A5016295149- Cancer-related molecular mechanisms research
- Inflammatory Biomarkers in Disease Prognosis
- Advanced biosensing and bioanalysis techniques
- Extracellular vesicles in disease
- Peptidase Inhibition and Analysis
- Gastric Cancer Management and Outcomes
- Medication Adherence and Compliance
- Cancer Immunotherapy and Biomarkers
- Cancer, Lipids, and Metabolism
- Hemophilia Treatment and Research
- RNA modifications and cancer
- Cancer, Hypoxia, and Metabolism
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Diabetes Treatment and Management
- Ferroptosis and cancer prognosis
- RNA Interference and Gene Delivery
- Blood Coagulation and Thrombosis Mechanisms
- MicroRNA in disease regulation
- Macrophage Migration Inhibitory Factor
- Circular RNAs in diseases
- Platelet Disorders and Treatments
- Antiplatelet Therapy and Cardiovascular Diseases
- Cardiovascular Function and Risk Factors
- Complement system in diseases
- Galectins and Cancer Biology
Sun Yat-sen University
2016-2025
Sun Yat-sen University Cancer Center
2016-2025
Hospital Tuanku Ja’afar
2025
Takeda (United States)
2019-2024
Gunma University
2023
Synta Pharmaceuticals (United States)
2022
Guangzhou Medical University
2021
People’s Hospital of Wenshan Prefecture
2021
State Key Laboratory of Oncology in South China
2017-2020
Amgen (United States)
2019-2020
Tumor-derived extracellular vesicle (TEV) protein biomarkers facilitate cancer diagnosis and prognostic evaluations. However, the lack of reliable convenient quantitative methods for evaluating TEV proteins prevents their clinical application. Methods: Here, based on dual amplification hybridization chain reaction (HCR) CRISPR-Cas12a, we developed apta-HCR-CRISPR assay direct high-sensitivity detection proteins. The protein-targeted aptamer was amplified by HCR to produce a long-repeated...
MicroRNAs (miRNAs) show considerable promise as therapeutic agents to improve tumor treatment, they have been revealed crucial modulators in progression. However, our understanding of their roles gastric carcinoma (GC) metastasis is limited. Here, we aimed identify novel miRNAs involved GC and explored regulatory mechanisms significance GC.
Cancer-associated fibroblast assists cancer cell in building rampart to block T infiltration and resist anti–PD-1 drugs.
Epstein-Barr virus (EBV) infection has been observed in tumor-infiltrated macrophages, but its effects on macrophage immune functions are poorly understood. Here, we showed that some macrophages the tumor stroma of nasopharyngeal carcinoma (NPC) tissue expressed immunosuppressive protein indoleamine 2,3-dioxygenase (IDO) more strongly than did cells. EBV induced mRNA, protein, and enzymatic activity IDO human monocyte-derived (MDMs). Infection increased production necrosis factor alpha...
Predictive models for survival prediction in individual cancer patients following the tumor, node, and metastasis (TNM) staging system are limited.The rates of who share TNM stage diseases diversified.Therefore, we established a nomogram which hematological biomarkers clinical characteristics predicting overall (OS) nasopharyngeal carcinoma (NPC) were incorporated.The clinicopathological follow-up data 690 NPC histologically diagnosed at Sun Yat-sen University Cancer Center between July 2007...
Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia also vital the malignant behaviors of cancer cells. However, role its correlation not investigated HCC In tissues from post-surgical patients our center, we adopted immunohistochemistry staining (IHC), western blot quantitative RT-PCR to detect expression levels related marker, inducible factor...
Tumor-derived extracellular vesicles (TEVs) have emerged as promising sources of diagnostic and prognostic biomarkers for nasopharyngeal carcinoma (NPC). However, the lack high-sensitivity analytic methods ultratrace membrane proteins on TEVs hamper their clinical application TEVs. Herein, by combining aptamers that specifically bind to protein targets TEVs, PCR-based exponential amplification CRISPR/Cas12a real-time DNA detection, we developed a novel technique, termed aptamer-CRISPR/Cas12a...
Background: Currently, there are no molecular biomarkers for the early detection of non-small-cell lung cancer (NSCLC). This study focused on identifying RNAs found tumor-educated blood platelets (TEPs) detecting stage I NSCLC. Methods: Platelet RNAs, isolated from 9 patients with NSCLC (stages and II) 8 healthy controls, were analyzed using RNA-seq. ITGA2B was selected as a candidate marker. Two different Polymerase Chain Reactions (PCR) used to measure in platelet samples controls (n =...
Transcription factor Sp1 is multifaceted, with the ability to function as an oncogene or a tumor suppressor, depending on cellular context. We previously reported that required for transcriptional activation of key oncogenes in nasopharyngeal carcinoma (NPC), including B-lymphoma mouse Moloney leukemia virus insertion region 1 (Bmi1) and centromere protein H (CENPH), but role its underlying mechanisms NPC remained largely unexplored. The objective this study was investigate verify clinical...
Based on limited evidence, the U.S. Food and Drug Administration (FDA) issued a black box warning for use of tumor necrosis factor‐alpha inhibitors (TNFIs) risk non‐Hodgkin lymphoma (NHL). Our objective was to determine NHL associated with TNFI by duration type anti‐TNF agent. We performed nested case‐control study within retrospective cohort adults rheumatologic conditions from commercial health insurance database between 2009 2015. Use TNFIs (infliximab, adalimumab, etanercept, golimumab...
Multiple studies have reported the prognostic association of certain inflammatory factors with various types cancer. The present study assessed value C-reactive protein (CRP)/albumin (Alb) ratio and neutrophil/lymphocyte (NLR), separately in combination, gastric cancer (GC). A total 337 cases pathologically diagnosed adenocarcinoma were retrospectively evaluated. clinicopathological relevance CRP/Alb NLR their combination analyzed. optimal cut-off values 0.38 3.14, respectively. High (≥0.38)...
Behavior alterations in fibroblast-like synoviocytes (FLS) contribute to a pivotal role pathogenesis of rheumatoid arthritis (RA). MiRNAs are closely involved variety pathologic conditions. In the present study, we aimed screen for aberrant expression miRNAs (RA-FLS) further identify altered miR-26a-5p RA-FLS and investigate RA. The was screened by microarray analysis confirmed quantitative real time PCR. effect on proliferation, cell cycle, apoptosis, invasion were studied. verification...
The appearance and magnitude of the immune response related factors correlated with SARS-CoV-2 vaccination need to be defined. Here, we enrolled a prospective cohort 52 participants who received two doses inactivated vaccines (BBIBP-CorV). Their serial plasma samples (n = 260) over 2 months were collected at five timepoints. We measured antibody responses (NAb, S-IgG S-IgM) routine blood parameter. NAb seroconversion occurred in 90.7% vaccinated individuals four typical kinetic curves...
The absence of non-invasive biomarkers for the early diagnosis colorectal cancer (CRC) has contributed to poor prognosis. Extracellular vesicles (EVs) have emerged as promising candidates monitoring using liquid biopsy. However, complexity EVs isolation procedures and clear targets detecting serum-derived hindered clinical application in CRC diagnosis.
To evaluate whether serum Cathepsin S (Cat S) could serve as a biomarker for the diagnosis and prognosis of gastric cancer (GC), Enzyme-linked immuno sorbent assay (ELISA) was used to detect Cat in 496 participants including healthy controls patients with benign diseases, cancer, esophageal liver colorectal nasopharyngeal lung cancer. The levels were significantly increased patients, especially GC patients. qRT-PCR, Western blotting, immunohistochemical staining revealed overexpression cell...
B ‐lymphoma mouse Moloney leukemia virus insertion region 1 ( mi1), a member of the polycomb group, has elevated expression and is involved in pathogenesis various aggressive cancers, including nasopharyngeal carcinoma NPC ). To date, mechanisms underlying high mi1 remain obscure. gain new insights into transcriptional regulation BMI1 , we cloned characterized promoter . Luciferase reporter assays demonstrated that from −783 to +375 showed significant activity. With use series 5′‐deletion...
Elevated serum YKL-40 levels have been observed in various cancers. We evaluated the diagnostic performance of alone or combination with CEA, CYFRA21-1 and SCCA tumor markers for patients esophageal squamous cell carcinoma (ESCC). was detected ESCC lines tissues by real-time RT-PCR, Western blotting ELISA. protein expression determined 20 using immunohistochemistry. Serum measured ELISA 126 healthy donors, 59 benign diseases 150 ESCC. were electrochemiluminescence. mRNA cancer lines, culture...
Many patients with breast cancer are treated for other conditions and experience polypharmacy multiple concurrent medications. Our aim was to evaluate in relation adherence adjuvant endocrine therapy (AET) cancer.We conducted a retrospective cohort study of women incident, invasive initiating AET (tamoxifen, letrozole, anastrozole, exemestane) between 2009 2013 the Truven Health MarketScan Database. Polypharmacy pill burden were measured commonly used medications, including lipid-lowering...
Background. The pretreatment albumin and globulin ratio (AGR) was an inflammation-associated factor which related to the overall survival in various malignancies. aim of this study evaluate prognostic value AGR patients with gastric cancer. Method. This retrospective included 862 cases pathologically diagnosed All were randomly divided into testing group (431 cases) validation cases). relationships clinicopathologic characteristics prognosis analyzed by Kaplan-Meier Cox regression methods....
Abstract Programmed cell death receptor 1 (PD‐L1) protein on exosomes (exosomal PD‐L1) is one of the most promising biomarkers for cancer immunotherapy monitoring. However, current approaches exosomal PD‐L1 detection are poorly sensitive, laborious, and time‐consuming. Here, a new method, named Aptamer‐RPA‐TMA‐Cas13a Assay (ARTCA) established, which enables to be detected directly in serum with lower limit 10 particles mL −1 . Mechanistically, using DNA aptamer specifically binding PD‐L1,...
Abstract Plasmonic nanostructures offer an enticing prospect in many applications, ranging from lasing to biosensing, due their unrivaled light concentration beyond the diffraction limit. However, this promise is substantially undercut by intrinsically high losses metals. Here, experimental ultra‐high‐ Q plasmon resonance with a linewidth down 2 nm ( ‐factor ≈ 350) and intensity of 51% ultrasmooth gold nanogroove array reported. Such ultranarrow arises two key factors. First,...