A5056352263- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- SARS-CoV-2 and COVID-19 Research
- Reproductive Biology and Fertility
- Mitochondrial Function and Pathology
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Metabolism and Genetic Disorders
- Birth, Development, and Health
- Renal and related cancers
- Epigenetics and DNA Methylation
- Animal Genetics and Reproduction
- Trace Elements in Health
- Ovarian function and disorders
- RNA Interference and Gene Delivery
- Endometriosis Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Genetics, Aging, and Longevity in Model Organisms
- Sperm and Testicular Function
- Pregnancy and Medication Impact
- Heavy Metal Exposure and Toxicity
- Virus-based gene therapy research
- Reproductive Health and Technologies
- Prenatal Screening and Diagnostics
- Pregnancy and preeclampsia studies
Anhui Medical University
2021-2025
Ministry of Education of the People's Republic of China
2022-2025
First Affiliated Hospital of Anhui Medical University
2021-2025
Jinan University
2022-2025
Tianjin Academy of Agricultural Sciences
2025
Institute of Crop Sciences
2025
Guangdong Provincial Center for Disease Control and Prevention
2021-2024
Oregon Health & Science University
2018-2024
Anhui Provincial Hospital
2022-2024
Guiyang Medical University
2020-2024
Highlights•A dominant cataract-causing mutation in the Crygc gene is corrected using CRISPR-Cas9•Genetic correction via HDR uses information from endogenous WT allele•Genetic can also occur an exogenous oligo•The rescued mice transmit allele to their progenySummaryThe CRISPR-Cas9 system has been employed generate mutant alleles a range of different organisms. However, so far there have not reports use this for efficient genetic disease. Here we show that with causes cataracts could be by...
Epithelial–mesenchymal transitions (EMTs) are an essential manifestation of epithelial cell plasticity during morphogenesis, wound healing, and tumor progression. Transforming growth factor-β (TGF-β) modulates in these physiological contexts by inducing EMT. Here we report a transcriptome screen genetic programs TGF-β-induced EMT human keratinocytes propose functional roles for extracellular response kinase (ERK) mitogen-activated protein signaling motility disruption adherens junctions. We...
The CRISPR/Cas9 system is an efficient gene-editing method, but the majority of gene-edited animals showed mosaicism, with editing occurring only in a portion cells. Here we show that single gene or multiple genes can be completely knocked out mouse and monkey embryos by zygotic injection Cas9 mRNA adjacent single-guide RNAs (spaced 10-200 bp apart) target key exon each gene. Phenotypic analysis F0 mice following targeted deletion eight on Y chromosome individually demonstrated robustness...
The plant-specific GRAS/SCL transcription factors play diverse roles in plant development and stress responses. In this study, a poplar SCL gene, PeSCL7, was functionally characterized Arabidopsis thaliana, especially with regard to its role abiotic resistance. Expression analysis revealed that PeSCL7 induced by drought high salt stresses, but repressed gibberellic acid (GA) treatment leaves. Transient expression of GFP-PeSCL7 onion epidermal cells the protein localized nucleus. Transgenic...
Emerging evidence has demonstrated that melatonin (MT) plays a crucial role in regulating mammalian reproductive functions. It been reported MT protective effect on polycystic ovary syndrome (PCOS). However, the mechanisms of remain poorly understood. This study aims to explore ovarian function PCOS and elucidate relevant molecular vivo vitro. Here, we first analysed expression levels follicular fluid patients. A significant reduction was noted Intriguingly, reduced correlated with serum...
To describe gene expression changes that characterize the development of diabetic nephropathy, we performed microarray and phenotype analysis on kidneys from db/db mice (a model type 2 diabetes), streptozotocin-induced C57BL/6J 1 nondiabetic controls. Statistical comparisons were implemented based phenotypic outcome characteristics animals. We used weighted vote-based supervised analytical methods to find genes whose can classify samples presence or absence mesangial matrix expansion, best...
Chromosomal microarray analysis (CMA) has been used routinely in pediatric and prenatal genetic diagnosis clinical practice, but it rarely applied to miscarriage analysis. In this study, we conducted a prospective study evaluate the feasibility of CMA for first-trimester specimens. We successfully analyzed 551 fresh specimens using single-nucleotide polymorphism (SNP) array. Among specimens, 2.9% (16/551) had significant maternal cell contamination were excluded from study. Clinically...
Endometriosis is a common gynecological disease that affects approximately 5 %∼10 % of reproductive-aged women. Zinc (Zn), selenium (Se), copper (Cu), cobalt (Co) and molybdenum (Mo) are essential trace elements very important for human health. However, studies on the relationship between mixtures risk endometriosis limited inconsistent. In particular, confirming association via different sample types limited. This study aimed to investigate associations Zn, Se, Cu, Co Mo concentrations in...
Curcumin has been observed to exhibit an anti-fibrotic effect in the liver, lung and gallbladder. However, mechanisms underlying cytoprotective effects of curcumin remain be elucidated. The epithelial‑to‑mesenchymal transition (EMT) mature tubular epithelial cells kidney is considered contribute renal accumulation matrix proteins associated with diabetic nephropathy. EMT also closely progression interstitial fibrosis oxidative stress. This process may occur through abrogation high glucose...
The appearance and magnitude of the immune response related factors correlated with SARS-CoV-2 vaccination need to be defined. Here, we enrolled a prospective cohort 52 participants who received two doses inactivated vaccines (BBIBP-CorV). Their serial plasma samples (n = 260) over 2 months were collected at five timepoints. We measured antibody responses (NAb, S-IgG S-IgM) routine blood parameter. NAb seroconversion occurred in 90.7% vaccinated individuals four typical kinetic curves...
Range of DNA repair in response to double-strand breaks induced human preimplantation embryos remains uncertain due the complexity analyzing single- or few-cell samples. Sequencing such minute input requires a whole genome amplification that can introduce artifacts, including coverage nonuniformity, biases, and allelic dropouts at target site. We show here that, on average, 26.6% preexisting heterozygous loci control single blastomere samples appear as homozygous after indicative dropouts....
SARS-CoV-2 Omicron variants feature highly mutated spike proteins with extraordinary abilities in evading antibodies isolated earlier the pandemic. Investigation of memory B cells from patients primarily breakthrough infections Delta variant enables isolation a number neutralizing cross-reactive to heterologous concern (VOCs) including (BA.1-BA.4). Structural studies identify altered complementarity determining region (CDR) amino acids and unusual heavy chain CDR2 insertions respectively two...
The conversion of DNA 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC) by TET enzymes represents a significant epigenetic modification, yet its role in early human embryos remains largely unknown. Here we showed that the embryo inherited amount 5hmCs from an oocyte, which unexpectedly underwent de novo hydroxymethylation during growth. Furthermore, generation 5hmC paternal genome after fertilization roughly followed maternal pattern, was linked methylation dynamics and regions...
Reprogramming of somatic cells to induced pluripotent stem (iPSCs) shares much similarity the cancer initiation process, and molecular mechanisms underlying both processes remain be elucidated. Here, we report that a tumor- or embryonic cell-specific Ras gene ERas, which encodes constitutively active form GTPase, its downstream Phosphoinositide-3 kinase/Akt signaling pathway are important facilitators for reprogramming process. We found overexpression ERas retrovirally enhanced mouse iPSC...
Abstract Applications of genome editing ultimately depend on DNA repair triggered by targeted double-strand breaks (DSBs). However, mechanisms in human cells remain poorly understood and vary across different cell types. Here we report that DSBs selectively induced a mutant allele heterozygous embryos are repaired gene conversion using an intact wildtype homolog as template up to 40% embryos. We also show targeting homozygous loci facilitates interplay non-homologous end joining (NHEJ)...