A5044661490- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Cellular and Composite Structures
- Aquaculture disease management and microbiota
- Bioinformatics and Genomic Networks
- Mosquito-borne diseases and control
- Computational Drug Discovery Methods
- Studies on Chitinases and Chitosanases
- Gene Regulatory Network Analysis
- Vibration Control and Rheological Fluids
- Protein Structure and Dynamics
- Mechanical Behavior of Composites
- Polymer Foaming and Composites
- DNA Repair Mechanisms
- Characterization and Applications of Magnetic Nanoparticles
- Structural Analysis and Optimization
- Elasticity and Material Modeling
- Extracellular vesicles in disease
- Bacterial biofilms and quorum sensing
- Geomagnetism and Paleomagnetism Studies
- Transportation Safety and Impact Analysis
- Vibrio bacteria research studies
- Surfactants and Colloidal Systems
- Autophagy in Disease and Therapy
- Innovative concrete reinforcement materials
National Cheng Kung University
1998-2023
National Taiwan University
2010-2017
Institute of Zoology
1992-2013
Chung Hua University
2008-2012
National Chung Hsing University
2008
National Central University
2000
Tunghai University
1994
Significance Since 2009, an emergent shrimp disease, acute hepatopancreatic necrosis disease (AHPND), has been causing global losses to the farming industry. The causative agent of AHPND is a specific strain Vibrio parahaemolyticus . We present evidence here that opportunistic V. becomes highly virulent by acquiring unique AHPND-associated plasmid. This virulence plasmid, which encodes binary toxin [ Photorhabdus insect-related toxins (PirA vp and PirB )] induces cell death, stably inherited...
The Warburg effect is an abnormal glycolysis response that associated with cancer cells. Here we present evidence metabolic changes resembling the are induced by a nonmammalian virus. When shrimp were infected white spot syndrome virus (WSSV), in several pathways related to mitochondria. At viral genome replication stage (12 h postinfection [hpi]), glucose consumption and plasma lactate concentration both increased WSSV-infected shrimp, key enzyme of pentose phosphate pathway,...
Background Outbreaks of white spot disease have had a large negative economic impact on cultured shrimp worldwide. However, the pathogenesis causative virus, WSSV (whit syndrome virus), is not yet well understood. enveloped virus. The virion has three structural layers surrounding its core DNA: an outer envelope, tegument and nucleocapsid. In this study, we investigated protein-protein interactions major proteins, including several envelope proteins that are known to be involved in infection...
Abstract For more than three decades, infectious diseases have been one of the major causes economic losses in global shrimp industry. One deadliest pathogens is white spot syndrome virus (WSSV), which mass mortality farmed shrimp. Various aspects WSSV pathogenicity studied, but this review, we will focus only on them, namely metabolic reprogramming. WSSV’s modulation host metabolism affects glycolysis, tricarboxylic acid (TCA) cycle, glutaminolysis and lipid metabolism. Several signaling...
White spot syndrome virus (WSSV) is currently the most serious global threat for cultured shrimp production. Although its large, double-stranded DNA genome has been completely characterized, putative protein functions remain obscure. To provide more informative knowledge about this virus, a proteomic-scale network of WSSV-WSSV interactions was carried out using comprehensive yeast two-hybrid analysis. An array transformants containing each WSSV open reading frame fused with GAL4 binding...
Acute hepatopancreatic necrosis disease (AHPND) in shrimp is caused by Vibrio strains that harbor a pVA1-like plasmid containing the pirA and pirB genes. It also known production of PirA PirB proteins, which are key factors drive observed symptoms AHPND, can be influenced environmental conditions this leads to changes virulence bacteria. However, our knowledge, mechanisms involved regulating expression pirA/pirB genes have not previously been investigated. In study, we show AHPND-causing...
(S)-1-(3-hydroxy-2-phosphonylmethoxypropyl)cytosine (HPMPC), a high-potency antiherpes and anticytomegalovirus (CMV) drug was evaluated in the treatment of experimental retinitis caused by preretinal herpes simplex virus (HSV-1) injection rabbits. HPMPC (100 micrograms/0.1 mL) intravitreally injected 10, 15, 21, 30, or 46 days before, concurrently, 3, 5, 7 after viral inoculation. Ganciclovir (200 7, 10 before HSV-1 inoculation, concurrent with Eyes pretreated were protected from for 15-21...
In this study we identified viral gene targets of the important redox regulator thioredoxin (Trx), and explored in depth how Trx interacts with immediate early #1 (IE1) white spot syndrome virus (WSSV).In a pull-down assay, found that recombinant bound to IE1 under oxidizing conditions, coimmunoprecipitation assay showed WSSV when transfected cells were subjected oxidative stress. A mutants no binding occurred cysteine 62 was replaced by serine. Electrophoretic mobility shift (EMSA) DNA...
Members of the microRNA miR-10 family are highly conserved and play many important roles in diverse biological mechanisms, including immune-related responses cancer-related processes certain types cancer. In this study, we found most upregulated shrimp from Penaeus vannamei during white spot syndrome virus (WSSV) infection was miR-10a. After confirming expression level miR-10a by northern blot quantitative RT-PCR, an vivo experiment showed that viral copy number decreased miR-10a-inhibited...
White spot syndrome virus (WSSV) is a very large dsDNA virus. The accepted shape of the WSSV virion has been as ellipsoidal, with tail-like extension. However, due to scarcity reliable references, pathogenesis and morphogenesis are not well understood. Here, we used transmission electron microscopy (TEM) cryogenic (Cryo-EM) address some knowledge gaps. We concluded that mature virions stout oval-like do have extensions. Furthermore, there were two distinct ends in nucleocapsids: portal cap...
Shrimp white spot disease (WSD), which is caused by syndrome virus (WSSV), one of the world's most serious shrimp diseases. Our objective in this study was to use an immunomagnetic reduction (IMR) assay develop a highly sensitive, automatic WSSV detection platform targeted against ICP11 (the expressed protein). After characterizing magnetic reagents (Fe3O4 nanoparticles coated with anti ICP11), limit for protein using IMR approximately 2 x 10−3 ng/ml, and linear dynamic range 0.1~1 106...
Yeast cell cycle Boolean network was used as a case study of robustness to protein noise. Robustness interpreted involving stability G1 steady state and sequence gene expression from START stationary G1. A measure evaluate strength proposed. Robust putative networks corresponding the same wild‐type were sampled. Architecture yeast can be revealed by average topology profile sampled robust networks.
Chromatid exchanges and chromatid breaks were synergistically increased by a 2-h post-treatment with arsenite (VS treatment) but not arabinofuranosyl cytosine (VA of UV-irradiated late-G1 Chinese hamster ovary cells. In order to determine the mechanism this UV-arsenite coclastogenicity, we have compared effects on generation DNA strand in cells alkaline elution sucrose sedimentation. Only very small numbers detected immediately after VS treatment, however parental strands as reached mitosis...
AbstractIt has previously been reported that chromosome aberrations induced by ultraviolet (UV) radiation can be enhanced treatment with sodium arsenite for 24 h post-irradiation. Using synchronized CHO-K1 cells, it now established cells in the transitional stage from late G1 to early S phase are most vulnerable coclastogenic effect of UV and arsenite. This result cannot explained special vulnerability transition clastogenicity, as effects caffeine or arabinofuranosylcytosine were detected...