A5075992765- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Heat shock proteins research
- Plant Disease Resistance and Genetics
- Mycotoxins in Agriculture and Food
- Banana Cultivation and Research
- Enzyme Structure and Function
- Protein Structure and Dynamics
- Plant Pathogens and Resistance
- Plant Stress Responses and Tolerance
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Yeasts and Rust Fungi Studies
- Plant Disease Management Techniques
- Nanoparticles: synthesis and applications
- Molecular Biology Techniques and Applications
- Genetics and Plant Breeding
- Nematode management and characterization studies
- Agriculture, Plant Science, Crop Management
- Endoplasmic Reticulum Stress and Disease
- Nanocomposite Films for Food Packaging
- Plant pathogens and resistance mechanisms
- Plant Parasitism and Resistance
- Heat transfer and supercritical fluids
- Plant Genetic and Mutation Studies
National Chung Hsing University
2008-2023
National Taipei University
2012
Plant Breeding and Acclimatization Institute - National Research Institute
2007
University of Georgia
2007
National Taiwan University
1995-2000
Purdue University West Lafayette
1993-1997
Combinations of ethylene and methyl jasmonate (E/MeJA) synergistically induced members both groups 1 5 the pathogenesis-related (PR) superfamily defense genes. E/MeJA caused a synergistic induction PR-1b osmotin (PR-5) mRNA accumulation in tobacco seedlings. also activated promoter fused to [beta]-glucuronidase marker gene tissue-specific manner. The responsiveness was localized on -248 +45 fragment that exhibited several other inducers. resulted protein levels similar those by osmotic...
A gene encoding the rice 16.9-kDa class I low-molecular-mass (LMM) heat-shock protein (HSP), Oshsp16.9, was introduced into Escherichia coli using pGEX-2T expression vector to analyze possible function of this LMM HSP under heat stress. It is known that E. does not normally produce HSPs. We compared survivability XL1-Blue cells transformed with a recombinant plasmid containing glutathione S -transferase (GST)–Oshsp16.9 fusion (pGST-FL cells) control (pGST (HS) after isopropyl β- d...
Summary A cDNA clone pCZ1, with a 1.1 kb insert, was isolated from NaCI‐adapted tobacco cell library that encodes an apparently full‐length 29 kDa protein (251 amino acids) calculated pl of 5.7. The encoded peptide had high acid sequence identity bovine 14‐3‐3 which originally found as abundant in the animal central nervous system. Recently, proteins to have also been plants, insects and yeast, appear diverse physiological functions. Similar brain protein, recombinant pCZ1 stimulated...
This study developed a novel and inexpensive detection method based on TaqMan probe-based insulated isothermal polymerase chain reaction (iiPCR) for the rapid of Panama disease caused by Fusarium oxysporum f. sp. cubense (Foc) race 4, which is currently among most serious fungal vascular diseases worldwide. By using portable POCKIT™ device with primer set iiFoc-1/iiFoc-2, Foc 4 iiPCR assay (including DNA amplification signal monitoring) could be completed within one hour. The thus...
Successive cultivation of fungi on artificial media has been reported to cause the sectorization, which leads degeneration developmental phenotype, and virulence. Fusarium oxysporum f. sp. niveum (Fon), causal agent watermelon wilt, forms degenerated sectors after successive cultivation. In present research, we demonstrated that subculture with aged mycelia increased incidence degenerations. To further investigate differences between Fon wild type (sporodochial type, ST) variants (MT:...
Fusarium wilt disease of watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai), caused by oxysporum f. sp. niveum (FON), is one the limiting factors worldwide production. In this study, a resistant JSB, which was derived from spontaneous mutation susceptible Sugar Baby (SB), used to investigate histopathology. The number and diameter xylem vessels in root (10 mm below shoot base) JSB plants were significantly higher than those SB plants. At 9 days post inoculation (dpi), using plate...
Journal Article A Recombinant Rice 16.9-kDa Heat Shock Protein Can Provide Thermoprotection in Vitro Get access Ching-Hui Yeh, Yeh Department of Botany, National Taiwan UniversityTaipei, Taiwan, R.O.C. Search for other works by this author on: Oxford Academic Google Scholar Kai-Wun Shu-Hsing Wu, Wu Pi-Fang Linda Chang, Chang Yih-Ming Chen, Chen Chu-Yung Lin 1 1To whom correspondence should be addressed. Plant and Cell Physiology, Volume 36, Issue 7, October 1995, Pages 1341–1348,...
Heat‐stable mycelial extracts of the nonpathogenic fungus Trichoderma longibrachiatum induced resistance in tobacco seedlings ( Nicotiana tabacum L. cv. Wisconsin 38) to pathogen Phytophthora parasitica var. nicotianae (race 0), which did not involve a hypersensitive response. Resistance could be with extract prepared same manner from P. . The expression PR‐1b and osmotin (PR‐5) genes higher level than pathogenic fungus. tissue‐specific pattern PR gene induction by was different that...
SUMMARY Fusarium wilt of watermelon, caused by oxysporum f. sp. niveum , is one the limiting factors for watermelon production in Taiwan. In recent research, phenylalanine ammonia lyase (PAL) gene expressed shoot base resistant line JSB was related to resistance. Phenylalanine key regulatory enzyme phenylpropanoid metabolic pathway. The downstream products phenolic compounds are considered be involved complicated plant defence mechanisms. They could act as signal molecules, antimicrobial...
Cultured tobacco cells ( Nicotiana tabacum L. cv. Wisconsin 38) adapted to NaCl exhibited significant morphological and physiological changes. Adapted remained smaller more isodiametric at maturity than unadapted cells. The vacuole increased in size relative the cytoplasm an extensive network of transvacuolar membrane strands developed. These changes altered surface contact area between substantially. In addition, Hechtian that anchor cortical structure cell wall became extensively branched...
Plant-parasitic nematodes infect a diversity of crops, resulting in severe economic losses agriculture. Microbial volatile organic compounds (VOCs) are potential agents to control plant-parasitic and other pests. In this study, VOCs emitted by dozen bacterial strains were analyzed using solid-phase microextraction followed gas chromatography-mass spectrometry. Fumigant toxicity selected VOCs, including dimethyl disulfide (DMDS), 2-butanone, 2-pentanone, 2-nonanone, 2-undecanone, anisole,...
A monospecific polyclonal antibody was used to study the tissue-type specificity and intracellular localization of class I low-molecular-weight (LMW) heat-shock proteins (HSPs) in soybean (Glycine max) under different regimes. In etiolated seedlings, root meristematic regions contained highest levels LMW HSP. No tissue-type-specific expression HSP detected using tissue-printing method. immunolocalization studies seedlings treated with HS (40[deg]C for 2 h) HSPs were found aggregated granular...
Full-length coding sequences of the beta-tubulin gene (tubA) were PCR-amplified and sequenced from 42 Phaeosphaeria isolates, including 16 P. nodorum 23 avenaria species cereals, two Polish isolates rye (Secale cereale L.), one isolate dallis grass (Paspalum dilatatum Poir). A tubA size 1556bp was identified in wheat- barley-biotype (PN-w PN-b), f. sp. (Paa), homothallic triticea (P.a.t.) (Pat1) P.a.t. (Pat3) State Washington. The length polymorphisms detected (Pat2) foxtail barley (Hordeum...