This work details the preparation of graphene quantum dots and investigates their membrane permeabilities transport properties across MDCK cell monolayer.

Abstract Cucumber mosaic virus (CMV) infection could induce symptoms on a wide-range of host plants. However, there is still limited information regarding the molecular mechanism underlying development symptoms. In this study, coat protein (CP) was confirmed as symptom determinant by exchanging CP between chlorosis inducing CMV-M strain and green-mosaic CMV-Q strain. A yeast two-hybrid analysis bimolecular fluorescence complementation revealed that chloroplast ferredoxin I (Fd I) interacted...

Contrast induced-acute kidney injury (CI-AKI) is one of the most common causes acute (AKI) in hospitalized patients. Mitophagy, selective elimination mitochondria via autophagy, an important mechanism mitochondrial quality control physiological and pathological conditions. In this study, we aimed to determine effects iohexol iodixanol on reactive oxygen species (ROS), mitophagy potential role CI-AKI cell models.Cell viability was measured by counting kit-8. Cell apoptosis, ROS membrane were...

Abstract Equine piroplasmosis (EP) is a severe disease of horses caused by the tick-borne protozoa Theileria equi ( T. ) and Babesia caballi B. ). Infectious carriers are not always symptomatic, meaning there risk to non-enzootic areas. Regulatory tests for EP include sero-epidemiological methods equine babesiosis, but these lack specificity due cross-reactivity with other species. In this study, we present real-time quantitative recombinase polymerase amplification (qRPA) method fast...

Several tobamoviruses cause substantial economic losses to tomato and pepper crops globally, especially the mild mosaic virus (PMMoV), brown rugose fruit (ToBRFV), (ToMV), mottle (ToMMV). A fast accurate detection method is essential for identification. An all-in-one reaction combining a one-step reverse-transcription recombinase-aided amplification (RT-RAA) CRISPR/Cas12a-based lateral flow assay in one mixture was developed rapidly screen accurately differentiate among these four field...

Abstract The production of reactive oxygen species (ROS) forms part the defense reaction plants against invading pathogens. ROS have multifaceted signaling functions in mediating establishment multiple responses. To verify whether hydrogen peroxide (H 2 O ) contributes to plant virus infection and development induced symptoms, we used fluorescence monitor generation H confocal laser scanning microscopy (CLSM) investigate subcellular distribution leaves. In this study, M strain Cucumber...

Contrast media (CM)-induced nephropathy (CIN) is a serious complication of intravascularly applied radiocontrast media. At present, no drugs have been approved for the prevention CIN. The present study aimed to explore effects and potential mechanisms atorvastatin on iodinated CM-induced cytotoxicity in human proximal renal tubular epithelial cells. cytotoxic effect iohexol (50, 100 200 mg I/ml) protective pretreatment (1, 20 40 µM) were assessed. was evaluated via MTT cell viability lactate...

Iodiated contrast-induced nephropathy (CIN) is a serious complication of contrast-enhanced imaging. The aim this study was to evaluate the diagnostic sensitivities and specificities serum cystatin C (sCys C) creatinine (sCr) for CIN further investigate difference incidence, risk factors, in-hospital 3-month prognosis according sCys criteria sCr criteria.We prospectively evaluated 213 patients who underwent angiography. concentrations were detected before at 48 hours, 72 hours after...

Globodera rostochiensis is an important quarantine pest, it causes serious potato yield losses annually. Reliable and rapid molecular detection of G. pivotal to effective early disease diagnosis managements. Herein, recombinase polymerase amplification integrated with lateral flow assays method (RPA-LFA) was developed target the internal transcribed spacer nuclear ribosomal DNA (ITS rDNA) golden cyst nematode (G. rostochiensis), which allowed for this from crude extracts cysts juveniles...

An easy-to-implement strategy to identify<italic>Solanum elaeagnifolium</italic>by utilizing recombinase polymerase amplification (RPA) technology was developed.

Abstract Virus infection in plants involves necrosis, chlorosis, and mosaic. The M strain of cucumber mosaic virus (M‐CMV) has six distinct symptoms: vein clearing, mosaic, partial green recovery, complete secondary Chlorosis indicates the loss chlorophyll which is highly abundant plant leaves plays essential roles photosynthesis. Blue native/SDS‐PAGE combined with mass spectrum was performed to detect location virus, proteomic analysis chloroplast isolated from virus‐infected quantify...

Graphyne, a newly discovered carbon nanomaterial, has received increasing attention as potential biomedical material. Although β-rich peptide scaffolds have been implicated in range of neurodegenerative diseases, the mechanisms by which toxic peptides assemble and mediate neuropathic effects remain poorly understood. Modeling interaction between graphyne is crucial for understanding nanobiological or nanotoxicity safe design nanomedicine Herein, we investigate on assembly Aβ33–42 molecular...

Zika virus (ZIKV) infection can result in a birth defect of the brain called microcephaly and other severe fetal defects. ZIKV enters susceptible host cells by endocytosis, which is mediated interaction envelope (E) glycoprotein with cellular surface receptor molecules. However, factors that used to gain access remains elusive. Here, we report extracellular domain integrin beta 4 (ITGB4) an entry factor ZIKV. ITGB4 mediates directly interacting E ZIKV, knockout hampers binding replication...

To control plant viruses, viral RNA silencing suppressors are important drug targets due to their key roles in interfering antiviral silencing. Here we have presented a strategy, combining virtual and experimental screening, discover the inhibitors of suppressor. By docking 157 026 compounds from natural product library into P19 model, eighteen candidate were selected. Candidates VS2, VS12, VS14 VS15 displayed strong binding ability surface plasmon resonance imaging assay with KD values...

As a traditional Chinese medicine external treatment method, acupuncture is characterized by simple operation, significant effect and few side effects. Tong-Xie-Yao-Fang (TXYF), patent medicine, combined with has been widely used on treating Diarrhea Predominant Irritable Bowel Syndrome (IBS-D). However, the efficacy safety of TXYF for IBS-D are unclear. This study aims to investigate verify IBS-D.

To investigate the incidence, risk factors and prognosis for contrast-induced acute kidney injury (CI-AKI) according to ESUR KDIGO criteria in patients undergoing angiography. We evaluated 260 angiography and/or intervention therapy from April 2011 January 2012 Second Xiangya Hospital of Central South University. All received low-osmolality contrast agent (ioversol). Serum creatinine was measured before or at 48 72 h after procedure. The multivariate logistic regression used analyze CI-AKI....

<title>Abstract</title> Background Trypanosomiasis is a highly lethal infectious disease caused by trypanosome, leading to severe social and economic burden worldwide. Due the lack of mechanism research, application promising nanomaterials nanotechnologies in treatment trypanosomiasis limited. Results Herein, toxicological effects induced graphene quantum dots (GQDs) on<italic>T. brucei</italic>and underlying are investigated. First, biological/cytotoxic evaluated, including endotytosis,...

Graphyne, a newly discovered carbon nanomaterial, has received increasing attention as potential biomedical material. Although β-rich peptide scaffolds have been implicated in range of neurodegenerative diseases, the mechanisms by which toxic peptides assemble and mediate neuropathic effects remain poorly understood. Modeling behavior graphyne interacting with is crucial to understand predict efficacy drug carrier, therapeutic agent, biological effect or toxicity. Herein, we investigate on...