, vegetation and atmosphere in the terrestrial ecosystems, which highly relevant to global climate changes.Mycorrhizal fungi can form symbiotic associations with most plants, linking above-and below-ground ecosystems through mineral nutrient-carbon exchange; thus, mycorrhizal play crucial roles cycling.This review summarized involvements of cycling processes, including input, formation, stabilization, decomposition soil organic...
Abstract BACKGROUND The cotton bollworm, Helicoverpa armigera (Hübner), and the beet armyworm, Spodoptera exigua are two major polyphagous lepidopteran pests of cultivated crops. They develop various levels resistance to many frequently applied broad‐spectrum insecticides. Here, larval susceptibilities a laboratory population six field‐collected populations per pest from Hunan Province, China three insecticides were determined using standard leaf‐dipping method in laboratory. Field‐plot...
Resistance to insecticidal Bacillus thuringiensis (Bt) toxins has arisen in multiple populations of the worldwide Brassica pest Plutella xylostella (L.). To help elucidate mechanism resistance Bt Cry1Ac toxin a population from Florida, two pairs near-isogenic lines (NILs) were developed.NILs generated using either backcross or recombinant inbred line methodologies and evaluated for near-isogenicity with inter-simple-sequence-repeat (ISSR) markers. Backcross BC6F4 maintained similar level...
Abstract Red clay widely occupies the seafloor of pelagic environments in middle latitudes, and potentially preserves long paleoceanographic records. We conducted a rock-magnetic study Pacific Ocean red to elucidate paleoenvironmental changes. Three piston cores from western North IODP Hole U1365A South were studied here. Principal component analyses applied first-order reversal curve diagrams (FORC-PCA) reveals three magnetic components (endmembers EM1 through EM3) core Pacific. EM1, which...
Abstract The presence of a leafy head is vital agronomic trait that facilitates the evaluation yield and quality Chinese cabbage. A non-heading mutant ( nhm1 ) was identified in an ethyl methanesulfonate mutagenesis population heading cabbage double haploid line FT. Segregation analysis revealed single recessive gene, Brnhm1 , controlled phenotype. Using MutMap, Kompetitive allele-specific PCR, cloning analyses, we demonstrated BraA07g042410.3C which encodes ent -kaurene synthase involved...
Genetically modified (GM) organisms have been developed for decades. However, unintended effects are the main concerns of safety assessment that needs to be carefully investigated. Here, eight varieties GM rice were in China selected assess through transcriptome and metabolism. There 2892-8758 differentially expressed genes (DEGs) 7-50 metabolites at significant level between their isogenic counterparts, which far fewer than traditional varieties. The function enrichment analysis showed...
Invasive species can be detrimental to a nation's ecology, economy and human health. Rapid accurate diagnostics are critical limit the establishment spread of exotic organisms. The increasing rate biological invasions relative taxonomic expertise available generates demand for high-throughput, DNA-based methods identification. We designed species-specific qPCR primer probe combinations 27 economically important tephritidae in six genera (Anastrepha, Bactrocera, Carpomya, Ceratitis, Dacus...
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) system has been widely applied in functional genomics research and plant breeding. In contrast to the off-target studies of mammalian cells, there is little evidence for common occurrence sites plants a great need exists accurate detection editing sites. Here, we summarized precision CRISPR/Cas9-mediated mutations 281 targets found that preference single nucleotide deletions/insertions...
Abstract The red inner leaf phenotype of ornamental kale is due to anthocyanin accumulation and an important agronomic trait. This controlled by a single dominant nuclear gene, Re . An F 2 mapping population from cross between white lines was developed, found be located BYI n3 C09‐In51 on chromosome nine (C09) at distance 0.003 1.8 cM, respectively. Twenty‐six genes were identified in this region, but none related the biosynthesis pathway. On basis information Ensembl Plants, transcriptome...