A5103115352- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Virus Research Studies
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Plant and Fungal Interactions Research
- Pediatric Hepatobiliary Diseases and Treatments
- Plant Disease Resistance and Genetics
- Infant Nutrition and Health
- CRISPR and Genetic Engineering
- Neonatal Respiratory Health Research
- Genomics and Phylogenetic Studies
- Autophagy in Disease and Therapy
- Polar Research and Ecology
- Reproductive Biology and Fertility
- Immune Cell Function and Interaction
- Plant Pathogenic Bacteria Studies
- Plant and animal studies
- Climate change and permafrost
- Cancer-related molecular mechanisms research
- Bryophyte Studies and Records
- Sperm and Testicular Function
- Plant Taxonomy and Phylogenetics
- Horticultural and Viticultural Research
- Pancreatic function and diabetes
National Taiwan University
2018-2025
Xuzhou Medical College
2020-2024
Second People’s Hospital of Huai’an
2020
Huaian First People’s Hospital
2020
Nanjing Medical University
2020
Guangzhou Medical University
2018
Third Affiliated Hospital of Guangzhou Medical University
2018
National Cheng Kung University
2014
Summary The Phalaenopsis orchid produces complex flowers that are commercially valuable, which has promoted the study of its flower development. E‐class MADS ‐box genes, SEPALLATA ( SEP ), combined with B‐, C‐ and D‐class involved in various aspects plant development, such as floral meristem determination, organ identity, fruit maturation, seed formation architecture. Four ‐like genes were cloned from orchid, duplicated Pe s grouped into 1/3 2/4 . All expressed all organs. 2 expression was...
Helper-component proteinase (HC-Pro), encoded by potyviruses, function as viral suppressors of RNA silencing (VSRs). Despite their conserved role, HC-Pros share approximately 40% similarity, implying potential differences in VSR efficiency, particularly ability to inhibit HEN1 methyltransferase activity. This study investigated the inhibitory from different potyviruses transgenic plants. P1/HC-Pro turnip mosaic virus (P1/HC-ProTu) exhibited most potent inhibition HEN1, followed zucchini...
Abstract Background To investigate the mechanism of RNA silencing suppression, genetic transformation viral suppressors (VSRs) in Arabidopsis integrates ectopic VSR expression at steady state, which overcomes variations caused by different virus infections or limitations host range. Moreover, identifying insertion transgenic gene is necessary to establish a model plant for functional study VSR. Methods Developing an endogenous AGO1-based vitro RNA-inducing complex (RISC) assay prompts...
Abstract The miR390-derived TAS3 trans-acting short-interfering RNAs (tasiRNAs) module represents a conserved RNA silencing pathway in the plant kingdom; however, its characterization bryophyte Marchantia polymorpha is limited. This study elucidated that MpDCL4 processes MpTAS3 double-stranded (dsRNA) to generate tasiRNAs, primarily from 5ʹ- and 3ʹ-ends of dsRNA. Notably, we discovered novel tasiRNA, tasi78A, which can negatively regulate cytochrome P450 gene, MpCYP78A101. Additionally,...
With the growing demand for its ornamental uses, African violet (Saintpaulia ionantha) has been popular owing to variations in color, shape and rapid responses artificial selection. Wild type (WT) is characterized by flowers with bilateral symmetry yet reversals showing radially symmetrical such as dorsalized actinomorphic (DA) ventralized (VA) peloria are common. Genetic crosses among WT, DA, VA revealed that these floral transitions likely be controlled three alleles at a single locus...
Abstract Genomes of potyviruses, the largest group plant viruses, encode HC-Pro proteins that mediate RNA silencing suppression. HC-Pros may exhibit only 40% similarity between species, and induce different levels in autophagic ARGONAUTE1 (AGO1) degradation. Our data indicated turnip mosaic virus (HC-Pro Tu ) could efficiently trigger AGO1 degradation through autophagy compared with zucchini yellow Zy tobacco etch Te ). Furthermore, , but not forms a suppression body (S-body) to recruit...
The P1/HC-Pro viral suppressor of potyvirus suppresses posttranscriptional gene silencing (PTGS). fusion protein can be cleaved into P1 and HC-Pro through the self-cleavage activity, is necessary sufficient to enhance PTGS suppression HC-Pro. To address modulation regulatory relationships induced by turnip mosaic virus (TuMV) (P1/HC-ProTu), a comparative transcriptome analysis three types transgenic plants (P1Tu, HC-ProTu, P1/HC-ProTu) were conducted using both high-throughput (HTP)...
Abstract Premise Transient gene expression systems are powerful tools for studying interactions in plant species without available or stable genetic transformation protocols. We optimized a petal protoplast protocol Sinningia speciosa , model plant, to study the development of floral symmetry. Methods and Results A high yield protoplasts was obtained using 6‐h enzyme digestion solution 1.5% cellulase 0.4% macerozyme. Modest transfection efficiency (average 41.4%) achieved. The viability...
The establishment of dorsal-ventral (DV) petal asymmetry is accompanied by differential growth DV size, shape, and color differences, which enhance ornamental values. Genes involved in flower symmetry Sinningia speciosa have been identified as CYCLOIDEA (SsCYC), but gene regulatory network (GRN) associated with SsCYC to establish still unknown. To uncover the GRN asymmetry, we 630 differentially expressed genes (DV-DEGs) from RNA-Seq dorsal ventral petals wild progenitor, S. 'ES'. Validated...
As a model plant for bryophytes, Marchantia polymorpha offers insights into the role of RNA silencing in aiding early land plants navigate challenges posed by high-temperature environments. Genomic analysis revealed unique ARGONAUTE1 ortholog gene (MpAGO1) M. polymorpha, which is regulated two species-specific microRNAs (miRNAs), miR11707.1 and miR11707.2. Comparative studies small profiles from cellular MpAGO1 immunoprecipitation (MpAGO1-IP) at various temperatures, along with analyses...
This is a protocol to produce stable silencing efficacy and efficiency for VIGS using CymMV as vector floral functional genomics in Phalaenopsis orchids. established based on method created by Lu et al. (2007), then modified Hsieh (2013a; 2013b), (2012) successfully engineered cloning (pCymMV-Gateway) that the target gene fragment simple insert can be manipulated with high efficiency. The inoculated into plants Agro-inoculation Agrobacterium tumefaciens (A. tumefaciens) strain EHA105....
Objective To explore the pathogenesis of non-obstructive azoospermia at molecular level, aiming to provide novel ideas for clinical diagnosis and treatment. Methods The R language was utilized normalize gene chip data downloaded from Gene Expression Omnibus (GEO) screen differentially expressed genes. DAVID KEGG databases were employed carry out ontology function genes enrichment analysis signaling pathways. A differentially-expressed co-expression network delineated by using...
Long non-coding RNAs (lncRNAs) were involved in the development and regulation of necrotizing enterocolitis (NEC) premature infants. To investigate changes lncRNA expression profile intestinal tissues NEC for its possible mechanisms.Intestinal samples collected from 11 patients with who needed surgery(the group), 7 neonatal non-NEC surgery (the Control group).LncRNA's (3 group 3 group) analyzed high-throughput sequencing.Part remaining detected by real-time polymerase chain reaction...
Abstract Ectopically expressing viral suppressors of RNA silencing (VSR) in transgenic Arabidopsis overcome host range limitation and titer variation, have similar VSR levels to investigate suppression. Therefore, identifying the insertion gene developing an endogenous AGO1-based vitro RNA-inducing complex (RISC) assay prompted further investigation into VSR-mediated Three P1/HC-Pro plants from turnip mosaic virus (TuMV) ( Tu ), zucchini yellow Zy tobacco etch Te ) were investigated for RISC...