A5023929903- Invertebrate Immune Response Mechanisms
- Fibroblast Growth Factor Research
- RNA and protein synthesis mechanisms
- Insect symbiosis and bacterial influences
- Bacteriophages and microbial interactions
- Bacterial Genetics and Biotechnology
- Protein Structure and Dynamics
- Epigenetics and DNA Methylation
- Plant Virus Research Studies
- Studies on Chitinases and Chitosanases
- Insect Resistance and Genetics
- Mosquito-borne diseases and control
- Aquaculture disease management and microbiota
- Vibrio bacteria research studies
- Histone Deacetylase Inhibitors Research
- CRISPR and Genetic Engineering
- Poxvirus research and outbreaks
- Bladder and Urothelial Cancer Treatments
- Plant Pathogenic Bacteria Studies
- Machine Learning in Bioinformatics
- DNA Repair Mechanisms
- Plant-Microbe Interactions and Immunity
- Enzyme Structure and Function
- Phytoplasmas and Hemiptera pathogens
- Genomics, phytochemicals, and oxidative stress
Taipei Medical University
2016-2025
Academia Sinica
2011-2024
National Cheng Kung University
2019-2024
National Taiwan University
2006-2023
National Yang Ming Chiao Tung University
2014
Institute of Biological Chemistry, Academia Sinica
2006-2014
The Ohio State University
2001
Significance Since 2009, an emergent shrimp disease, acute hepatopancreatic necrosis disease (AHPND), has been causing global losses to the farming industry. The causative agent of AHPND is a specific strain Vibrio parahaemolyticus . We present evidence here that opportunistic V. becomes highly virulent by acquiring unique AHPND-associated plasmid. This virulence plasmid, which encodes binary toxin [ Photorhabdus insect-related toxins (PirA vp and PirB )] induces cell death, stably inherited...
White spot syndrome virus (WSSV) is a large (≈300 kbp), double-stranded DNA eukaryotic that has caused serious disease in crustaceans worldwide. ICP11 the most highly expressed WSSV nonstructural gene/protein, which strongly suggests its importance infection; but until now, function remained obscure. We show here acts as mimic. In crystal, formed polymer of dimers with 2 rows negatively charged spots approximated duplex arrangement phosphate groups DNA. Functionally, prevented from binding...
High temperature (32 to 33°C) has been shown reduce mortality in white spot syndrome virus (WSSV)-infected shrimps, but the mechanism still remains unclear. Here we show that WSSV-infected shrimps cultured at 32°C, transcriptional levels of representative immediate-early, early, and late genes were initially higher than those 25°C. However, neither IE1 nor VP28 protein was detected suggesting high might inhibit WSSV synthesis. Two-dimensional gel electrophoresis analysis revealed two...
Abstract Background Viral and bacterial diseases can cause mass mortalities in commercial shrimp aquaculture. In contrast to studies on the antiviral response, responses of shrimps infections by high throughput techniques have been reported only at transcriptional level not translational level. this study, a proteomic analysis hemocytes identify differentially expressed proteins response luminous bacterium Vibrio harveyi was evaluated for its feasibility is first time. Results The...
Background and purpose Migraine irritable bowel syndrome (IBS) share many similarities characterized by their epidemiology, periodic pain, lack of definable organic causes, trigger factors, comorbidities proposed pathophysiology. In this retrospective case–control study, the association between migraine IBS was investigated using a nationwide population‐based database in Taiwan. Methods The data were retrieved from National Health Insurance Research Database (NHIRD) all, 14 117 newly...
Purpose: MPT0L145 has been developed as a FGFR inhibitor exhibiting significant anti-bladder cancer activity in vitro and vivo via promoting autophagy-dependent cell death. Here, we aim to elucidate the underlying mechanisms.Experimental Design: Autophagy flux, morphology, intracellular organelles were evaluated by Western blotting, transmission electron microscope, fluorescence microscope. Molecular docking surface plasmon resonance assay performed identify drug-protein interaction....
Abstract T‐cell‐based immunotherapy holds promise for eliminating cancer through T‐cell activation. However, prolonged interaction between T cells and tumors the presence of immunosuppressive factors can diminish cytotoxicity, leading to treatment failure. Here, ribonuclease 1 (RNase1), which degrades RNA, reduced expression effector cytokines increases immune checkpoint protein levels, inducing dysfunction. RNase1 is positively associated with exhausted gene signatures proteins across...
Abstract Crown ethers are small, cyclic polyethers that have found wide‐spread use in phase‐transfer catalysis and, to a certain degree, protein chemistry. readily bind metallic and organic cations, including positively charged amino acid side chains. We elucidated the crystal structures of several protein‐crown ether co‐crystals grown presence 18‐crown‐6. then employed biophysical methods molecular dynamics simulations compare these complexes with corresponding apoproteins similar...
DNA mimic proteins are unique factors that control the binding activity of target by directly occupying their sites. The extremely divergent amino acid sequences mimics make these hard to predict, and although they likely be ubiquitous, date, only a few have been reported functionally analyzed. Here we used bioinformatic approach look for potential among previously protein structures. From ∼14 candidates, selected Staphylococcus conserved hypothetical SSP0047, proteomic structural approaches...
Acute hepatopancreatic necrosis disease (AHPND) is a newly emergent penaeid shrimp which can cause 70–100% mortality in Penaeus vannamei and monodon, has resulted enormous economic losses since its appearance. AHPND caused by the specific strains of Vibrio parahaemolyticus that harbor pVA1 plasmid express PirAvp PirBvp toxins. These two toxins have been reported to form binary complex. When both are present, they lead death epithelial cells hepatopancreas typical histological symptoms AHPND....
In plants, HEN1-facilitated methylation at 3′ end ribose is a critical step of small-RNA (sRNA) biogenesis. A mutant well-studied Arabidopsis HEN1 (AtHEN1), hen1-1, showed defective developmental phenotype, indicating the importance sRNA methylation. Moreover, Marchantia polymorpha has been identified to have ortholog gene (MpHEN1); however, its function remained unfathomed. Our in vivo and vitro data shown MpHEN1 activity being comparable with AtHEN1, their substrate specificity towards...
Immediate-early proteins from many viruses function as transcriptional regulators and exhibit transactivation activity, DNA binding dimerization. In this study, we investigated these characteristics in white spot syndrome virus (WSSV) immediate-early protein 1 (IE1) attempted to map the corresponding functional domains. Transactivation was by transiently expressing a consisting of domain yeast transactivator GAL4 fused full-length IE1. This GAL4-IE1 fusion successfully activated Autographa...
Vaccinia mature virus requires A26 envelope protein to mediate acid-dependent endocytosis into HeLa cells in which we hypothesized that functions as an acid-sensitive membrane fusion suppressor. Here, provide evidence showing N-terminal domain (aa1-75) of is region regulates fusion. Crystal structure revealed His48 and His53 are close contact with Lys47, Arg57, His314 Arg312, suggesting at low pH these His-cation pairs could initiate conformational changes through protonation subsequent...
Acute hepatopancreatic necrosis disease (AHPND) in shrimp is caused by Vibrio strains that harbor a pVA1-like plasmid containing the pirA and pirB genes. It also known production of PirA PirB proteins, which are key factors drive observed symptoms AHPND, can be influenced environmental conditions this leads to changes virulence bacteria. However, our knowledge, mechanisms involved regulating expression pirA/pirB genes have not previously been investigated. In study, we show AHPND-causing...
Abstract In this proteomic analysis of gills from yellow head virus (YHV)‐infected Penaeus vannamei , we identified 13 spots with up‐regulated protein expression levels and five down‐regulated levels. LC‐nanoESI‐MS/MS indicated that the proteins included enzymes in glycolytic pathway, tricarboxylic acid cycle amino metabolism. The other were arginine kinase, imaginal disk growth factor (IDGF) a Ras‐like GTP binding protein. By contrast, reduced for an SCP‐calcium (SCP), actin‐1,...
The endoglucanase EglA from Piromyces rhizinflata found in cattle stomach belongs to the GH5 family of glycoside hydrolases. crystal structure catalytic domain shows (β/α)8-barrel fold typical enzymes. Adjacent active site EglA, a loop containing disulfide bond not other similar structures may participate substrate binding. Because was blocked by N-terminal His tag neighbouring protein molecule crystal, enzyme–substrate complexes could be obtained soaking but were prepared cocrystallization....
A new clade, Trichoderma formosa, secretes eliciting plant response-like 1 (Epl1), a small peptide elicitor that stimulates immunity. Nicotiana benthamiana pretreated with Epl1 for 3 days developed immunity against Tomato mosaic virus (ToMV) infection. The transcriptome profiles of T. formosa and N. were obtained by deep sequencing; the transcript is 736 nt in length encodes 12-kDa peptide. Identifying critical genes Epl1-mediated was challenging due to high similarity between expression...