A5084827613- Plant-Microbe Interactions and Immunity
- Plant Pathogenic Bacteria Studies
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- Plant Parasitism and Resistance
- Plant Disease Resistance and Genetics
- Plant Gene Expression Analysis
- Plant nutrient uptake and metabolism
- Plant tissue culture and regeneration
- Genetic Mapping and Diversity in Plants and Animals
- Microbial Applications in Construction Materials
- Chromosomal and Genetic Variations
- Cell Adhesion Molecules Research
- Electrospun Nanofibers in Biomedical Applications
- Plant biochemistry and biosynthesis
- Plant Virus Research Studies
- Genetics and Plant Breeding
- Plant Genetic and Mutation Studies
- Plant and animal studies
- RNA and protein synthesis mechanisms
- Plant pathogens and resistance mechanisms
- Monoclonal and Polyclonal Antibodies Research
South China Normal University
2016-2025
Anhui Agricultural University
2020-2025
Guangzhou University of Chinese Medicine
2024-2025
Shandong University of Traditional Chinese Medicine
2025
Peking University
2013-2024
Agricultural Genomics Institute at Shenzhen
2024
Chinese Academy of Agricultural Sciences
2024
Sichuan Agricultural University
2018-2024
Institute of Quality Standards and Testing Technology for Agro Products
2024
Nanjing Agricultural University
2005-2024
Abstract The development of sensitive and versatile techniques to detect protein-protein interactions in vivo is important for understanding protein functions. previously described techniques, fluorescence resonance energy transfer bimolecular complementation, which are used widely interaction studies plants, require extensive instrumentation. To facilitate we adopted the luciferase complementation imaging assay. amino-terminal carboxyl-terminal halves firefly reconstitute active enzyme only...
We report the complete genome sequence of model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on and Arabidopsis thaliana . The (6.5 megabases) contains a circular chromosome two plasmids, collectively encode 5,763 ORFs. identified 298 established putative virulence genes, including several clusters genes encoding 31 confirmed 19 predicted type III secretion system effector proteins. Many were members paralogous families also proximal to mobile...
Resistance to bacterial speck disease in tomato occurs when the Pto kinase plant responds expression of avirulence gene avrPto Pseudomonas pathogen. Transient an transgene cells containing elicited a defense response. In yeast two-hybrid system, physically interacted with AvrPto. Alterations AvrPto or that disrupted interaction also abolished resistance plants. The physical and provides explanation gene-for-gene specificity resistance.
Abstract Pathogen/microbe-associated molecular patterns (PAMPs/MAMPs) trigger plant immunity that forms the first line inducible defenses in plants. The regulatory mechanism of MAMP-triggered immunity, however, is poorly understood. Here, we show Arabidopsis thaliana transcription factors ETHYLENE INSENSITIVE3 (EIN3) and INSENSITIVE3-LIKE1 (EIL1), previously known to mediate ethylene signaling, also negatively regulate PAMP-triggered immunity. Plants lacking EIN3 EIL1 display enhanced PAMP...
A high-density single nucleotide polymorphism (SNP) array is critically important for geneticists and molecular breeders. With the accumulation of huge amounts genomic re-sequencing data available technologies accurate SNP detection, it possible to design high-quality rice arrays. Here we report development a its utility. probes were designed by screening more than 10 000 loci extracted from 801 varieties an named RiceSNP50 was produced on Illumina Infinium platform. The contained 51 478...
Significance Nuclear male sterility is common in flowering plants, but its application hybrid breeding and seed production limited because of the inability to propagate a pure sterile line for commercial production. Here, we characterized rice nuclear gene essential sporophytic fertility constructed system that can seeds on large scale. This fundamentally advantageous over current cytoplasmic photoperiod/thermo-sensitive genic systems. Application this technology will greatly enhance...
The ability of Pseudomonas syringae pv. tomato DC3000 to parasitize and Arabidopsis thaliana depends on genes activated by the HrpL alternative sigma factor. To support various functional genomic analyses DC3000, specifically, identify involved in pathogenesis, we developed a draft sequence used an iterative process involving computational gene expression techniques virulence-implicated downstream HrpL-responsive promoters. Hypersensitive response pathogenicity (Hrp) promoters are known...
The ability of Pseudomonas syringae pv. tomato DC3000 to be pathogenic on plants depends the Hrp (hypersensitive response and pathogenicity) type III protein secretion system effector proteins it translocates into plant cells. Through iterative application experimental computational techniques, inventory has been substantially enlarged. Five homologs known avirulence (Avr) five candidates, encoded by genes with putative promoters signatures horizontal acquisition, were demonstrated secreted...
Arabidopsis NONHOST1 ( NHO1 ) is required for limiting the in planta growth of nonhost Pseudomonas bacteria but completely ineffective against virulent bacterium syringae pv. tomato DC3000. However, molecular basis underlying this observation remains unknown. Here we show that transcriptionally activated by flagellin. The P. tabaci lacking flagellin unable to induce , multiplies much better than does wild-type bacterium, and causes disease symptoms on . DC3000 also possesses potent...
The tomato disease resistance (R) gene Pto specifies race-specific to the bacterial pathogen Pseudomonas syringae pv carrying avrPto gene. encodes a serine/threonine protein kinase that is postulated be activated by physical interaction with AvrPto protein. Here, we report overexpression of in activates defense responses absence Pto–AvrPto interaction. Leaves three transgenic lines cauliflower mosaic virus 35S::Pto transgene exhibited microscopic cell death, salicylic acid accumulation, and...
Abstract The successful recognition of pathogen-associated molecular patterns (PAMPs) as a danger signal is crucial for plants to fend off numerous potential pathogenic microbes. relayed through mitogen-activated protein kinase (MPK) cascades activate defenses. Here, we show that the Pseudomonas syringae type III effector HopF2 can interact with Arabidopsis thaliana MAP KINASE KINASE5 (MKK5) and likely other MKKs inhibit MPKs PAMP-triggered immunity. Inhibition PAMP-induced MPK...
Bactrian camels serve as an important means of transportation in the cold desert regions China and Mongolia. Here we present a 2.01 Gb draft genome sequence from both wild domestic bactrian camel. We estimate camel to be 2.38 Gb, containing 20,821 protein-coding genes. Our phylogenomics analysis reveals that shared common ancestors with other even-toed ungulates about 55–60 million years ago. Rapidly evolving genes lineage are significantly enriched metabolic pathways, these changes may...
The avrPto gene of Pseudomonas syringae pv tomato triggers race-specific resistance in plants carrying Pto, a encoding protein kinase. When introduced into P. s. tabaci, tobacco W38 that carry the corresponding R gene. AvrPto is believed to be secreted host cells through bacterial type III secretion pathway, where it activates disease by interacting with Pto. We report here identification two distinct regions determine recognition specificity this and tobacco. Point mutations central region...
It is poorly understood why a particular plant species resistant to the vast majority of potential pathogens that infect other species, phenomenon referred as “nonhost” resistance. Here, we show Arabidopsis NHO1 , encoding glycerol kinase, required for resistance and induced by Pseudomonas syringae isolates from bean tobacco. also fungal pathogen Botrytis cinerea indicating not limited bacterial Strikingly, P. s. pv. tomato DC3000, an isolate fully virulent on actively suppressed expression....
Summary Gram‐negative bacteria use a variety of virulence factors including phytotoxins, exopolysaccharides, effectors secreted by the type III secretion system, and cell‐wall‐degrading enzymes to promote parasitism in plants. However, little is known about how these alter plant cellular responses disease. In this study, we show that virulent Pseudomonas syringae strains activate transcription an Arabidopsis ethylene response factor ( ERF ) gene, RAP2.6 , coronatine insensitive 1...
Nonhost interactions are prevalent between plants and specialized phytopathogens. Although it has great potential for providing crop with durable resistance, nonhost resistance is poorly understood. Here, we show that controlled, at least in part, by general resistance. Arabidopsis resistant to the pathogen Pseudomonas syringae pv phaseolicola NPS3121 completely arrest bacterial multiplication plant. Ten mutants were isolated compromised (nho) P. s. phaseolicola. Among these, nho1 caused a...
The differential expression of the petunia 1-aminocyclopropane-1-carboxylate (ACC) oxidase gene family during flower development and senescence was investigated. ACC catalyzes conversion to ethylene. increase in ethylene production by corollas preceded increased mRNA enzyme activity. Treatment flowers with led an production, mRNA, activity corollas. In contrast, leaves did not exhibit or response Gene-specific probes revealed that ACO1 expressed specifically senescing other floral organs...
Summary The central importance of protein phosphorylation in plant defense responses has been demonstrated by the isolation several disease‐resistance genes that encode kinases. In addition, there are many reports changes accompanying to pathogens. contrast, little is known about role dephosphorylation regulating defenses. We report expression LePP2Ac1 gene, which encodes a catalytic subunit heterotrimeric phosphatase 2A (PP2Ac), rapidly induced resistant tomato leaves upon inoculation with...
Pathogenic bacterial effectors suppress pathogen-associated molecular pattern (PAMP)-triggered host immunity, thereby promoting parasitism. In the presence of cognate resistance genes, it is proposed that plants detect virulence activity and trigger a defense response, referred to here as effector-triggered immunity (ETI). However, link between effector ETI at level unknown. Here, we show Pseudomonas syringae AvrB suppresses PAMP-triggered (PTI) through RAR1, co-chaperone HSP90 required for...