A5038848934- Plant Reproductive Biology
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Chromosomal and Genetic Variations
- Aquaculture Nutrition and Growth
- Genetic Mapping and Diversity in Plants and Animals
- Reproductive biology and impacts on aquatic species
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Peanut Plant Research Studies
- Legume Nitrogen Fixing Symbiosis
- Aquaculture disease management and microbiota
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Plant Stress Responses and Tolerance
- Plant responses to water stress
- Glutathione Transferases and Polymorphisms
- Agronomic Practices and Intercropping Systems
- Biofuel production and bioconversion
- Plant-Microbe Interactions and Immunity
- Animal Genetics and Reproduction
- Plant biochemistry and biosynthesis
- Rice Cultivation and Yield Improvement
- Plant Surface Properties and Treatments
- Nitrogen and Sulfur Effects on Brassica
- Coconut Research and Applications
South China Agricultural University
2011-2024
Ministry of Agriculture and Rural Affairs
2023-2024
Key Laboratory of Guangdong Province
2016-2023
Hainan University
2023
Zhejiang Institute of Freshwater Fisheries
2017-2021
Huazhong Agricultural University
2017-2021
University of Florida
2017-2020
State Key Laboratory For Conservation and Utilization of Subtropical Agro-Bioresources
2012-2020
Center for Excellence in Molecular Cell Science
2014
Chinese Academy of Sciences
2014
Abstract Hybrid rice breeding offers an important strategy to improve production, in which the cultivation of a male sterile line is key success cross-breeding. CRISPR/Cas9 systems have been widely used target-site genome editing, whereas their application for crop genetic improvement has rarely reported. Here, using system, we induced specific mutations TMS5 , most applied thermo-sensitive genic sterility (TGMS) gene China, and developed new “transgene clean” TGMS lines. We designed 10...
Proteins of the ARGONAUTE (AGO) family function in epigenetic regulation gene expression. Although rice (Oryza sativa) genome encodes 19 predicted AGO proteins, few their functions have thus far been characterized. Here, we show that protein OsAGO2 regulates anther development rice. was highly expressed anthers. Knockdown led to overaccumulation reactive oxygen species (ROS) and abnormal development, causing premature initiation tapetal programmed cell death (PCD) pollen abortion. The...
Cuticular wax is a class of organic compounds that comprises the outermost layer plant surfaces. Plant cuticular wax, last barrier self-defense, plays an important role in growth and development. The OsGL1-6 gene, member fatty aldehyde decarbonylase gene family, highly homologous to Arabidopsis CER1, which involved biosynthesis. However, whether participates biosynthesis remains unknown. In this study, antisense-RNA vector driven by its own promoter was constructed introduced into rice...
Abstract Uridine diphosphate (UDP)-sugars are important metabolites involved in the biosynthesis of polysaccharides and may be signaling molecules. UDP-glucose 4-epimerase (UGE) catalyzes interconversion between UDP-Glc UDP-Gal, whose biological function rice (Oryza sativa) fertility is poorly understood. Here, we identify characterize botryoid pollen 1 (bp1) mutant show that BP1 encodes a UGE regulates UDP-sugar homeostasis, thereby controlling development anthers. The loss led to massive...
Summary Thermo‐sensitive genic male sterile (TGMS) lines are the core of two‐line hybrid rice ( Oryza sativa ). However, elevated or unstable critical sterility‐inducing temperatures (CSITs) TGMS bottlenecks that restrict development rice. genes and molecular mechanisms controlling CSIT remain unknown. Here, we report CRITICAL STERILITY‐INDUCING TEMPERATURE 2 CSIT2 ) encodes a really interesting new gene (RING) type E3 ligase, thermo‐sensitive sterility 5 tms5 )‐based through...
Summary Inter‐subspecific indica – japonica hybrid rice ( Oryza sativa ) has the potential for increased yields over traditional intra‐subspecies rice, but limited yield of F 1 seed production (FHSP) hinders development breeding. Diurnal flower‐opening time (DFOT) divergence between and been a major contributing factor to this issue, few DFOT genes have cloned. Here, we found that manipulating expression jasmonate (JA) pathway can effectively modulate improve FHSP in rice. Treating cultivar...
Diurnal flower-opening time (DFOT), the of spikelet opening during day, is an important trait for hybrid rice (Oryza sativa L.) seed production. Hybrids between indica and japonica varieties have strong heterosis, but parental lines usually different, nonoverlapping DFOTs. This reduces success production in crosses subspecies, thus hindering utilization inter-subspecies heterosis. However, little known about molecular mechanisms regulating DFOT rice. Here, we obtained with a 1.5 h earlier...
Plant architecture is a major target of rice (Oryza sativa) breeding and selection, but the underlying regulatory networks remain unclear. Here, we overexpressed an OsNAC2 mutant (OErN) that cannot be cleaved by miRNA miR164b. OErN plants had better plant longer panicles, produced more grains. The parental line averaged 12.2 primary 31.5 secondary branches in main panicles; two lines 15.0 15.2 primary, 41.5 44.3 branches. In large-scale field trials, at least 58.62% total grain (by weight)...
Summary Most plant pentatricopeptide repeat (PPR) proteins localize to and function inside plastids mitochondria. However, the of PPRs that only cytoplasm remains unknown. Here, we demonstrated rice ( Oryza sativa ) PPR protein CYTOPLASM‐LOCALIZED PPR1 (OsCPPR1) contributes pollen development localizes cytoplasm. Knocking down OsCPPR1 led abnormal plastid in tapetal cells, prolonged programmed cell death (PCD) tapetum degradation, significantly reduced fertility. Transcriptome analysis...
Cuticle waxes, which are primarily comprised of very-long-chain (VLC) alkanes, play an important role in plant reproductive development. ECERIFERUM1 (CER1) is recognized as the core element for VLC alkane biosynthesis Arabidopsis (Arabidopsis thaliana). However, genes involved rice remain unclear, and alkane-form pathway has still to be further explored. Here, we show that OsCER1, a homology CER1, functions alkanes biosynthesis, also could regulate anther development plastids differentiation...
Summary Environment‐sensitive genic male sterility (EGMS) lines are used widely in two‐line hybrid breeding rice ( Oryza sativa ). At present, photoperiod‐sensitive (PGMS) and thermo‐sensitive (TGMS) predominantly rice, with humidity‐sensitive (HGMS) rarely being reported. Here, it is shown that HUMIDITY‐SENSITIVE GENIC MALE STERILITY 1 HMS1 ), encoding a β‐ketoacyl‐CoA synthase, plays key roles the biosynthesis of very‐long‐chain fatty acids (VLCFAs) HGMS rice. The hms1 mutant displayed...
Abstract The molecular mechanisms of symbiosis in cultivated peanut with a ‘crack entry’ infection process are largely understudied. In this study, we investigated the root transcriptional profiles two pairs non-nodulating (nod−) and nodulating (nod+) sister inbred lines, E4/E5 E7/E6, their nod+ parents, F487A PI262090 during rhizobial nodule initiation by using RNA-seq technology. A total 143, 101, 123, 215, 182, 289 differentially expressed genes (DEGs) were identified nod− E4, E7 E5, E6,...
In early mouse embryogenesis, the distinct enrichment of α‐ketoglutarate (αKG) in blastocysts and L‐2‐hydroxyglutarate (L‐2HG) 2‐cell (2C) embryos serves as a key metabolic signature. While elevated L‐2HG levels inhibit resolution totipotency during transition from 2C stage to blastocyst, role αKG remains elusive. Mouse embryonic stem cells (mESCs) cultured vitro naturally harbor subpopulation that transitions dynamically into 2C‐like totipotent state, providing convenient model investigate...
Abstract Chinese sturgeon ( Acipenser sinensis ) is an endangered species, listed as a grade I protected animal in China. The females rarely successfully develop their gonads from stage II to III captivity, which handicaps the propagation of cultured sturgeon. present study aimed understand effects dietary lipid level on ovarian development and related regulation mechanism female A 24-month feeding trial was conducted with 10-year-old sturgeons ovaries at developmental II, three experimental...
Antisense and RNA interference (RNAi)-mediated gene silencing systems are powerful reverse genetic methods for studying function. Most RNAi antisense experiments used constitutive promoters to drive the expression of RNAi/antisense transgenes; however, several reports showed that were not expressed in all cell types cereal plants, suggesting promoter effective certain tissues/organs. To develop an alternative method complements systems, we constructed and/or transgenes four rice genes using...
C-kit positive (c-kit+) cells are usual tissue-specific stem cells. However, in postnatal testis, undifferentiated spermatogonial (SSCs) c-kit negative (c-kit−) and activation of represents the start SSC differentiation, leaving an intriguing question whether other c-kit+ exist participate development testis. To this end, a feasible system for testicular reconstitution, which specific type can be manipulated, is needed. Here, we first establish de novo morphogenesis testis by subcutaneous...