A5091272022- Aquaculture disease management and microbiota
- Invertebrate Immune Response Mechanisms
- Animal Virus Infections Studies
- Insect symbiosis and bacterial influences
- Aquaculture Nutrition and Growth
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Insect Resistance and Genetics
- vaccines and immunoinformatics approaches
- Studies on Chitinases and Chitosanases
- Neurobiology and Insect Physiology Research
- Plant Virus Research Studies
- Microbial infections and disease research
- Vibrio bacteria research studies
- Crustacean biology and ecology
- interferon and immune responses
- Bacteriophages and microbial interactions
- Autophagy in Disease and Therapy
- Essential Oils and Antimicrobial Activity
- Piperaceae Chemical and Biological Studies
- Aquatic life and conservation
- Cell death mechanisms and regulation
- Ubiquitin and proteasome pathways
- Biosensors and Analytical Detection
- Virology and Viral Diseases
National Science and Technology Development Agency
2014-2024
National Center for Genetic Engineering and Biotechnology
2014-2024
Suan Sunandha Rajabhat University
2020
Chulalongkorn University
2014-2015
Mahidol University
2006-2014
RNA interference (RNAi) is a specific and effective approach for inhibiting viral replication by introducing double-stranded (ds)RNA targeting the gene. In this study, we employed combinatorial to interfere multiple gene functions of white spot syndrome virus (WSSV), most lethal shrimp virus, using single-batch dsRNA, so-called "multi-WSSV dsRNA." A co-cultivation RNase-deficient E. coli was developed produce dsRNA major structural protein (VP28) hub (WSSV051) with high number interacting...
The gene of RNA viruses, encoding RNA-directed polymerase (RdRp) is relatively conserved due to its crucial function in viral genome replication and transcription making it a useful target for genetic diversity study PCR detection. In this study, we investigated the 21 tilapia lake virus (TiLV) segment 1 sequences predictively coding RdRp subunit P1. Those were obtained from infected fish samples collected Ecuador, Israel, Peru, Thailand between 2011 2019 (nine 12 GenBank). Primers then...
Tilapia lake virus (TiLV) is an emerging that rapidly spreading across the world. Over past 6 years (2014–2020), TiLV outbreaks had been reported in at least 16 countries, spanning three continents, including Asia, Africa, and America. Despite its enormous economic impact, origin, evolution epidemiology are still largely poorly characterized. Here, we report eight whole-genome sequences from Thailand sampled between 2014 2019. Together with publicly available various regions of world,...
White spot syndrome virus (WSSV) is currently the most serious global threat for cultured shrimp production. Although its large, double-stranded DNA genome has been completely characterized, putative protein functions remain obscure. To provide more informative knowledge about this virus, a proteomic-scale network of WSSV-WSSV interactions was carried out using comprehensive yeast two-hybrid analysis. An array transformants containing each WSSV open reading frame fused with GAL4 binding...
Melanization mediated by the prophenoloxidase (proPO) activating system is a rapid immune response used invertebrates against intruding pathogens. Several masquerade-like and serine proteinase homologues (SPHs) have been demonstrated to play an essential role in proPO activation insects crustaceans. In previous study, we characterized SPH, PmMasSPH1, black tiger shrimp Penaeus monodon as multifunctional protein based on its recognition antimicrobial activity Gram-negative bacteria Vibrio...
Long pepper ( Piper retrofractum Vahl) is a Thai medicinal herb which has been used as one of the common ingredients in variety foods. Here, we investigated antimicrobial activities crude bioactive metabolites extracted from fruits P. against 10 pathogenic organisms (bacteria and yeast) causing opportunistic infections human or animals including Bacillus subtilis ATCC6633, Staphylococcus aureus ATCC25923, Enterococcus faecalis ATCC2921, Escherichia coli ATCC25922, Klebsiella pneumonia...
White spot syndrome virus (WSSV) is one of the most serious pathogens penaeid shrimp. Although its genome has been completely characterized, functions putative proteins are not yet known. It suggested that major nucleocapsid protein VP15 involved in packaging WSSV during virion formation. However, little known relationship with shrimp host cells. Using yeast two-hybrid approach to screen a lymphoid organ (LO) cDNA library for might interact VP15, named PmFKBP46 was identified. had high...
DAO Diseases of Aquatic Organisms Contact the journal Facebook Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout JournalEditorsSpecials 117:133-143 (2015) - DOI: https://doi.org/10.3354/dao02942 Shewanella putrefaciens in cultured tilapia detected by a new calcein-loop-mediated isothermal amplification (Ca-LAMP) method Rungkarn Suebsing1, Jantana Kampeera1, Sarawut Sirithammajak1, Padmaja Jayaprasad Pradeep3, Sarocha Jitrakorn2,3, Narong Arunrut1,...
White spot syndrome virus (WSSV) presents a considerable peril to the aquaculture sector, leading notable financial consequences on global scale. Previous studies have identified hub proteins, including WSSV051 and WSSV517, as essential binding elements in protein interaction network of WSSV. This work further investigates functional structures potential applications WSSV complexes managing infection. Using computational methodologies, we successfully generated comprehensive...
Tilapia lake virus (TiLV) is an emerging that rapidly spreading across the world. Over past 6 years (2014–2020), TiLV outbreaks had been reported in at least 16 countries, spanning three continents, including Asia, Africa, and America. Despite its enormous economic impact, origin, evolution, epidemiology are still largely poorly characterised. Here, we report eight whole genome sequences from Thailand sampled between 2014–2019. Together with publicly available various regions of world,...