A5043405777- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Studies on Chitinases and Chitosanases
- Enzyme Production and Characterization
- Nematode management and characterization studies
- Plant Pathogenic Bacteria Studies
- Fungal Plant Pathogen Control
- Plant Disease Resistance and Genetics
- Plant tissue culture and regeneration
- Transgenic Plants and Applications
- Renal Diseases and Glomerulopathies
- Plant Disease Management Techniques
- Probiotics and Fermented Foods
- Tropical and Extratropical Cyclones Research
- Photosynthetic Processes and Mechanisms
- Plant Parasitism and Resistance
- Nanocomposite Films for Food Packaging
- Cardiovascular and exercise physiology
- Chronic Kidney Disease and Diabetes
- Ubiquitin and proteasome pathways
- CRISPR and Genetic Engineering
- Musculoskeletal pain and rehabilitation
- Protein Hydrolysis and Bioactive Peptides
- Polysaccharides and Plant Cell Walls
National Taiwan University
2012-2025
Taichung Veterans General Hospital
2024
Kaohsiung Medical University
2023
Kaohsiung Medical University Chung-Ho Memorial Hospital
2023
Beam Therapeutics (United States)
2023
Capital Institute of Pediatrics
2021-2022
Feng Chia University
2017
University of California, Davis
1994-2015
Tungnan University
2013
CDC Foundation
2011
Bacillus cereus 28-9 is a chitinolytic bacterium isolated from lily plant in Taiwan. This exhibited biocontrol potential on Botrytis leaf blight of as demonstrated by detached assay and dual culture assay. At least two chitinases (ChiCW ChiCH) were excreted B. 28-9. The ChiCW-encoding gene was cloned moderately expressed Escherichia coli DH5<TEX>$\alpha$</TEX>. Near homogenous ChiCW obtained the periplasmic fraction E. cells harboring chiCW purification procedure. An vitro showed that...
Transient gene expression via Agrobacterium-mediated DNA transfer offers a simple and fast method to analyze transgene functions. Although Arabidopsis is the most-studied model plant with powerful genetic genomic resources, achieving highly efficient consistent transient for function analysis in remains challenging.We developed robust system, named AGROBEST (Agrobacterium-mediated enhanced seedling transformation), which achieves versatile of diverse functions intact seedlings. Using...
Abstract BACKGROUND: Bacillus cereus C1L is a plant growth‐promoting rhizobacterium and can elicit induced systemic resistance (ISR) in plants against necrotrophic pathogens. However, little known about ISR elicitors produced by B. C1L, no elicitor has been identified characterised. Therefore, the objective of this study to identify volatile elicitor(s) C1L. RESULTS: The metabolites were extracted, separated solid‐phase microextraction, gas chromatography mass spectrometry. Dimethyl...
The influence of daily spontaneous running (DSR) and gender on the arterial baroreflex regulation heart rate (HR) lumbar sympathetic nerve activity (LSNA) was examined in 13 male [7 sedentary (SED) 6 DSR] 12 female (6 SED DSR) Sprague-Dawley rats. After 8-9 wk DSR or control, all animals were chronically instrumented with right femoral venous left carotid catheters electrodes around trunk. resulted an increase weight-to-body weight ratio (P = 0.001) rats resting bradycardia 0.001). Arterial...
ABSTRACT Black rot caused by hemibiotrophic Xanthomonas campestris pv. ( Xcc ) is a great problem in crucifer crop production. Various host responses are activated upon attack; however, their roles black resistance remain ambiguous. In this study, highly of plants was achieved applying field‐screened systemic resistance‐eliciting Bacillus velezensis strain 37‐1. The contributions 37‐1‐altered to were then investigated Arabidopsis . Hypersensitive response and hydrogen peroxide accumulation...
Chronic hepatitis B virus (HBV) infection remains a global health problem due to the lack of treatments that prevent viral rebound from HBV covalently closed circular (ccc)DNA. In addition, DNA integrates in human genome, serving as source surface antigen (HBsAg) expression, which impairs anti-HBV immune responses. Cytosine base editors (CBEs) enable precise conversion cytosine into thymine within DNA. this study, CBEs were used introduce stop codons genes,
To assess the effectiveness of 3 different health promotion exercise programs for work-related shoulder and neck pain.Comparative study design.A total 178 employees were recruited grouped.Those in "Self-exercise group" (n = 56) given a lecture about program then performed by themselves during their office break. "Team-exercise group I" 69) once under supervision physiotherapist after lecture. II" 14) twice; physiotherapist's supervision. A modified Nordic questionnaire, pain threshold...
SUMMARY Ferredoxin-I (Fd-I) is a fundamental protein that involved in several metabolic pathways. The amount of Fd-I found plants generally regulated by environmental stress, including biotic and abiotic events. In this study, the correlation between quantity plant disease resistance was investigated. levels were increased inoculation with Pseudomonas syringae pv. but reduced Erwinia carotovora ssp. carotovora. Transgenic tobacco over-expressing sense sweet pepper gene (pflp) resistant to E....
Southern corn leaf blight (SCLB) is an important foliar disease of maize. In this study, induced systemic resistance (ISR)‐eliciting rhizobacterium Bacillus cereus C1L was used to protect maize against SCLB. Application B. in rhizosphere effectively protected from SCLB under greenhouse and field conditions. The protection effect similar that Maneb (2 kg active ingredient per hectare), a recommended fungicide. Furthermore, possible factors elicit ISR promote plant growth were investigated....
Lily leaf blight, caused by Botrytis elliptica, is an important fungal disease in Taiwan. In order to identify effective, nonfungicide method decrease incidence Lilium formosanum, the efficacy of rhizobacteria eliciting induced systemic resistance (ISR) was examined this study. Over 300 were isolated from rhizosphere L. formosanum healthy plants and 63 identified analysis fatty acid profiles. Disease suppressive ability 13 strains demonstrated soil drench application bacterial suspensions...
Bacillus subtilis F293 is an antagonistic bacterium against a wide range of fungal species. In order to determine the effect chitinase on antifungal activity B. F293, 2.4-kb DNA fragment containing chiA gene circulans WL-12 was ligated into shuttle vector pHY300PLK and transformed F293. A bioassay conducted culture supernatant showed that, in comparison control strain, expressing exhibited greater inhibition spore germination Botrytis elliptica, indicating that could enhance function...
Agrobacterium tumefaciens is a phytopathogenic bacterium that causes crown gall disease by transferring transferred DNA (T-DNA) into the plant genome. The translocation process mediated type IV secretion system (T4SS) consisting of VirD4 coupling protein and 11 VirB proteins (VirB1 to VirB11). All are required for production T-pilus, which consists processed VirB2 (T-pilin) VirB5 as major minor subunits, respectively. an essential component T4SS, but roles assembled T-pilus in virulence...
Paenibacillus polymyxa is a beneficial bacterium for plant health. P. TP3 exhibits antagonistic activity toward Botrytis cinerea and alleviates gray mold symptoms on the leaves of strawberry plants. Moreover, suppression flowers fruits plants in field trials, including vegetative cells endospores, was demonstrated, indicating potential strain as biological control agent. To examine anti-B. compounds produced by TP3, we performed matrix-assisted laser desorption/ionization time-of-flight mass...
Exercise training attenuates arterial baroreflex function. Mechanisms responsible may include an attenuated aortic baroreceptor reactivity (afferent mechanisms) and/or central gain. We tested the hypothesis that gain is by daily spontaneous running (DSR). Eighteen anesthetized Sprague-Dawley rats (11 control and 7 DSR) were tracheotomized instrumented with femoral venous right carotid catheters. Electrodes placed around left depressor nerve lumbar sympathetic trunk. Eight to thirteen weeks...
Bacillus cereus strain C1L, screened from the rhizosphere of Lilium formosanum, prevents severe lily leaf blight. The purpose this study was to formulate a new biopesticide by microencapsulating C1L enhance residual stability. In addition, since viable cell number is an important factor affecting biological effects fungicide, also used response surface methodology and sequential quadratic programming methods optimize best formula for maximum viability microencapsulated spray drying....
The IncW plasmid pSa originally derived from Shigella flexneri completely inhibits the tumor-inducing ability of Agrobacterium tumefaciens when it is resident in this organism. Oncogenic inhibition mediated through expression osa gene on pSa. This part a 3.1-kb DNA segment that contains four open reading frames revealed by sequencing. Specific deletions and TnCAT insertions within localized oncogenic inhibitory activity to last frame, orf-4, designated (for suppression activity). No promoter...
Bacillus cereus C1L was demonstrated to induce systemic resistance in Lilium formosanum against leaf blight caused by Botrytis elliptica. Suppression subtractive hybridization library of L. triggered B. were screened and 3 differentially expressed genes identified. Based on sequence analysis, these encoding putative glycine-rich protein, metallothionein-like PsbR protein photosystem 2, designated LfGRP1, LfMT1, LfPsbR, respectively. The results Northern blot analysis showed that expressions...
Breast cancer treatments often have negative effects on fertility, which pose challenges among patients who want to be parents in the future. This study aimed examine efficacy of oocyte cryopreservation, embryo and ovarian tissue cryopreservation with breast cancer. retrospective review evaluated 42 underwent fertility preservation at our center from January 2012 December 2022. encompassed demographic characteristics patients, stages, treatment details, types procedures their outcomes. The...