A5043396737- Gut microbiota and health
- Clostridium difficile and Clostridium perfringens research
- Probiotics and Fermented Foods
- Genomics and Phylogenetic Studies
- Autism Spectrum Disorder Research
- Aquaculture disease management and microbiota
- SARS-CoV-2 and COVID-19 Research
- Genetics and Neurodevelopmental Disorders
- Advanced biosensing and bioanalysis techniques
- Antimicrobial Peptides and Activities
- Vibrio bacteria research studies
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- COVID-19 Clinical Research Studies
- Diet and metabolism studies
- Genetic Mapping and Diversity in Plants and Animals
- Genomic variations and chromosomal abnormalities
- Antenna Design and Analysis
- Nanoplatforms for cancer theranostics
- Nanoparticle-Based Drug Delivery
- Genetic and phenotypic traits in livestock
- Animal Virus Infections Studies
- Metabolomics and Mass Spectrometry Studies
- Chromosomal and Genetic Variations
- Infant Nutrition and Health
Peking University
2025
Zhejiang A & F University
2024-2025
Ocean University of China
2025
Harvard University
2018-2024
Henan University of Traditional Chinese Medicine
2024
Sun Yat-sen University
2020-2024
Shanxi University
2024
Shanxi Academy of Agricultural Sciences
2024
Lanzhou Jiaotong University
2024
Tianjin Medical University General Hospital
2024
The probiotic bacterium Phaeobacter inhibens strain S4Sm, isolated from the inner shell surface of a healthy oyster, secretes antibiotic tropodithietic acid (TDA), is an excellent biofilm former, and increases oyster larvae survival when challenged with bacterial pathogens. In this study, we investigated specific roles TDA secretion formation in activity S4Sm.Mutations clpX (ATP-dependent ATPase) exoP (an exopolysaccharide biosynthesis gene) were created by insertional mutagenesis using...
Metagenome of gut microbes has been implicated in metabolism, immunity, and health maintenance its host. However, most previous studies, the microbiota was sampled from feces instead gastrointestinal (GI) tract. In this study, we compared microbial populations at four different developmental stages contents intestinal segments maturity to examine dynamic shift pigs investigated whether adult porcine fecal samples could be used represent GI Analysis results revealed that ratio Firmicutes...
Abstract Recurrent de novo (DN) and likely gene-disruptive (LGD) mutations contribute significantly to autism spectrum disorders (ASDs) but have been primarily investigated in European cohorts. Here, we sequence 189 risk genes 1,543 Chinese ASD probands (1,045 from trios). We report an 11-fold increase the odds of DN LGD compared with expectation under exome-wide neutral model mutation. In aggregate, ∼4% patients carry a mutation one just 29 genes. The most prevalent gene for recurrent is...
Host genotype and gender are among the factors that influence composition of gut microbiota. We studied population structure microbiota in two lines chickens maintained under same husbandry dietary regimes. The lines, which originated from a common founder population, had undergone 54 generations selection for high (HW) or low (LW) 56-day body weight now differ by more than 10-fold at age. Of 190 microbiome species, 68 were affected (line), interactions. Fifteen species belong to...
In mammals, the microbiota can be transmitted from placenta, uterus, and vagina of mother to infant. Unlike development avian embryo is a process isolated thus in gut microbial developmental remains elusive. To explore establishment inheritance microbiome embryo, we used chicken as model organism investigate composition embryos, chicks maternal hens. We observed: (1) 28 phyla 162 genera microbes embryos where dominated genus was Halomonas (79%). (2) 65 were core all stages with 42% 62% found...
Cholera pathogen zaps competition Many bacterial pathogens inject their hosts with virulence effectors delivered by specialist secretion machines. Vibrio cholerae has a type VI system (T6SS) that can be loaded protein toxins target eukaryote host cells or kill competing bacteria. Zhao et al. discovered mutant V. lacking T6SS could not compete against Escherichia coli strains in the mouse gut. In contrast, intact readily gained foothold gut of young mice, pumping up inflammatory immune...
Bactrian camels serve as an important means of transportation in the cold desert regions China and Mongolia. Here we present a 2.01 Gb draft genome sequence from both wild domestic bactrian camel. We estimate camel to be 2.38 Gb, containing 20,821 protein-coding genes. Our phylogenomics analysis reveals that shared common ancestors with other even-toed ungulates about 55–60 million years ago. Rapidly evolving genes lineage are significantly enriched metabolic pathways, these changes may...
We previously performed targeted sequencing of autism risk genes in probands from the Autism Clinical and Genetic Resources China (ACGC) (phase I). Here, we expand this analysis to a larger cohort patients (ACGC phase II) better understand prevalence, inheritance, genotype-phenotype correlations likely gene-disrupting (LGD) mutations for candidate originally identified cohorts European descent.We sequenced 187 an additional 784 85 599 using single-molecule molecular inversion probes. tested...
The homeostasis of host-microbiota interactions is great importance to host health. Previous studies demonstrated that disruption autophagy was linked inflammatory bowel disease. However, the interaction mechanism gut microbiota regulated by obscure. In an intestinal epithelium-specific autophagy-related 5 ( Atg5 ) knockout mouse model, we observed a significant alteration and decreased diversity in -deficient mice compared with wild-type mice. Although numbers some organisms (e.g.,...
Abstract B cell response plays a critical role against SARS-CoV-2 infection. However, little is known about the diversity and frequency of paired antigen-specific BCR repertoire after Here, we performed single-cell RNA sequencing VDJ using memory plasma cells isolated from five convalescent COVID-19 patients, analyzed spectrum transcriptional heterogeneity antibody immune responses. Via linking to antigen specificity through (LIBRA-seq), identified distinct activated subgroup ( CD11c high...
SARS-CoV-2, the causative agent for COVID-19, infect human mainly via respiratory tract, which is heavily inhabited by local microbiota. However, interaction between SARS-CoV-2 and nasopharyngeal microbiota, association with metabolome has not been well characterized. Here, metabolomic analysis of blood, urine, swabs from a group COVID-19 non-COVID-19 patients, metagenomic pharyngeal samples were used to identify key features COVID-19. Results showed lactic acid, l-proline, chlorogenic acid...
Nonalcoholic fatty liver disease (NAFLD) usually includes NAFL called simple hepatosteatosis and nonalcoholic steatohepatitis (NASH) more steatohepatitis. The latter is a leading pathogenic promotor of hepatocellular carcinoma (HCC). Phytochemical gallic acid (GA) has been proved to exert positive efficacy in HCC our work, but it remains unclear whether its hepatoprotective effect attributes the controlled transition from steatosis steatohepatitis.This work aims provide mechanistic evidence...
Anti-PD-1 immunotherapy has saved numerous lives of cancer patients; however, it only exerts efficacy in 10-15% patients with colorectal cancer. Fecal microbiota transplantation (FMT) is a potential approach to improving the anti-PD-1 therapy, whereas detailed mechanisms and applicability this combination therapy remain unclear. In study, we evaluated synergistic effect FMT curing tumor-bearing mice using multi-omics approach. Mice treated showed superior survival rate tumor control,...
The emergence of multidrug-resistant bacterial pathogens is a growing threat to global public health. Here, we report the development and characterization panel nine–amino acid residue synthetic peptides that display potent antibacterial activity ability disrupt preestablished microbial biofilms. lead peptide (Peptide K6) showed bactericidal against Pseudomonas aeruginosa Staphylococcus aureus in culture monocultures mixed biofilms vitro. Biophysical analysis revealed Peptide K6...
Karyotypic diversification is more prominent in Equus species than other mammals. Here, using next generation sequencing technology, we generated and de novo assembled quality genomes sequences for a male wild horse (Przewalski's horse) domestic (Mongolian horse), with about 93-fold 91-fold coverage, respectively. Portion of Y chromosome from assemblies (3 M bp) Mongolian (2 were also sequenced assembled. We confirmed Robertsonian translocation event through the horse's chromosomes 23 24,...
Bacterial pathogens, including several Vibrio spp. and Roseovarius crassostreae, cause severe mortality of larval juvenile eastern oysters. The introduction beneficial bacterial isolates in oyster hatcheries nurseries for the biocontrol diseases is a good alternative to use antibiotics. goal this study was screen characterize marine as potential agents prevent by pathogens tubiashii R. crassostreae. Screening from Rhode Island organisms environment using agar-based assay methods detection...
The abundance of gut microbiota can be viewed as a quantitative trait, which is affected by the genetics and environment host. To quantify effects host genetics, we calculated heritability specific microorganisms genetic correlations among them in two lines chickens maintained under same husbandry dietary regimes. lines, originated from common founder population, had undergone >50 generations selection for high (HW) or low (LW) 56-day body weight now differ more than 10-fold at age. We...
The gut microbiota is associated with cardiovascular diseases, including atherosclerosis. However, the composition, functional capacity, and metabolites of microbiome about atherosclerosis have not been comprehensively studied. Here, we reanalyzed 25 metagenomic stool samples from Sweden 385 China using HUMAnN2, PanPhlAn, MelonnPan to obtain more sufficient information. We found that atherosclerotic patients in both cohorts were depleted Bacteroides xylanisolvens, Odoribacter splanchnicus,...
The potentially beneficial effects of probiotics in the treatment obesity have been generally demonstrated. In present study, a new strain