A5009819342- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Phytoplasmas and Hemiptera pathogens
- Cocoa and Sweet Potato Agronomy
- Plant Pathogens and Fungal Diseases
- Bacterial Genetics and Biotechnology
- Plant Parasitism and Resistance
- Plant tissue culture and regeneration
- Bacteriophages and microbial interactions
- Plant pathogens and resistance mechanisms
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Banana Cultivation and Research
- Plant Virus Research Studies
- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Plant nutrient uptake and metabolism
- Molecular Biology Techniques and Applications
- Plant Molecular Biology Research
- Biochemical and Molecular Research
- RNA modifications and cancer
- Microbial Metabolism and Applications
- Pineapple and bromelain studies
National Chung Hsing University
2013-2023
National Taipei University
2010-2021
Academia Sinica
2020-2021
Sichuan Agricultural University
2020
Cornell University
1998-2003
United States Department of Agriculture
2000-2003
University of Nebraska–Lincoln
2003
University of Nevada, Las Vegas
2000
Michigan State University
2000
Western Regional Research Center
2000
We report the complete genome sequence of model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on and Arabidopsis thaliana . The (6.5 megabases) contains a circular chromosome two plasmids, collectively encode 5,763 ORFs. identified 298 established putative virulence genes, including several clusters genes encoding 31 confirmed 19 predicted type III secretion system effector proteins. Many were members paralogous families also proximal to mobile...
ABSTRACT We determined the complete genome sequence of Shigella flexneri serotype 2a strain 2457T (4,599,354 bp). species cause >1 million deaths per year from dysentery and diarrhea have a lifestyle that is markedly different those closely related bacteria, including Escherichia coli . The exhibits backbone island mosaic structure E. pathogens, albeit with much less horizontally transferred DNA lacking 357 genes present in distinctive its large complement insertion sequences, several...
The plant pathogenic bacterium Pseudomonas syringae is divided into pathovars differing in host specificity, with P. pv. ( Psy ) and tomato Pto representing particularly divergent pathovars. hrp / hrc genes encode a type III protein secretion system that appears to translocate Avr Hop effector proteins cells. DNA sequence analysis of the regions 61, B728a, DC3000 has revealed Hrp pathogenicity island (Pai) tripartite mosaic structure. gene cluster conserved all three strains flanked by...
Erwinia chrysanthemi is representative of a broad class bacterial pathogens that are capable inducing necrosis in plants. The E. EC16 hecA gene predicts 3,850-aa member the Bordetella pertussis filamentous hemagglutinin family adhesins. A ∷Tn 7 mutant was reduced virulence on Nicotiana clevelandii seedlings after inoculation without wounding. Epifluorescence and confocal laser-scanning microscopy observations wild-type cells expressing green fluorescent protein revealed its ability to attach...
Bacterial surface appendages called pili often are associated with DNA and/or protein transfer between cells. The exact function of in the process is not understood and a matter considerable debate. Hrp pilus assembled by type III secretion system Pseudomonas syringae pv. tomato ( Pst ) strain DC3000. In this study, we show that hrpA gene, which encodes major subunit pilus, required for putative virulence proteins, such as HrpW AvrPto. addition, gene full expression genes encode regulatory,...
Global warming and climate change have contributed to the rise of weather extremes. Severe drought soil salinization increase because rising temperatures. Economically important crop production plant growth development are hindered when facing various abiotic stresses. Plant endophytic bacteria live inside host plants without causing visible harm can be isolated from surface-sterilized tissues. Using stimulate environmental stress tolerance has become an alternative approach besides using...
Mutations in the five hrp and hrc genes hrpC operon of phytopathogen Pseudomonas syringae pv. 61 have different effects on bacterial interactions with host nonhost plants. The hrcC gene within encodes an outer membrane component Hrp secretion system that is conserved all type III protein systems required for most pathogenic phenotypes HrpZ harpin to milieu. other four (in order), hrpF, hrpG, (hrcC), hrpT, hrpV, appear be unique group I clusters found certain phytopathogens (e.g., P. Erwinia...
Pseudomonas syringae pv. 61 contains a 25-kb hrp cluster that is sufficient to elicit the hypersensitive response (HR) in nonhost plants. Previous studies have shown mutations complementation groups VIII, IX, and XI abolished ability of bacterium cause HR. The sequence 3.7-kb SmaI-SstI fragment covering VIII IX now reveals five open reading frames (ORFs) same transcript, designated as hrpU, hrpW, hrpO, hrpX, hrpY, predicted encode proteins 14,795, 23,211, 9,381, 28,489, 39,957 Da,...
Abstract Characteristic symptoms of Pierce's disease ( PD ) in grapevines V itis vinifera L .) were observed 2002 the major grape production fields central Taiwan. Disease severity vineyards varied, and all investigated cultivars affected. Diseased tissues collected from for subsequent isolation characterization causal agent X ylella fastidiosa) . Koch's postulates fulfilled by artificially inoculating two purified bacteria to K yoho, H oney R ed G olden M uscat. The inoculated plants...
A Gram-stain-negative, nutritionally fastidious bacterium (PLS229T) causing pear leaf scorch was identified in Taiwan and previously grouped into Xylella fastidiosa. Yet, significant variations between PLS229T Xylellafastidiosa were noted. In this study, evaluated phenotypically genotypically against representative strains of Xylellafastidiosa, including the currently known subspecies fastidiosa subsp. multiplex 'Xylella fastidiosasubsp.pauca'. Because difficulty vitro culture...
Plant endophytic bacteria live inside host plants, can be isolated from surface-sterilized plant tissues, and are non-pathogenic. These assist plants in obtaining more nutrients improve growth via multiple mechanisms. Certain Gram-negative Burkholderia species, including rhizobacteria, bioremediators, biocontrol strains, have been recognized for their plant-growth-promoting abilities, while other isolates identified as opportunistic or human pathogens. In this study, we observed the auxin...
ABSTRACT The species Pseudomonas syringae encompasses plant pathogens with differing host specificities and corresponding pathovar designations. P. requires the Hrp (type III protein secretion) system, encoded by a 25-kb cluster of hrp hrc genes, in order to elicit hypersensitive response (HR) nonhosts or be pathogenic hosts. DNA sequence analysis hrpC hrpRS operons pv. 61 (brown spot beans), glycinea U1 (bacterial blight soybeans), tomato DC3000 speck tomatos) revealed that 13 genes...
Bacillus mycoides strain BM02 originally isolated from the tomato rhizosphere was found to have beneficial functions in by promoting plant growth and reducing severity of Fusarium wilt caused oxysporum f. sp. lycopersici (Fol). Cytological experiments demonstrated B. reduced Fol invasion spore attachment increasing hyphal deformation hydroponics-grown root tissues. Two volatile antifungal compounds, phenylacetic acid (PAA) methylphenyl acetate (MPA), were identified culture filtrates GC-MS...
Pseudomonas syringae is a plant pathogen whose pathogenicity and host specificity are thought to be determined by Hop/Avr effector proteins injected into cells type III secretion system. P. pv. B728a, which causes brown spot of bean, particularly well-studied strain. The system in encoded hrp (hypersensitive response pathogenicity) hrc (hrp conserved) genes, clustered island with tripartite structure such that the hrp/hrc genes flanked conserved locus an exchangeable (EEL). EELs strain 61,...
Summary The cloned hrp / hrc cluster of Pseudomonas syringae pv. 61 (Pss61) contains 28 proteins, and many those are assembled into a type III secretion system (TTSS) that is responsible for eliciting the hypersensitive response (HR) in non‐host plants causing diseases on host ( Huang et al ., 1995 ). hrpG , second gene hrpC operon, encodes 15.4 kDa cytoplasmic protein whose predicted structure similar to SicP E ‐value: 0.19), TTSS chaperone Salmonella typhimurium . Two non‐polar mutants,...
The draft genome sequence of Xylella fastidiosa pear leaf scorch strain PLS229, isolated from the cultivar Hengshan (Pyrus pyrifolia) in Taiwan, is reported here. bacterium has a size 2,733,013 bp, with G+C content 53.1%. PLS229 was annotated and 3,259 open reading frames 50 RNA genes.
XopDXcc8004, a type III effector of Xanthomonas campestris pv. (Xcc) 8004, is considered shorter version the XopD, which lacks N-terminal domain. To understand functions in planta, transgenic approach combined with inducible promoter to analyze effects XopDXcc8004 Arabidopsis was done. Here, expression elicited accumulation host defense-response genes. These molecular changes were dependent on salicylic acid and correlated lesion-mimic phenotypes observed XVE::XopDXcc8004 plants. Moreover,...
The ability of Pseudomonas syringae to grow in planta is thought be dependent upon the Hrp (type III secretion) system and multiple effector proteins that this injects into plant cells. ORF5 conserved locus P. pv. tomato DC3000 pathogenicity island was shown encode a Hrp-secreted protein have similarly secreted homolog encoded an effector-rich located elsewhere genome. These putative genes were designated hopPtoA1 hopPtoA2, respectively. DNA gel blot analysis revealed sequences hybridizing...
Plant disease induced by Xanthomonas campestris pv. depends on type III effectors but the molecular basis is poorly understood. Here, AvrXccC8004 was characterized, and it found that AvrB_AvrC domain essential sufficient to elicit defense responses in an Arabidopsis-resistant ecotype (Col-0). An upregulation of genes responding shown a profile host gene expression. The changes were correlated with morphological observed phenotypic ultrastructural characterizations. Interestingly, abscisic...
Bacterial galU coding for a uridine diphosphate-glucose pyrophosphorylase plays an important role in carbohydrates biosynthesis, including synthesis of lipopolysaccharides (LPS), membrane-derived oligosaccharides, and capsular polysaccharides. In this study, we characterized the mutant Pseudomonas syringae pv. 61 (Psy61), necrotizing plant pathogen whose pathogenicity depends on functional type III secretion system (T3SS), showed that Psy61 had reduced biofilm formation ability, was...