We aimed to investigate the association of gut microbiota with disease activity, inflammatory parameters, and auto-antibodies profile in rheumatoid arthritis (RA). A total 138 RA patients 21 healthy controls (HC) were enrolled. Fecal samples collected for bacterial DNA extraction 16S ribosome (r)RNA sequencing, followed by analyses composition. Serum levels tumor necrosis factor (TNF)-α, interleukin (IL)-6, IL-17A determined using ELISA. Our results indicated that had lower diversity index,...

Campylobacter jejuni (C. jejuni) is one of the most common causes human bacterial enteritis worldwide primarily due to contaminated poultry products. Previously, we found a significant difference in C. colonization ceca between two genetically distinct broiler lines (Line A (resistant) has less colony than line B (susceptible) on day 7 post inoculation). We hypothesize that different mechanisms these genetic may affect their ability resist chickens. The molecular local host response chickens...

Previously conducted studies using two chicken lines (A and B) show that line A birds have increased resistance to a number of bacterial protozoan challenges heterophils isolated from are functionally more responsive. Furthermore, when stimulated with Toll-like receptor (TLR) agonists, expressed totally different cytokine chemokine mRNA expression pattern than B. large-scale gene profile an Agilent 44K microarray on B also revealed significantly differential in many immune-related genes...

Salmonella enterica serovar Enteritidis (SE) is one of the most common food-borne pathogens that cause human salmonellosis and usually results from consumption contaminated poultry products. The mechanism SE resistance in chickens remains largely unknown. Previously, heterophils isolated broilers with different genetic backgrounds (SE-resistant [line A] -susceptible B]) have been shown to be important defending against infections. To dissect interplay between infection, we utilized...

Abstract Background The development of microarray technology has greatly enhanced our ability to evaluate gene expression. In theory, the expression all genes in a given organism can be monitored simultaneously. Sequencing chicken genome provided crucial information for design comprehensive transcriptome microarray. A long oligonucleotide been manually curated and designed by group manufactured using Agilent inkjet technology. This provides flexible powerful platform with high sensitivity...

When attempting to probe the genetic makeup of diverse bacterial communities that elude cell culturing, researchers face two primary challenges: isolation rare bacteria from microbial samples and removal contaminating cell-free DNA. We report a compact, low-cost, high-performance microfabricated fluorescence-activated sorting (μFACS) technology in combination with tyramide signal amplification fluorescence situ hybridization (TSA-FISH) address these challenges. The TSA-FISH protocol was...

Upon exposure to 0.25 mM cystine dimethyl ester, normal and cystinotic leukocytes accumulate substantially more intracellular than is present endogenously in cells. Leukocytes loaded by ester may have abnormally lucent distended lysosomes, the compartmentalized within granular fraction of After cells are exposed clearance from much faster The ratios labeled cysteine-N-ethylmaleimide also greater normals cystinotics 60 min after termination loading. No overlap ranges half-times or...

Red yeast Sporidiobolus pararoseus KM281507 has been recognized as a potential feed additive. Beyond their nutritional value (carotenoids and lipids), red cells (RYCs) containing high levels of β-glucan can bind mycotoxins. This study investigated the industrial feasibility large-scale production RYCs, along with ability to act mycotoxin binder. Under semi-controlled pH condition in 300 L bioreactor, 28.70-g/L biomass, 8.67-g/L lipids, 96.10-mg/L total carotenoids were obtained, RYCs found...

Campylobacter jejuni is one of the most common causes acute enteritis worldwide. Chickens are believed to be main reservoir C. jejuni. The role that host genetics play in resistance/susceptibility colonization broilers still not clear. Day-old from 2 parental lines (A and B) their F1 reciprocal crosses (C D) were challenged orally with 105 cfu address determining broilers. Cloacal swabs collected on 6, 10, 13 d postinoculation (dpi), cecal contents cultured for 7 14 dpi. number colonies...

Gut microbiota are reported to be associated with many diseases, including cancers. Several bacterial taxa have been shown cancer development or response treatment. However, longitudinal alterations during the of cancers relatively unexplored. To better understand how changes, we profiled gut composition from prostate cancer-bearing mice and control at five different time points. Distinct differences were found between mice.

The NF-kB pathway plays an important role in regulating the immunity response animals. In this study, small interfering RNAs (siRNA) were used to specifically inhibit 1 expression and elucidate of signal transduction Salmonella challenge chicken HD11 cell line. cells transfected with either siRNA, glyceraldehyde 3-phosphate dehydrogenase siRNA (positive control) or negative control for 24 h, followed by enteritidis (SE) non-challenge h 4 h. Eight candidate genes related SE selected examine...

Summary Campylobacter jejuni is one of the most common causes human bacterial enteritis worldwide. The molecular mechanisms host responses chickens to C. colonization are not well understood. We have previously found differences in at 7‐days post‐inoculation (pi) between two genetic broiler lines. However, within each line, all birds were colonized by (27.5% line A, and 70% B). Therefore, objective present experiments was further define gene expression non‐colonized line. RNA isolated from...

Little is known about the degradability of mycotoxin deoxynivalenol (DON) by spent mushroom substrate (SMS)-derived manganese peroxidase (MnP) and lignin (LiP) its potential. The present study investigated growth inhibition Fusarium graminearum KR1 degradation DON MnP LiP extracted from SMS. results 7-day treatment period showed that mycelium F. were 23.7% 74.7%, respectively. Deoxynivalenol production in was undetectable after with 50 U/mL or for 7 days. N-acetyl-D-glucosamine (GlcNAc)...

Mycotoxin removers include enzymes and adsorbents that may be used in animal feeds to eliminate the toxic effects of mycotoxins. This study aimed determine removability two different types mycotoxin removers, enzyme degradation reagents (EDRs), simulated gastrointestinal conditions pigs poultry. Seven commercial including five EDRs adsorbents, were tested vitro. In this study, supplemented dosages pig poultry recommendation ranging from 0.05% 0.2%. For pigs, vitro gastric small intestinal...

Plurihormonal pituitary adenomas: immunostaining of all hormones is mandatory for correct classification Aims : We studied the clinicopathological characteristics plurihormonal adenomas. Methods and results The study material included 167 adenomas, which consisted 31% surgically removed adenomas that we collected during a 12‐year period. mean age patients with adenoma was 45.7 years (range 13–75 years). There were 86 men 81 women. All tumours fully classified by immunohistochemical staining...

Marek's Disease (MD) is a hyperproliferative, lymphomatous, neoplastic disease of chickens caused by the oncogenic Gallid herpesvirus type 2 (GaHV-2; MDV). Like several human lymphomas MD lymphoma cells overexpress CD30 antigen (CD30hi) and are in minority, while non-neoplastic (CD30lo) form majority population. unique natural in-vivo model CD30hi with both lymphomagenesis spontaneous regression. The exact mechanism transformation from CD30lo expressing phenotype to unknown. Here, using...

Avian influenza virus (AIV) is a major respiratory disease of poultry that causes catastrophic losses to the industry. The Mx protein has been shown confer antiviral responses viruses in mice. One nonsynonymous substitution (S631N) chicken reported be associated with resistance AIV infection vitro. previous studies suggested controversy over whether this plays an important role chicken. It would intriguing investigate if both ovo and vivo chickens. In study, embryos young chicks were...

Fumonisin B1 (FB1) is among the most common contaminants produced by Fusarium spp. fungus from corns and animal feeds. Although FB1 has been known to cause physical or functional defects of embryos in humans several species such as Syrian hamsters, rabbits, rodents, little about precise toxicity underlying mechanisms have not fully addressed. The present study aimed investigate its developmental potential action on sphingolipid metabolism Brown Tsaiya Ducks (BTDs) embryos. We examined effect...

ABSTRACT Prochlorococcus and Synechococcus are the most abundant photosynthetic organisms in oligotrophic waters responsible for a significant percentage of earth's primary production. Here we developed method metagenomic sequencing sorted populations using transposon-based library preparation technique. First, observed that cell lysis technique associated amount input DNA had an important role determining quality. Second, found our provided more even coverage distribution matched sequences...