A5079502883- Per- and polyfluoroalkyl substances research
- CRISPR and Genetic Engineering
- Effects and risks of endocrine disrupting chemicals
- Gestational Diabetes Research and Management
- Birth, Development, and Health
- RNA regulation and disease
- Monoclonal and Polyclonal Antibodies Research
- Biochemical Analysis and Sensing Techniques
- Toxic Organic Pollutants Impact
- Neurobiology and Insect Physiology Research
- T-cell and B-cell Immunology
- RNA and protein synthesis mechanisms
- Chemical Analysis and Environmental Impact
- Olfactory and Sensory Function Studies
- Immunotherapy and Immune Responses
- Pregnancy and Medication Impact
- Sexual Differentiation and Disorders
- Ovarian function and disorders
- Reproductive Biology and Fertility
- Antibiotic Use and Resistance
- Dark Matter and Cosmic Phenomena
- Maternal and Perinatal Health Interventions
- Breastfeeding Practices and Influences
- SARS-CoV-2 and COVID-19 Research
- melanin and skin pigmentation
Shanghai Center for Brain Science and Brain-Inspired Technology
2024
International Peace Maternity & Child Health Hospital
2021-2024
Shanghai Jiao Tong University
2015-2023
XinHua Hospital
2015-2023
ShanghaiTech University
2023
Center for Excellence in Brain Science and Intelligence Technology
2017-2022
Chinese Academy of Sciences
2002-2022
Shanghai Institutes for Biological Sciences
2017-2022
East China Normal University
2013-2019
Shanghai Blood Center
2019
The CRISPR/Cas9 system is an efficient gene-editing method, but the majority of gene-edited animals showed mosaicism, with editing occurring only in a portion cells. Here we show that single gene or multiple genes can be completely knocked out mouse and monkey embryos by zygotic injection Cas9 mRNA adjacent single-guide RNAs (spaced 10-200 bp apart) target key exon each gene. Phenotypic analysis F0 mice following targeted deletion eight on Y chromosome individually demonstrated robustness...
Epidemiologic evidence regarding the effects of in utero exposure to per- and polyfluoroalkyl substances (PFAS), particularly short-chain PFAS, on fetal reproductive hormones is limited inconsistent. This study aimed assess relationship between maternal PFAS hormones. A total 752 mother-infant pairs who were recruited Shanghai Birth Cohort Study 2013 2016 selected. We quantified 10 including two congeners (perfluorobutanesulfonate, PFBS perfluoroheptanoic acid, PFHpA), blood plasma early...
Humans are widely exposed to per- and polyfluoroalkyl substances (PFAS). As fetal stage is a critical window for neurodevelopment, it important know if in utero exposure PFAS affects neurodevelopment. However, previous human studies both limited inconsistent. To investigate the relationship between during early pregnancy neurodevelopmental status at 2 years of age prospective cohort study. We measured 10 maternal plasma samples collected prior 16 weeks gestation Shanghai Birth Cohort Study...
Abstract Transposon-associated ribonucleoprotein TnpB is known to be the ancestry endonuclease of diverse Cas12 effector proteins from type-V CRISPR system. Given its small size (408 aa), it interest examine whether engineered could used for efficient mammalian genome editing. Here, we showed that gene editing activity native Deinococcus radiodurans (ISDra2 TnpB) in mouse embryos was already higher than previously identified small-sized Cas12f1. Further stepwise engineering noncoding RNA...
Perfluoroalkyl substances (PFASs) were reported to be associated with hypertensive disorders of pregnancy (HDP) but the results inconsistent and prospective data are scarce. We aimed examine these associations in a large birth cohort study Shanghai, China.A total 10 PFASs measured by high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS-MS) plasma samples from 3220 women who enrolled during early gave singleton live between 2013 2016. The outcomes included gestational...
Perfluorobutanesulfonic acid (PFBS), a short-chain alternative to perfluorooctanesulfonic (PFOS), is widely used in various products and increasingly present environmental media human bodies. Recent epidemiological findings have raised concerns about its potential adverse health effects, although the specific toxic mechanism remains unclear. This study aimed investigate metabolic toxicity of gestational PFBS exposure maternal rats. Pregnant Sprague Dawley (SD) rats were randomly assigned...
Abstract Background Preterm birth (PTB, < 37 completed weeks’ gestation) is one of the global public health concerns. Epidemiologic evidence on potential impact perfluoroalkyl substances (PFAS) PTB still limited and inconsistent. We aimed to investigate associations between prenatal PFAS exposure among singleton live births. Methods studied 2849 mother-infant pairs in Shanghai Birth Cohort (SBC) from 2013 2016. Ten maternal plasma early pregnancy (gestational age, median (interquartile...
Emerging per- and polyfluoroalkyl substances (PFAS) alternatives are increasingly used in daily life. Although legacy PFAS have been associated with miscarriage previous studies, it remains unknown whether exposure to emerging has any impact on the risk of unexplained recurrent spontaneous abortion (URSA). We conducted a case-control study 464 URSA cases who had at least 2 miscarriages 440 normal controls one livebirth. Concentrations 21 plasma, including three alternatives, eight linear...
Triclosan (2,4,4'-trichloro-2'-hydroxy-diphenyl ether) is widely used in personal care and household products. Previous vitro vivo studies showed that triclosan may affect female reproductive health. However, evidence from human scarce.To assess the potential effects of on women's health.A prospective cohort study recruited 1,182 couples who planned to conceive presented preconception clinics for physical examination Shanghai, China, between 2013 2015. These were then prospectively followed...
Abstract STUDY QUESTION Is pre-conception exposure to parabens associated with fecundity in couples of childbearing age? SUMMARY ANSWER Paraben female partners was reduced couple and anti-Müllerian hormone (AMH) might be one the possible mediators. WHAT IS KNOWN ALREADY The reproductive toxicity parabens, a class widely used preservatives, has been suggested but evidence regarding their effects on is scarce. DESIGN, SIZE, DURATION In this couple-based prospective cohort study, total 884 who...
To investigate the potential therapeutic effect in a rheumatoid arthritis model of stable human CD8+ regulatory T cells (hCD8+Tregs) induced by TGF-β1 and rapamycin (RAPA) vitro. Human CD8+T were isolated from peripheral blood mononuclear induced/expanded with RAPA along anti-CD3/28 beads IL-2 vitro harvested as hCD8+Tregs. The phenotypes, suppressive characteristics, stability hCD8+Tregs an inflammatory microenvironment examined CD8+Tregs transfused into acollagen-induced (CIA) mouse model,...
Abstract Olfactory sensory neurons (OSNs) are functionally defined by their expression of a unique odorant receptor (OR). Mechanisms underlying singular OR well studied, and involve massive cross-chromosomal enhancer interaction network. Trace amine-associated receptors (TAARs) form distinct family olfactory receptors, here we find that mechanisms regulating Taar gene choice display many features. The epigenetic signature genes in TAAR OSNs is different from OSNs. We further identify two...
Data from the Tibet III air shower array (with energies around 3 TeV) and II 10 have been searched for diffuse gamma rays Galactic plane. These arrays an angular resolution of about 09. The sky regions are inner Galaxy, 20° ≤ l 55°, outer 140° 225°, |b| 2° or 5°. No significant Galactic-plane gamma-ray excess was observed. 99% confidence level upper limits intensity obtained (for 2°) 1.1 × 10-15 cm-2 s-1 sr-1 MeV-1 at TeV 4.1 10-17 3.6 10-16 1.3 assuming a differential spectral index 2.4. in...
Increasing evidence shows that antibiotic use in pregnancy may increase the risk of childhood asthma but epidemiologic studies are still limited and findings inconsistent. Meanwhile, exclusive prolonged breastfeeding prevent children from allergic diseases. We aimed to assess association between prenatal asthma, explore whether modifies risk.We conducted a case-control study Shanghai, China, June 2015 January 2016. A total 634 cases 864 controls aged 3-12 years were included. Multiple...