A5100381049- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Microtubule and mitosis dynamics
- Plant-Microbe Interactions and Immunity
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Plant Pathogenic Bacteria Studies
- Mast cells and histamine
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Glycosylation and Glycoproteins Research
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Legume Nitrogen Fixing Symbiosis
- Asthma and respiratory diseases
- Cellular transport and secretion
- Pasture and Agricultural Systems
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Cellular Mechanics and Interactions
- Plant Ecology and Soil Science
- Invertebrate Immune Response Mechanisms
Duke Medical Center
2008-2025
Duke University Hospital
2005-2025
Huazhong University of Science and Technology
2024
Central Hospital of Wuhan
2024
Jiangnan University
2012-2024
Harbin Veterinary Research Institute
2023
Chinese Academy of Agricultural Sciences
2023
Xi'an University of Science and Technology
2023
Chinese Academy of Medical Sciences & Peking Union Medical College
2021
Suzhou Institute of Systems Medicine
2021
Abstract Jasmonates play a number of diverse roles in plant defense and development. CORONATINE INSENSITIVE1 (COI1), an F-box protein essential for all the jasmonate responses, interacts with multiple proteins to form SCFCOI1 E3 ubiquitin ligase complex recruits ZIM-domain (JAZ) degradation by 26S proteasome. To determine which directly binds jasmonoyl-isoleucine (JA-Ile)/coronatine (COR) serves as receptor jasmonate, we built high-quality structural model COI1 performed molecular modeling...
The mammalian target of rapamycin (mTOR) is centrally involved in cell growth, metabolism, and angiogenesis. While showing clinical efficacy a subset tumors, rapalogs are specific allosteric inhibitors mTOR complex 1 (mTORC1), but they do not directly inhibit 2 (mTORC2), an emerging player cancer. Here, we report chemical structure biological characterization three pyrazolopyrimidine ATP-competitive inhibitors, WAY-600, WYE-687, WYE-354 (IC(50), 5-9 nmol/L), with significant selectivity over...
Surveillance of the extracellular environment by immune receptors is central importance to eukaryotic survival. The rice receptor kinase XA21, which confers robust resistance most strains Gram-negative bacterium Xanthomonas oryzae pv. (Xoo), representative a large class cell surface in plants and animals. We report identification previously undescribed Xoo protein, called RaxX, required for activation XA21-mediated immunity. that lack or carry mutations single RaxX tyrosine residue (Y41),...
Abstract Effective plant defense strategies rely in part on the perception of non-self determinants, so-called microbe-associated molecular patterns (MAMPs), by transmembrane pattern recognition receptors leading to MAMP-triggered immunity. Plant resistance against necrotrophic pathogens with a broad host range is complex and yet not well understood. Particularly, it unclear if necrotrophs involves receptors. Here, we partially purified novel proteinaceous elicitor called SCLEROTINIA CULTURE...
Arabidopsis NONHOST1 ( NHO1 ) is required for limiting the in planta growth of nonhost Pseudomonas bacteria but completely ineffective against virulent bacterium syringae pv. tomato DC3000. However, molecular basis underlying this observation remains unknown. Here we show that transcriptionally activated by flagellin. The P. tabaci lacking flagellin unable to induce , multiplies much better than does wild-type bacterium, and causes disease symptoms on . DC3000 also possesses potent...
Centromeres are the structural and functional foundation for kinetochore formation, spindle attachment, chromosome segregation. In this study, we isolated factors required centromere propagation using genome-wide RNA interference screening defects in protein A (CENP-A; identifier [CID]) localization Drosophila melanogaster. We identified proteins CAL1 CENP-C as essential CID assembly at centromere. CID, CAL1, coimmunoprecipitate mutually dependent function. also mitotic cyclin (CYCA)...
In this study, we show that a reduction in the levels of JIL-1 histone H3S10 kinase results spreading major heterochromatin markers dimethyl H3K9 and HP1 to ectopic locations on chromosome arms, with most pronounced increase X chromosomes. Genetic interaction assays demonstrated functions vivo pathway includes Su(var)3-9, which is catalyst for dimethylation residue, recruitment, formation silenced heterochromatin. We further provide evidence activity localization are not affected by absence...
Allergen Affinity Allergic responses are initiated by interaction of allergens with immunoglobulin E (IgE) antibodies, which in turn bind to IgE receptors on the surface mast cells. Suzuki et al. (p. 1021 , published online 6 February; see Perspective Daëron ) explored how signaling properties such receptor complexes differ, depending affinity antibody for antigen leading different allergic responses.
The biotrophic pathogen Xanthomonas oryzae pv. (Xoo) produces a sulfated peptide named RaxX, which shares similarity to peptides in the PSY (plant containing tyrosine) family. We hypothesize that RaxX mimics growth-stimulating activity of peptides. Root length was measured Arabidopsis and rice treated with synthetic also used comparative genomic analyses reactive oxygen species burst assays evaluate Here we found derivative comprising 13 residues (RaxX13-sY), highly conserved between PSY,...
The centromere regulates proper chromosome segregation, and its dysfunction is implicated in chromosomal instability (CIN). However, relatively little known about how occurs cancer. Here, we define the consequences of phosphorylation by cyclin E1/CDK2 on a conserved Ser18 residue centromere-associated protein CENP-A, an essential histone H3 variant that specifies identity. hyperphosphorylation cells occurred upon loss FBW7, tumor suppressor whose inactivation leads to CIN. This event CENP-A...
Linker for activation of B cells (LAB, also called NTAL; a product wbscr5 gene) is newly identified transmembrane adaptor protein that expressed in cells, NK and mast cells. Upon BCR activation, LAB phosphorylated interacts with Grb2. capable rescuing thymocyte development LAT-deficient mice. To study the vivo function LAB, LAB-deficient mice were generated. Although disruption Lab gene did not affect lymphocyte development, it caused to be hyperresponsive stimulation via FcεRI, evidenced by...
Linker for activation of T cells (LAT) is an adaptor protein whose tyrosine phosphorylation critical transduction the cell receptor (TCR) signal. LAT accomplished by kinase ZAP-70, but it not at all clear how (which associated with TCR) encounters ZAP-70 bound to TCR). Here we show that associates surface CD4 and CD8 coreceptors its association promoted same coreceptor cysteine motif mediates Lck binding. In fact, competes binding individual molecules differs from in preferential rather than...
Abstract Linker for activation of T cells (LAT) is a membrane-associated adaptor protein that phosphorylated on multiple tyrosines upon TCR cross-linking. Previous studies show LAT essential TCR-mediated signaling and thymocyte development. In this study, we expressed series Tyr to Phe mutants in LAT-deficient J.CaM2.5 examined their tyrosine phosphorylation; association with Grb2, Gads, phospholipase C (PLC)-γ1; function cell activation. Our results showed the five membrane-distal were...