A5003582372- Chromosomal and Genetic Variations
- Plant and animal studies
- Plant Reproductive Biology
- Plant Disease Resistance and Genetics
- Plant Molecular Biology Research
- Plant tissue culture and regeneration
- Flowering Plant Growth and Cultivation
- Genetic Mapping and Diversity in Plants and Animals
- Plant Parasitism and Resistance
- Plant Genetic and Mutation Studies
- Plant Virus Research Studies
- Plant and Fungal Species Descriptions
- Phytase and its Applications
- Seed Germination and Physiology
- Photosynthetic Processes and Mechanisms
- Chemical synthesis and alkaloids
- Cassava research and cyanide
- Plant nutrient uptake and metabolism
- Soybean genetics and cultivation
- GABA and Rice Research
- Plant Pathogens and Fungal Diseases
- Legume Nitrogen Fixing Symbiosis
- Plant Diversity and Evolution
- Proteins in Food Systems
- Microbial Metabolism and Applications
Institute of Plant and Microbial Biology, Academia Sinica
2011-2021
Academia Sinica
1991-2014
National Pingtung University of Science and Technology
2009
National Taiwan University
2007-2009
University of Calgary
2007
Taiwan Agricultural Research Institute Council of Agriculture, Executive Yuan
2005
Czech Academy of Sciences, Institute of Botany
1997
Summary The Phalaenopsis orchid produces complex flowers that are commercially valuable, which has promoted the study of its flower development. E‐class MADS ‐box genes, SEPALLATA ( SEP ), combined with B‐, C‐ and D‐class involved in various aspects plant development, such as floral meristem determination, organ identity, fruit maturation, seed formation architecture. Four ‐like genes were cloned from orchid, duplicated Pe s grouped into 1/3 2/4 . All expressed all organs. 2 expression was...
• Background and Aims Owing to large-scale collecting, the lady's slipper orchid, Paphiopedilum delenatii, is under threat of extinction. Asymbiotic germination provides a useful way re-establish plants in wild for commercial propagation. A detailed study embryo development would provide information on subsequent events aid propagation species. Methods Developing capsules were collected histochemical ultrastructural studies by using both light transmission electron microscopy. Key Results...
The genes encoding for 18S-5.8S-28S ribosomal RNA (rDNA) are both conserved and diversified. We used rDNA as probe in the fluorescent situ hybridization (rDNA-FISH) to localized rDNAs on chromosomes of 15 accessions representing ten Oryza species. These included cultivated wild species rice, four them tetraploids. Our results reveal polymorphism number loci, repeats, their chromosomal positions among numbers loci varies from one eight locus located at end short arm chromosome 9 is genus...
Lady's slipper orchids (Paphiopedilum) are of high value in floriculture, and interspecific hybridization has long been used for breeding improved cultivars; however, information regarding the genome affinities species chromosome pairing behaviour hybrids remains almost unknown. The present work analyses meiotic by genomic situ cytologically evaluates relationships among parental species.Eight F(1) Paphiopedilum various subgenera or sections were investigated this study. meiosis these was...
This investigation documents the key anatomical features in embryo development of Cypripedium formosanum Hayata, association with ability embryos to germinate vitro, and examines effects culture media seed pretreatments on germination. A better understanding zygotic embryogenesis for L. species would provide insights into subsequent germination events aid vitro propagation these endangered species. In seeds collected at 60 days after pollination (DAP), soon fertilization, no was recorded....
Background and Aims Although abscisic acid (ABA) is commonly recognized as a primary cause of seed dormancy, there lack information on the role ABA during orchid development. In order to address this issue, localization quantification were determined in developing seeds Cypripedium formosanum. Methods The endogenous profile was measured by enzyme-linked immunosorbent assay (ELISA). Temporal spatial distributions visualized immunohistochemical staining with monoclonal antibodies. Fluoridone...
Phalaenopsis is one of the most popular potted plants in horticultural market because colorful and long-lasting flowers. In Phalaenopsis, interspecific hybridization between sections or subgenera has long been used for breeding cultivars with novel traits. Considering diverse genome size karyomorphology species breeding, a study chromosome composition would be crucial to provide fundamental knowledge breeding. this study, we characterized 60 representative various colors types commonly...
Changes in endogenous abscisic acid (ABA) concentrations were investigated developing seeds and the pretreated of Calanthe tricarinata , a hard-to-germinate terrestrial orchid. ABA concentration was as low 2.16 to 2.26 ng·mg −1 fresh weight at proembryo stage [60 90 days after pollination (DAP)] then continuously increased 11.6 210 DAP. Seed maturation accompanied by dramatic decrease water content prominent accumulation protein lipid bodies within embryo proper. The optimum time for...
Abstract A wounded gene WI12 was used as a marker to examine the interaction between biotic stress (wounding) and abiotic (high salt) in facultative halophyte ice plant (Mesembryanthemum crystallinum). The deduced amino acid sequence has 68% similarity WUN1, known potato (Solanum tuberosum) wound-induced protein. Wounding, methyl jasmonate, pathogen infection induced localWI12 expression. Upon wounding, expression ofWI12 reached maximum level after 3 h 4-week-old juvenile leaves, whereas 24...
The phytohormone abscisic acid (ABA) is involved in regulating seed dormancy and germination. A crucial step of ABA biosynthesis higher plants the oxidative cleavage cis-epoxycarotenoids by 9-cis-epoxycarotenoid dioxygenase (NCED). Seed development orchids unusual because embryos are minute size, without obvious histodifferentiation, lack endosperm. To understand regulation orchid seeds, we isolated characterized a full-length cDNA encoding an NCED homologue, PtNCED1, from developing seeds...
Phalaenopsis is a horticulturally important genus of c. 63 spp. native to Southeast Asia. Aiming understand the genome differentiation and phylogenetic relationships in diploid spp., we investigated nuclear DNA content by flow cytometry karyomorphology mapped rDNA loci fluorescence situ hybridization address differentiation. Most species have chromosome complement 2n = 38 (but different karyotype compositions). Chromosome reduction only observed section Aphyllae (2n 34 36). The sizes differ...
1-Aminocyclopropane-1-carboxylic acid (ACC) oxidase catalyzes the oxidation of ACC to gaseous plant hormone, ethylene. Although enzyme does not contain a typical N-terminal consensus sequence for transportation across endoplasmic reticulum (ER), it has recently been shown locate extracellularly by immunolocalization study. It was interest examine whether contains signal peptide that is overlooked structure prediction. We observed in vitro translated apple co-processed or imported canine...
In many eukaryotic organisms, homeobox genes are important regulators that specify the cell fate and body plan in early embryogenesis. this study, a gene designated OSTF1 (Oryza sativa transcription factor 1) encoding homeodomain protein rice was isolated characterized. The encoded OSTF1, although sharing only approximately 51% sequence identity with other HD-GL2 members, contains four characteristic motifs (an N-terminal acidic region, homeodomain, truncated leucine zipper, START domain)....
Adlay (Coix lachryma-jobi L. var. ma-yuen Stapf) is regarded as a nutritive food source well herbal medicine. The nutrition consequence of its high protein content and superior amino acid composition. From ca. 200 expressed sequence tag (EST) sequences in maturing adlay grains, clones encoding precursor polypeptides 10 seed storage proteins the prolamin family, including 8 α-coixin isoforms, 1 δ-coixin, γ-coixin, were identified. Full-length cDNA fragments these coixins obtained by PCR...
Lycoris species of the Amaryllidaceae are important ornamental and medicinal plants in Asia. Karyotypes have been studied extensively since time when their chromosome numbers were first counted over 80 years ago. Based on karyotype, taxa can be classified into monomorphic A group, dimorphic MT sterile dikaryotype MT-A group. Numerous reports dealing with karyotype analysis phylogenetic relationship genus published. However, there disputes controversies regarding evolution resulting from...