A5100353917- Plant-Microbe Interactions and Immunity
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Plant Parasitism and Resistance
- Plant Pathogenic Bacteria Studies
- Plant Virus Research Studies
- Legume Nitrogen Fixing Symbiosis
- Ubiquitin and proteasome pathways
- Plant pathogens and resistance mechanisms
- Plant Reproductive Biology
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Plant Physiology and Cultivation Studies
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Plant Pathogens and Fungal Diseases
- Immune Cell Function and Interaction
- Plant Disease Resistance and Genetics
- Plant Genetic and Mutation Studies
- Toxin Mechanisms and Immunotoxins
- RNA Research and Splicing
- Chromosomal and Genetic Variations
- Antimicrobial Peptides and Activities
- interferon and immune responses
- Cancer-related molecular mechanisms research
Guangdong Academy of Medical Sciences
2024-2025
Southern Medical University
2021-2025
Guangdong Provincial People's Hospital
2024-2025
Canada's Michael Smith Genome Sciences Centre
2016-2025
University of British Columbia
2016-2025
Guizhou University
2024-2025
Sichuan University
2021-2025
Shanghai University of Sport
2021-2025
McGill University
2024
Montreal Clinical Research Institute
2021-2024
The Arabidopsis thaliana NPR1 has been shown to be a key regulator of gene expression during the onset plant disease-resistance response known as systemic acquired resistance. npr1 mutant plants fail respond resistance-inducing signals such salicylic acid (SA), or express SA-induced pathogenesis-related ( PR ) genes. Using bait in yeast two-hybrid screen, we identified subclass transcription factors basic leucine zipper protein family (AHBP-1b and TGA6) showed that they interact specifically...
Salicylic acid (SA) is a plant defense hormone required for immunity. Arabidopsis NPR1 and NPR3/NPR4 were previously shown to bind SA all three proteins proposed as receptors. functions transcriptional co-activator, whereas suggested function E3 ligases that promote degradation. Here we report co-repressors inhibits their activities the expression of downstream immune regulators. npr4-4D, gain-of-function npr4 allele renders NPR4 unable SA, constitutively represses SA-induced responses. In...
The recently cloned NPR1 gene of Arabidopsis thaliana is a key regulator acquired resistance responses. Upon induction, expression elevated and the protein activated, in turn inducing battery downstream pathogenesis-related genes. In this study, we found that confers to pathogens Pseudomonas syringae Peronospora parasitica dosage-dependent fashion. Overexpression leads enhanced with no obvious detrimental effect on plants. Thus, for first time, single shown be workable target genetic...
Arabidopsis nonexpresser of pathogenesis-related (PR) genes (NPR1) is the sole positive regulator that has been shown to be essential for induction systemic acquired resistance. In npr1 mutant plants, salicylic acid (SA)-mediated PR gene expression and pathogen resistance are abolished completely. NPR1 interact with three closely related TGA transcription factors-TGA2, TGA5, TGA6-in yeast two-hybrid assays. To elucidate biological functions these factors, we analyzed single combined deletion...
Plants have evolved sophisticated defense mechanisms against pathogen infections, during which resistance (R) genes play central roles in recognizing pathogens and initiating cascades. Most of the cloned R share two common domains: domain, encodes a nucleotide binding adaptor shared by APAF-1, certain proteins, CED-4 (NB-ARC), plus C-terminal region that Leu-rich repeats (LRR). In Arabidopsis, dominant mutant, suppressor npr1-1, constitutive 1 (snc1), was identified previously constitutively...
Abstract The recognition of carbohydrate moieties by cells the innate immune system is emerging as an essential element in antifungal immunity, but despite number and diversity lectins expressed cells, few receptors have been characterized. Mincle, a C-type lectin, predominantly on macrophages, here shown to play role macrophage responses yeast Candida albicans. After exposure vitro, Mincle localized phagocytic cup, it was not for phagocytosis. In absence production TNF-α macrophages...
Salicylic acid (SA) is a defense hormone required for both local and systemic acquired resistance (SAR) in plants. Pathogen infections induce SA synthesis through up-regulating the expression of Isochorismate Synthase 1 ( ICS1 ), which encodes key enzyme production. Here we report that SAR Deficient (SARD1) CBP60g are regulators induction synthesis. Whereas knocking out SARD1 compromises basal SAR, overexpression constitutively activates responses. In sard1-1 cbp60g-1 double mutant,...
Abstract The function of PsBRC1, the pea (Pisum sativum) homolog maize (Zea mays) TEOSINTE BRANCHED1 and Arabidopsis (Arabidopsis thaliana) (AtBRC1) genes, was investigated. Psbrc1 mutant displays an increased shoot-branching phenotype, is able to synthesize strigolactone (SL), does not respond SL application. level pleiotropy SL-deficient ramosus1 (rms1) higher than in mutant, rms1 exhibiting a relatively dwarf phenotype more extensive branching at upper nodes. PsBRC1 gene mostly expressed...
Abstract Pathogen/microbe-associated molecular patterns (PAMPs/MAMPs) trigger plant immunity that forms the first line inducible defenses in plants. The regulatory mechanism of MAMP-triggered immunity, however, is poorly understood. Here, we show Arabidopsis thaliana transcription factors ETHYLENE INSENSITIVE3 (EIN3) and INSENSITIVE3-LIKE1 (EIL1), previously known to mediate ethylene signaling, also negatively regulate PAMP-triggered immunity. Plants lacking EIN3 EIL1 display enhanced PAMP...
The nucleotide-binding domain and leucine-rich repeats containing proteins (NLRs) serve as immune receptors in both plants animals. Overaccumulation of NLRs often leads to autoimmune responses, suggesting that the levels these must be tightly controlled. However, mechanism by which NLR protein are regulated is unknown. Here we report F-box CPR1 controls stability plant resistance proteins. Loss-of-function mutations lead higher accumulation SNC1 RPS2, well autoactivation responses. responses...
In Arabidopsis thaliana, the MEKK1-MKK1/MKK2-MPK4 mitogen-activated protein (MAP) kinase cascade represses cell death and immune responses. mekk1, mkk1 mkk2, mpk4 mutants, programmed defense responses are constitutively activated, but mechanism by which MEKK1, MKK1/MKK2, MPK4 negatively regulate immunity was unknown. From a screen for suppressors of we found that mutations in suppressor mkk2 1 (summ1) suppress not only also mekk1 mpk4. SUMM1 encodes MAP MEKK2. It interacts with is...
Summary Higher plants utilize nucleotide‐binding leucine‐rich repeat domain proteins ( NLR s) as intracellular immune receptors to recognize pathogen‐derived effectors and trigger a robust defense. The Activated Disease Resistance 1 ADR 1) family of coiled‐coil s CNL have evolved helper that function downstream many TIR ‐type sensor TNL s). Close homologs 1s form the N REQUIREMENT GENE NRG in Arabidopsis , which is unclear. Through CRISPR /Cas9 gene editing methods, we discovered tandemly...
The plant defense hormone salicylic acid (SA) is perceived by two classes of receptors, NPR1 and NPR3/NPR4. They function in parallel pathways to regulate SA-induced gene expression. To better understand the roles SA receptors defense, we systematically analyzed their contributions different aspects Arabidopsis (Arabidopsis thaliana) immunity using SA-insensitive npr1-1 npr4-4D double mutant. We found that perception NPR4 required for activation N-hydroxypipecolic biosynthesis, which...
NONEXPRESSOR OF PR GENES1 (NPR1) is a key regulator of the plant defense response known as systemic acquired resistance. Accumulation signal molecule salicylic acid (SA) leads to change in intracellular redox potential, enabling NPR1 enter nucleus and interact with TGACG sequence–specific binding protein (TGA) transcription factors, which turn bind SA-responsive elements promoters genes. Here, we show that two NPR1-like genes, BLADE-ON-PETIOLE1 (BOP1) BOP2, function redundantly control...
The Arabidopsis NPR1 protein is an essential regulatory component of systemic acquired resistance (SAR). Mutations in the gene completely block induction SAR by signals such as salicylic acid (SA). An mutant, snc1 (suppressor npr1-1, constitutive 1), was isolated a screen for suppressors npr1-1. In npr1-1 background, mutation resulted to Pseudomonas syringae maculicola ES4326 and Peronospora parasitica Noco2. High levels SA were detected mutant shown be required manifestation phenotype....