It has previously been shown that highly invasive MDA-MB231 human breast cancer cells express vacuolar proton-translocating ATPase (V-ATPases) at the cell surface, whereas poorly MCF7 line does not. Bafilomycin, a specific V-ATPase inhibitor, reduces in vitro invasion of MB231 but not cells. Targeting V-ATPases to different cellular membranes is controlled by isoforms subunit a. mRNA levels for were measured and using quantitative reverse transcription-PCR. The results show although all four...

Abstract Phenotypes of the tomato (Solanum lycopersicum) high pigment-2dg (hp-2dg) and hp-2j mutants are caused by lesions in gene encoding DEETIOLATED1, a negative regulator light signaling. Homozygous hp-2dg plants display plethora distinctive developmental metabolic phenotypes comparison to their normal isogenic counterparts. These are, however, best known for increased levels carotenoids, primarily lycopene, other plastid-accumulating functional metabolites. In this study we analyzed...

Tomato yellow leaf curl virus (TYLCV) is a devastating disease of tomato (Solanum lycopersicum) that can be effectively controlled by the deployment resistant cultivars. The TYLCV-resistant line TY172 carries major recessive locus for TYLCV resistance, designated ty-5, on chromosome 4. In this study, association between 27 polymorphic DNA markers, spanning ty-5 locus, and resistance characteristics individual plants inoculated with in 51 segregating recombinant populations were analyzed....

Temperature is one of the main external factors affecting anthocyanin accumulation in plant tissues: low temperatures cause an increase and elevated a decrease concentration. Several metals have been shown to half-life time anthocyanins, by forming complexes with them. We studied combined effect increased metal concentrations on anthocyanins aster 'Sungal' flowers. It has found that magnesium treatment plants or detached flower buds, partially prevents colour fading at temperatures....

Tonoplast enriched membrane vesicle fractions were isolated from unadapted and NaCl (428 millimolar) adapted tobacco cells (Nicotiana tabacum L. var Wisconsin 38). Polypeptides the tonoplast separated by SDS-PAGE analyzed Western blots using polyclonal antibodies to 70 kilodalton subunit of red beet H(+)-ATPase. These cross-reacted exclusively a polypeptide an apparent molecular weight 69 kilodaltons. The inhibited ATP-dependent, NO(3) (-) sensitive H(+) transport into vesicles in both...

The tomato Anthocyanin fruit (Aft) genotype is characterized by purple color in skin and outer pericarp of its fruits due to higher levels anthocyanins—flavonoid metabolites. Our objectives were carry out metabolic molecular characterization this genotype, emphasizing interaction with the high pigment-1 (hp-1) mutation, known increase flavonoids fruits. These fit growing interest developing functional results show that 1) Aft are also significantly flavonols quercetin kaempferol, thus...

Microvascular endothelial cells involved in angiogenesis are exposed to an acidic environment that is not conducive for growth and survival. These must exhibit a dynamic intracellular (cytosolic) pH (pHcyt) regulatory mechanism cope with acidosis, addition the ubiquitous Na+/H+ exchanger HCO3--based H+-transporting systems. We hypothesize presence of plasmalemmal vacuolar-type proton ATPases (pmV-ATPases) allows microvascular better this pmV-ATPases required cell migration. This study...

Fruits of tomato plants carrying the high pigment-1 mutations hp-1 and hp-1w are characterized by an increased number plastids coupled with enhanced levels functional metabolites. Unfortunately, mutant also typified light-dependent retardation in seedling whole-plant growth development, which limits their cultivation. These were mapped to gene encoding UV-DAMAGED DNA BINDING PROTEIN 1 (DDB1) and, recently, fruit-specific RNA interference studies have demonstrated carotenoid accumulation...

Abstract T2 ribonucleases (RNases) are RNA‐degrading enzymes that function in various cellular processes, mostly via RNA metabolism. RNase‐encoding genes have been identified organisms, from bacteria to mammals, and most diverse plants. The existence of RNase almost every organism suggests an important biological has conserved through evolution. In plants, RNases suggested be involved phosphate scavenging recycling, implicated defence responses pathogens. We investigated the tomato LE, known...

Abstract In this study we examined the ability of interferon‐γ (IFN‐γ) to regulate mammary epithelial cell growth and gene expression, with particular emphasis on two genes: Maspin (a member serine protease inhibitor superfamily), lysosomal aspartyl endopeptidase cathepsin D ( CatD ). The protein products these genes are critically involved in regulation multitude biological functions different stages tissue development remodeling. addition, expression is down‐regulated primary breast cancer...

Plant mitochondrial genomes (mtDNAs) are large and undergo frequent recombination events. A common phenotype that emerges as a consequence of altered mtDNA structure is cytoplasmic-male sterility (CMS). The molecular basis for CMS remains unclear, but it seems logical respiration activities would result in reduced pollen production. Analysis tobacco (Nicotiana tabacum) mtDNAs indicated CMS-associated loci often contain fragments known organellar genes. These may assemble with complexes...

Shoot regeneration is a key tool of modern plant biotechnology. While many researchers use this process empirically, very little known about the early molecular genetic factors and signaling events that lead to shoot regeneration. Using tobacco as model system, we found inductive required for occur in first 4–5 days following incubation on medium. Leaf segments placed medium did not produce shoots if removed from before four indicating time frame crucial induction longer than five maintain...

Tomato (Solanum lycopersicum) fruits expressing a yeast S-adenosyl methionine decarboxylase (ySAMdc) gene under control of ripening-induced promoter show altered phytonutrient content and broad changes in expression. Genome-wide transcriptional alterations pericarp tissues the ySAMdc-expressing are shown. Consistent with ySAMdc expression pattern from promoter, very minor were detected at mature green developmental stage. At breaker red stages, levels numerous transcripts observed general...

ABSTRACT In immunobiochemical blots, polyclonal antibodies against subunits of plant and mammalian vacuolar-type ATPases (V-ATPases) cross-react strongly with corresponding larval Manduca sexta midgut plasma membrane V-ATPase. Thus, rabbit antiserum Kalanchoe daigremontiana tonoplast V-ATPase holoenzyme cross-reacts the 67, 56, 40, 28 20kDa separated by SDS-PAGE. Antisera bovine chromaffin granule 72 39 kDa 67 43 57 subunit both beet root oat 56 subunit. immunocytochemical light micrographs,...

Fruits harvested from tomato (Solanum lycopersicum) plants carrying mutations at the DAMAGED DNA BINDING PROTEIN 1 gene (SlDDB1; hp-1 and hp-1(w)) are characterized by significantly elevated levels of lycopene several other phytonutrients. We hypothesize that pleotropic effect on mutant Slddb1 some general function DDB1 protein cell growth. The main objective this research was to carry out functional analysis SlDDB1 alleles both in-planta ex-planta in comparison their normal counterparts....

The tomato INTENSE PIGMENT (IP) genotype is characterized by intense visual pigmentation of unripe and ripe fruits, not thoroughly analyzed thus far. This study was therefore designed to analyze key morphologic, metabolomic, photomorphogenic phenotypes this in comparison its near-isogenic normal counterpart evaluate significance relative other mutants known for increased fruit pigmentation. IP produced smaller darker red a substantially chloroplast biogenesis found green leaf tissues....

Cultured tobacco cells ( Nicotiana tabacum L. cv. Wisconsin 38) adapted to NaCl exhibited significant morphological and physiological changes. Adapted remained smaller more isodiametric at maturity than unadapted cells. The vacuole increased in size relative the cytoplasm an extensive network of transvacuolar membrane strands developed. These changes altered surface contact area between substantially. In addition, Hechtian that anchor cortical structure cell wall became extensively branched...