A5008388188- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- Epigenetics and DNA Methylation
- Immune Cell Function and Interaction
- Reproductive Biology and Fertility
- Muscle Physiology and Disorders
- Monoclonal and Polyclonal Antibodies Research
- HIV Research and Treatment
- T-cell and B-cell Immunology
- Renal and related cancers
- Digital Holography and Microscopy
- Cardiomyopathy and Myosin Studies
- Advanced Optical Imaging Technologies
- Genetic Neurodegenerative Diseases
- Genetic Syndromes and Imprinting
- Advanced Image Processing Techniques
- SARS-CoV-2 and COVID-19 Research
- Phagocytosis and Immune Regulation
- Adipose Tissue and Metabolism
- RNA and protein synthesis mechanisms
- Image Processing Techniques and Applications
- Composting and Vermicomposting Techniques
- Orbital Angular Momentum in Optics
- Random lasers and scattering media
- interferon and immune responses
Center for Excellence in Molecular Cell Science
2013-2025
University of Chinese Academy of Sciences
2015-2025
Harbin University of Science and Technology
2023-2024
Ragon Institute of MGH, MIT and Harvard
2021-2024
Beijing University of Agriculture
2024
Massachusetts General Hospital
2024
Howard Hughes Medical Institute
2021-2022
Boston Children's Hospital
2021-2022
Chinese Academy of Sciences
2013-2017
Shanghai Institutes for Biological Sciences
2014-2015
Vaccines generate high-affinity antibodies by recruiting antigen-specific B cells to germinal centers (GCs), but the mechanisms governing recruitment GCs on secondary challenges remain unclear. Here, using preclinical SARS-CoV and HIV mouse models, we demonstrated that elicited during primary humoral responses shaped naive cell exposures. The from could either enhance or, conversely, restrict GC participation of cells: broad-binding, low-affinity, low-titer enhanced recruitment, whereas,...
Germline-targeting (GT) HIV vaccine strategies are predicated on deriving broadly neutralizing antibodies (bnAbs) through multiple boost immunogens. However, as the recruitment of memory B cells (MBCs) to germinal centers (GCs) is inefficient and may be derailed by serum antibody–induced epitope masking, driving further cell receptor (BCR) modification in GC-experienced after boosting poses a challenge. Using humanized immunoglobulin knockin mice, we found that GT protein trimer immunogen...
Broadly neutralizing antibodies (bnAbs) targeting the hemagglutinin (HA) stem of influenza A viruses (IAVs) tend to be effective against either group 1 or 2 viral diversity. In rarer cases, intergroup protective bnAbs can generated by human antibody paratopes that accommodate conserved glycan differences between and stems. We applied germline-engaging nanoparticle immunogens elicit a class cross-group from physiological precursor frequency within humanized mouse model. Cross-group protection...
Germline-targeting (GT) protein immunogens to induce VRC01-class broadly neutralizing antibodies (bnAbs) the CD4-binding site of HIV envelope (Env) have shown promise in clinical trials. Here, we preclinically validated a lipid nanoparticle-encapsulated nucleoside mRNA (mRNA-LNP) encoding eOD-GT8 60mer as soluble self-assembling nanoparticle mouse models. In model with three humanized B cell lineages bearing distinct VRC01-precursor receptors (BCRs) similar affinities for eOD-GT8, all could...
Placenta hyperplasia is commonly observed in cloned animals and believed to impede the proper development of embryos. However, mechanism underlying this phenomenon largely unknown. Here, we show that placenta mouse embryos occurs both middle late gestation. Interestingly, restoring paternal-specific expression an amino acid transporter Slc38a4, which loses maternal H3K27me3-dependent imprinting becomes biallelically expressed placentae, rescues overgrowth placentae at Molecular analyses...
How maternal factors in oocytes initiate zygotic genome activation (ZGA) remains elusive mammals, partly due to the challenge of de novo identification key using scarce materials. Two-cell (2C)-like cells have been widely used as an vitro model order understand mouse ZGA and totipotency because their expression a group two-cell embryo-specific genes simplicity for genetic manipulation. Recent studies indicate that DPPA2 DPPA4 are required establishing 2C-like state embryonic stem...
Abstract Multisystem manifestations in myotonic dystrophy type 1 (DM1) may be due to dosage reduction multiple genes induced by aberrant expansion of CTG repeats DMPK , including its neighboring ( SIX5 or DMWD ) and downstream MBNL1 . However, direct evidence is lacking. Here, we develop a new strategy generate mice carrying multigene heterozygous mutations mimic one step injection haploid embryonic stem cells with mutant Dmpk Six5 Mbnl1 into oocytes. The triple exhibit adult-onset DM1...
Growing evidence indicates the vital role of lipid metabolites in innate immunity. The lysophosphatidic acid (LPA) concentrations are enhanced patients upon HCV or SARS-CoV-2 infection, but function LPA and its receptors immunity is largely unknown. Here, we found that viral infection promoted G protein–coupled receptor LPA1 expression, restrained type I/III interferon production through LPA1. Mechanistically, signaling activated ROCK1/2, which phosphorylated IRF3 Ser97 to suppress...
In recent years, short-term composting techniques have been widely applied in oyster mushroom cultivation, but there is still a lack of systematic research on their impact the nutritional and functional properties fruiting bodies. this study, microbial inoculant Streptomyces thermoviolaceus BUA-FM01 (ST) was process for cultivation. The agronomic traits, composition, flavor compounds, antioxidant activity bodies from first three flushes were evaluated. results show that inoculation...
Abstract Genomic imprinting is required for sexual reproduction and embryonic development of mammals, in which, differentially methylated regions (DMRs) regulate the parent-specific monoallelic expression imprinted genes. Numerous studies on genes have highlighted their critical roles development. However, what network essential still unclear. Here, we establish a stepwise system to reconstruct development-related network, which diploid stem cells (ESCs) are derived by fusing between...
Understanding the mechanistic interplay between antibodies and invading pathogens is essential for vaccine development. Current methods are labour time intensive limited by sample preparation bottlenecks. Here we present microfluidic electron microscopy-based polyclonal epitope mapping (mEM), which combines microfluidics with single-particle microscopy structural characterization of immune complexes using small volumes sera (<4 µl). First, used mEM to map in from infected vaccinated...
Mammalian haploid embryonic stem cells (haESCs) provide new possibilities for large-scale genetic screens because they bear only one copy of each chromosome. However, haESCs are prone to spontaneous diploidization through unknown mechanisms. Here, we report that a small molecule combination could restrain mouse from by impeding exit naïve pluripotency and shortening the S-G2/M phases. Combined with 2i PD166285, our chemical cocktail maintain in state at least five weeks without...
Off-axis digital holography can reconstruct the amplitude and phase of imaging object by selecting original term spectrum through filtering. A larger filtering range contains more effective information from spectrum. However, presence zero-order limits size filter window consequently affects resolution reconstruction. In this work, we propose a spliced method based on binary Kronecker-product image interpolation to better suppress improve quality Additionally, rectangular Butterworth is also...
Abstract Multisystem manifestations in myotonic dystrophy type 1 (DM1) may be due to dosage reduction multiple genes induced by aberrant expansion of CTG repeats DMPK, including Dmpk and its neighboring (Six5 or Dmwd) downstream Mbnl1. However, the direct evidences are lack. Here, we develop a new strategy generate mice carrying multigene mutations one step injection haploid embryonic stem cells with mutant Dmpk, Six5 Mbnl1 into oocytes. The triple heterozygous exhibit adult-onset DM1...
ABSTRACT How maternal factors in oocytes initiate zygotic genome activation (ZGA) remains elusive. Recent studies indicate that DPPA2 and DPPA4 are required for establishing a 2-cell embryo-like (2C-like) state mouse embryonic stem cells (ESCs) DUX-dependent manner. These results suggest essential regulate Dux ZGA embryos. By analyzing knockout maternal-zygotic embryos, we unexpectedly found activation, ZGA, preimplantation development normal embryos without or DPPA4. Thus, unlike...
Wavefront distortion induced by scattering media seriously affects optical focusing. shaping based on a transmission matrix (TM) is useful in controlling light propagation highly media. Traditional TM generally studies amplitude and phase, but the stochastic nature of medium also its polarization. Based binary polarization modulation, we propose single (SPTM) achieve single-spot focusing through We anticipate that SPTM will be widely used wavefront shaping.
Phase retrieval algorithms, such as the Wirtinger Flow (WF) algorithm, are widely used in various fields. As a nonconvex optimization algorithm for phase retrieval, WF is commonly employed reconstruction of holograms holographic image projection. These types algorithms typically involve two stages: an initialization stage and iterative stage. In stage, initial value provided, spectral method to calculate approximate solution guess. The then utilizes gradient iteratively compute converge...