A5037223302- Plant Molecular Biology Research
- Chromosomal and Genetic Variations
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Plant Reproductive Biology
- Plant Disease Resistance and Genetics
- Genomics and Phylogenetic Studies
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- RNA modifications and cancer
- Genetic Mapping and Diversity in Plants and Animals
- Plant-Microbe Interactions and Immunity
- RNA and protein synthesis mechanisms
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Rice Cultivation and Yield Improvement
- Lipid metabolism and biosynthesis
- Metalloenzymes and iron-sulfur proteins
- Plant and Fungal Interactions Research
- Nematode management and characterization studies
- Plant responses to water stress
- Genetic and Environmental Crop Studies
- Genomics and Chromatin Dynamics
- Plant tissue culture and regeneration
Institute of Genetics and Developmental Biology
2016-2025
Chinese Academy of Sciences
2016-2025
State Key Laboratory of Plant Genomics
2014-2023
Center for Excellence in Molecular Plant Sciences
2016-2020
University of Chinese Academy of Sciences
2007-2019
Summary Higher plants have evolved multiple proteins in the RNase III family to produce and regulate different classes of small RNAs with specialized molecular functions. In rice ( Oryza sativa ), numerous genomic clusters are targeted by one two microRNAs (miRNAs), miR2118 miR2275, secondary interfering (siRNAs) either 21 or 24 nucleotides a phased manner. The biogenesis requirements functions completely unknown. Here we examine Dicer‐Like (DCL) family, including OsDCL1, ‐3a, ‐3b ‐4. By...
Significance The functional relationship of transposons and small RNAs remains an important question in the study gene expression its effect on agronomic traits. Here, we use deep sequencing to provide first evidence that rice Dicer-like 3 homolog OsDCL3a produces 24-nt interfering (siRNAs) predominantly associated with miniature inverted repeat transposable elements (MITEs). These siRNAs target genes adjacent MITEs act as broadly functioning regulators expression. In particular, directly...
Abstract Root-associated microbiomes contribute to plant growth and health, are dynamically affected by development changes in the soil environment. However, how different fertilizer regimes affect quantitative microbial assembly effect remains obscure. Here, we explore temporal dynamics of root-associated bacteria soybean using microbiome profiling (QMP) examine its response unbalanced treatments (i.e., lacking either N, P or K) role sustaining after four decades fertilization. We show that...
Abstract MicroRNAs and small interfering RNAs (siRNAs) are two classes of regulatory derived from different types precursors processed by distinct Dicer or Dicer-like (DCL) proteins. During evolution, four Arabidopsis thaliana DCLs six rice (Oryza sativa) (Os DCLs) appear to have acquired specialized functions. The well characterized, but those in remain largely unstudied. Here, we show that both knockdown loss function DCL4, the homolog lead vegetative growth abnormalities severe...
MicroRNAs (miRNAs) play key regulatory roles in numerous developmental and physiological processes animals plants. The elaborate mechanism of miRNA biogenesis involves transcription multiple processing steps. Here, we report the identification a pair evolutionarily conserved NOT2_3_5 domain-containing-proteins, NOT2a NOT2b (previously known as At-Negative on TATA less2 [NOT2] VIRE2-INTERACTING PROTEIN2, respectively), components involved Arabidopsis thaliana biogenesis. NOT2 was identified...
Hybrid rice is the dominant form of planted in China, and its use has extended worldwide since 1970s. It offers great yield advantages contributed greatly to world's food security. However, molecular mechanisms underlying heterosis have remained a mystery. In this study we integrated genetics omics analyses determine candidate genes for model two-line hybrid system, Liang-you-pei 9 (LYP9) parents. Phenomics revealed that better parent (BPH) not ascribed BPH all components but specific...
Transposable elements (TEs) are ubiquitously present in plant genomes and often account for significant fractions of the nuclear DNA. For example, roughly 40% rice genome consists TEs, many which retrotransposons, including 14% LTR- ∼1% non-LTR retrotransposons. Despite their wide distribution abundance, very few TEs have been found to be transpositional, indicating that TE activities may tightly controlled by host minimize potentially mutagenic effects associated with active transposition....
Higher plants have evolved multiple RNA-dependent RNA polymerases (RDRs), which work with Dicer-like (DCL) proteins to produce different classes of small RNAs specialized molecular functions. Here we report that OsRDR6, the rice (Oryza sativa L.) homolog Arabidopsis RDR6, acts in biogenesis various types and sizes RNAs. We isolated a osrdr6-1 mutant, was temperature sensitive showed spikelet defects. This mutant displays reduced accumulation tasiR-ARFs, conserved trans-acting siRNAs...
Abstract Heritable epigenetic variants of genes, termed epialleles, can broaden genetic and phenotypic diversity in eukaryotes. Epialleles may also provide a new source beneficial traits for crop breeding, but very few epialleles related to agricultural have been identified crops. Here, we Epi-rav6, gain-of-function epiallele rice (Oryza sativa) RELATED TO ABSCISIC ACID INSENSITIVE3 (ABI3)/VIVIPAROUS1 (VP1) 6 (RAV6), which encodes B3 DNA-binding domain-containing protein. The Epi-rav6 plants...
Abstract Recurrent heat stress and pathogen invasion seriously threaten crop production, abiotic often antagonizes biotic response against pathogens. However, the molecular mechanisms of trade-offs between thermotolerance defense remain obscure. Here, we identify a rice thermo-sensitive mutant that displays defect in floret development under high temperature with mutation S UPPRESSOR OF GENE SILENCING 3a ( OsSGS3a ). interacts its homolog OsSGS3b modulates biogenesis trans -acting small...
The two-line hybrid rice system, a cutting-edge breeding technology, has greatly boosted global food security. In thermo-sensitive genic male sterile (TGMS) lines, the critical sterility-inducing temperature (CSIT; at which TGMS lines change from fertile to complete sterile) acts as key threshold. We recently uncovered that sterility 5 ( tms5 ), locus presenting in over 95% of leads overaccumulation 2′,3′-cyclic phosphate (cP)-ΔCCA-tRNAs and deficiency mature tRNAs, underlies molecular...
In plants, microRNA (miRNA) functions in the post-transcriptional repression of target mRNAs have been well explored. However, mechanisms regulating accumulation miRNAs remain poorly understood. Here, we report that distinct regulate a monocot-specific miRNA, rice (Oryza sativa) miR528. At transcriptional level, miR528 accumulated to higher levels older plants than young seedlings and exhibited aging-modulated gradual diurnal rhythms leaves; at aging also modulated by enhancing pri-miR528...
Summary Ribosomal RNAs (rRNAs) undergo many modifications during transcription and maturation; homeostasis of rRNA is essential for chloroplast biogenesis in plants. The acts as a hub to sense environmental signals, such cold temperature. However, how RNA contribute low temperature responses remains unknown. Here we reveal that pseudouridine (Ψ) modification rice rRNAs mediated by the synthase (OsPUS1) contributes tolerance at seedling stage. Loss‐function OsPUS1 leads abnormal development...
Cold stress affects rice growth and productivity. Defects in the plastid-localized pseudouridine synthase OsPUS1 affect chloroplast ribosome biogenesis, leading to low-temperature albino seedlings accumulation of reactive oxygen species (ROS). Here, we report an ospus1-1 suppressor, sop10. SOP10 encodes a mitochondria-localized pentatricopeptide repeat protein. Mutations impair intron splicing nad4 nad5 transcripts decrease RNA editing efficiency nad2, nad6, rps4 transcripts, resulting...
Ribosome biogenesis is crucial for plant growth and environmental acclimation. Processing of ribosomal RNAs (rRNAs) an essential step in ribosome begins with transcription the rDNA. The resulting precursor-rRNA (pre-rRNA) transcript undergoes systematic processing, where multiple endonucleolytic exonucleolytic cleavages remove external internal transcribed spacers (ETS ITS). processing sites pathways pre-rRNA have been deciphered Saccharomyces cerevisiae and, to some extent, Xenopus laevis,...
, a dominant perennial grass in the Eurasian Steppe, is well known for its remarkable adaptability and forage quality. Hardly any breeding has been done on grass, limiting potential ecological restoration productivity. To enable genetic improvement of untapped, important species, we obtained 7.85-Gb high-quality genome
Abstract Low temperatures occurring at the booting stage in rice (Oryza sativa L.) often result yield loss by impeding male reproductive development. However, underlying mechanisms which responds to cold this remain largely unknown. Here, we identified MITOCHONDRIAL ACYL CARRIER PROTEIN 2 (OsMTACP2), encoded protein of mediates lipid metabolism involved response stage. Loss OsMTACP2 function compromised tolerance, hindering anther cuticle and pollen wall development, resulting abnormal...
Abstract Epigenetic regulation has been implicated in the control of complex agronomic traits rice (Oryza sativa), a staple food crop and model monocot plant. Recent advances high-throughput sequencing moderately genome have made it possible to study epigenetic on genome-wide scale. This review discusses recent our understanding rice, with an emphasis roles key regulators, epigenomic landscape, variation, transposon repression, plant development.