A5062629901- Advances in Cucurbitaceae Research
- Cocoa and Sweet Potato Agronomy
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Virus Research Studies
- Plant Pathogenic Bacteria Studies
- Plant tissue culture and regeneration
- Photosynthetic Processes and Mechanisms
- Phytoplasmas and Hemiptera pathogens
- Chromosomal and Genetic Variations
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Genetic Mapping and Diversity in Plants and Animals
- Plant Stress Responses and Tolerance
- Postharvest Quality and Shelf Life Management
- Plant responses to water stress
- Plant nutrient uptake and metabolism
- Silicon Effects in Agriculture
- Aluminum toxicity and tolerance in plants and animals
- Welding Techniques and Residual Stresses
- Rice Cultivation and Yield Improvement
- Polysaccharides and Plant Cell Walls
- Transgenic Plants and Applications
Shanghai Jiao Tong University
2013-2024
China University of Petroleum, Beijing
2024
Harbin Normal University
2023-2024
Nanchang University
2024
Northwest A&F University
2023
Wuhan University of Science and Technology
2022
Nanfang Hospital
2013
Shanghai Academy of Agricultural Sciences
2006-2009
Kansas State University
2007
Abstract The development of sensitive and versatile techniques to detect protein-protein interactions in vivo is important for understanding protein functions. previously described techniques, fluorescence resonance energy transfer bimolecular complementation, which are used widely interaction studies plants, require extensive instrumentation. To facilitate we adopted the luciferase complementation imaging assay. amino-terminal carboxyl-terminal halves firefly reconstitute active enzyme only...
Abstract Pathogen/microbe-associated molecular patterns (PAMPs/MAMPs) trigger plant immunity that forms the first line inducible defenses in plants. The regulatory mechanism of MAMP-triggered immunity, however, is poorly understood. Here, we show Arabidopsis thaliana transcription factors ETHYLENE INSENSITIVE3 (EIN3) and INSENSITIVE3-LIKE1 (EIL1), previously known to mediate ethylene signaling, also negatively regulate PAMP-triggered immunity. Plants lacking EIN3 EIL1 display enhanced PAMP...
Sex determination in plants involves a variety of mechanisms. Here, we report the map-based cloning and characterization unisexual-flower-controlling gene M. M was identified as previously characterized putative 1-aminocyclopropane-1-carboxylic acid synthase gene, while m allele that mutated at conserved site (Gly33Cys) lost activity original enzymatically active allele.
Structural variations (SVs) represent a major source of genetic diversity. However, the functional impact and formation mechanisms SVs in plant genomes remain largely unexplored. Here, we report nucleotide-resolution SV map cucumber (Cucumis sativas) that comprises 26,788 based on deep resequencing 115 diverse accessions. The largest proportion was formed through nonhomologous end-joining rearrangements, occurrence is closely associated with regions high nucleotide These affect coding 1676...
Powdery mildew (PM) is a serious fungal disease of cucumber worldwide. The identification resistance genes very important for breeding to ensure production. Here, natural loss-of-function mutations at an MLO homologous locus, CsMLO1, were found confer durable PM in cucumber. CsMLO1 encoded cell membrane protein, was mainly expressed leaves and cotyledons, up-regulated by the early stage host-pathogen interaction. Ectopic expression rescued phenotype resistant Atmlo2 Atmlo12 double mutant...
Abstract A maize genetic linkage map derived from 115 simple sequence repeat (SSR) markers was constructed an F 2 population. The generated a cross between stay‐green inbred line (Q319) and normal (Mo17). resolved 10 groups spanned 1431.0 cM in length with average distance of 12.44 two neighbouring loci. total 14 quantitative trait loci (QTL) were detected for traits at different postflowering time intervals identified by composite interval mapping. respective QTL contribution to phenotypic...
Summary Cucumber fruits that have tubercules and spines (trichomes) are known to possess a warty (Wty) phenotype. In this study, the tuberculate fruit gene Tu was identified by map‐based cloning, found encode transcription factor (TF) with single C 2 H zinc finger domain. in all 38 Wty lines examined, completely absent from 56 non‐warty ( nW ty) lines. plants transgenic for (TCP) revealed required Subcellular localization showed fusion protein GFP–Tu localized mainly nucleus. Based on...
It is well established that the plant hormone ethylene plays a key role in cucumber sex determination. Since unisexual control gene M was cloned and shown to encode an synthase, instead of receptor, 'one-hormone hypothesis', which used explain phenotype, has been challenged. Here, physiological function CsACS2 (the encoded by locus) studied using transgenic tobacco system. The results indicated overexpression increased production plant, native promoter had no activity (PM). However, when PM...
High-density genetic linkage map plays an important role in genome assembly and quantitative trait loci (QTL) fine mapping. Since the coming of next-generation sequencing, makes structure high-density maps much more convenient practical, which simplifies SNP discovery high-throughput genotyping. In this research, a cucumber was structured using specific length amplified fragment 153 F2 populations S1000 × S1002. The composed 3,057 SLAFs, including 4,475 markers on seven chromosomes, spanned...
The leaf is an important photosynthetic organ and plays essential role in the growth development of plants. Leaf color mutants are ideal materials for studying chlorophyll metabolism, chloroplast development, photosynthesis. In this study, we identified EMS-induced mutant, yl2.1, which exhibited yellow cotyledons true leaves that did not turn green with growth. yl2.1 locus was controlled by a recessive nuclear gene. CsYL2.1 mapped to 166.7-kb genomic region on chromosome 2, contains 24...
Abstract Unisexual flowers provide a useful system for studying plant sex determination. In cucumber (Cucumis sativus L.), three major Mendelian loci control unisexual flower development, Female (F), androecious [a; 1-aminocyclopropane-1-carboxylate {ACC} synthase 11, acs11], and Monoecious (M; ACS2), referred to here as the Female, Androecious, (FAM) model, in combination with two genes, gynoecious (g, WIP family C2H2 zinc finger transcription factor gene WIP1) ethylene biosynthetic ACC...
Abstract A 173‐point genetic linkage map of cucumber ( Cucumis sativus L.), consisting 116 SRAPs, 33 RAPDs, 11 SSRs, 9 SCARs, 3 ISSRs, and 1 STS, was constructed using 130 F 2 progeny derived from a narrow cross between line S94 (Northern China open‐field type) S06 (greenhouse European type). The seven groups spanned 1016 cM with mean marker interval 5.9 cM. Using the population its families, total 38 QTLs were detected on five an LOD threshold 3.0 for nine fruit‐related traits: fruit...
Waxy maize (Zea mays L. var. certaina Kulesh), with many excellent characters in terms of starch composition and economic value, has grown China for a long history its production increased dramatically recent decades. However, the evolution origin waxy still remains unclear. We studied genetic diversity Chinese including typical landraces inbred lines by SSR analysis results showed wide germplasm. analyzed sequencing 108 samples, downloading 52 sequences from GenBank locus number accessions...
In cucumber (Cucumis sativus L.), the differentiation and development of female flowers are important processes that directly affect fruit yield quality. Sex is mainly controlled by three ethylene synthase genes, F (CsACS1G), M (CsACS2) A (CsACS11). Thus, plays a key role in sex differentiation. The "one-hormone hypothesis" posits regulate levels initiate flower cucumber. Nonetheless, precise molecular mechanism this process remains elusive. To investigate which phenotype, near-isogenic...
TCP proteins are plant-specific transcription factors widely implicated in leaf morphogenesis and senescence, flowering, lateral branching, hormone crosstalk, stress responses. However, the relationship between pattern of TCPs organ development cucumber has not been systematically studied. In this study, we performed a genome-wide identification putative genes analyzed their chromosomal location, gene structure, conserved motif, transcript expression. A total 27 were identified characterized...
Cucumber is one of the most important vegetables in world. The C2H2 zinc finger protein (C2H2-ZFP) family plays an role growth development and abiotic stress responses plants. However, there have been no systematic studies on cucumber. In this study, we performed a genome-wide study C2H2-ZFP genes analyzed their chromosomal location, gene structure, conservation motif, transcriptional expression. total, 101 putative cucumber were identified divided into six groups (I-VI). RNA-seq...