A5066133791- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Chronic Lymphocytic Leukemia Research
- Metabolomics and Mass Spectrometry Studies
- Advanced Proteomics Techniques and Applications
- Mass Spectrometry Techniques and Applications
- Analytical Chemistry and Chromatography
- Advanced Battery Materials and Technologies
- Advancements in Battery Materials
- Advanced Chemical Sensor Technologies
- Peptidase Inhibition and Analysis
- RNA modifications and cancer
- Caveolin-1 and cellular processes
- Monoclonal and Polyclonal Antibodies Research
- Cell Adhesion Molecules Research
- Cancer Cells and Metastasis
- Extraction and Separation Processes
- Immunotherapy and Immune Responses
- Cancer Research and Treatments
- Genomics, phytochemicals, and oxidative stress
- Antimicrobial Peptides and Activities
- Meat and Animal Product Quality
- Plant Virus Research Studies
- Sarcoma Diagnosis and Treatment
- Cardiovascular Function and Risk Factors
National Taiwan University
2018-2025
National Tsing Hua University
2020-2023
Institute of Chemistry, Academia Sinica
2010-2022
Advanced Semiconductor Engineering (Taiwan)
2022
National Taipei University
2022
Hsing Wu University
2021
National University of Tainan
2019
Chang Gung University
2017
Linkou Chang Gung Memorial Hospital
2017
National Cheng Kung University
2006-2015
Protein phosphorylation catalyzed by kinases plays crucial roles in regulating a variety of intracellular processes. Owing to an increasing number vivo sites that have been identified mass spectrometry (MS)-based proteomics, the RegPhos, available online at http://csb.cse.yzu.edu.tw/RegPhos2/, was developed explore protein networks human. In this update, we not only enhance data content human but also investigate kinase–substrate mouse and rat. The experimentally validated as well their...
A synergy between β-amyloid (Aβ) and tau appears to occur in Alzheimer disease (AD), but the mechanisms of interaction, potential locations, are little understood. This study investigates possibility such interactions within cortical synaptic compartments APP/PS1 mice. We used label-free quantitative mass spectrometry phosphoproteome synaptosomes, covering 2400 phosphopeptides providing an unbiased survey phosphorylation changes associated with amyloid pathology. Hyperphosphorylation was...
Core 1 β1,3-galactosyltransferase (C1GALT1) controls the crucial step of GalNAc-type O-glycosylation and is overexpressed in various human malignancies. However, its role head neck squamous cell carcinoma (HNSCC) remains unclear. Here we demonstrate that C1GALT1 expression upregulated HNSCC tumors associated with adverse clinicopathologic features. Moreover, high predicts poor disease-free overall survivals. overexpression enhances viability, migration, invasion, which can be reversed by...
Atherosclerosis preferentially develops in arterial branches and curvatures where vascular endothelium is exposed to disturbed flow. In this study, the effects of flow on regulation endothelial phosphoproteins their contribution therapeutic application atherogenesis were elucidated.
Objectives/Hypothesis The correlation between incidental parathyroidectomy (IP) during thyroidectomy and postoperative hypocalcemia remains controversial. Our aim was to investigate the incidence of IP, risk factors, impact on patient outcomes. Study Design Retrospective cohort study. Methods This a retrospective observational study including 3,186 consecutive patients who underwent January 2007 December 2014. were divided into two groups: IP group non‐IP. Numerous clinical parameters...
G protein-coupled estrogen receptor-1 (GPER), a member of the receptor (GPCR) superfamily, mediates estrogen-induced proliferation normal and malignant breast epithelial cells. However, its role in cancer stem cells (BCSCs) remains unclear. Here we showed greater expression GPER BCSCs than non-BCSCs three patient-derived xenografts ER
Abstract Crosslinking of B-cell receptor (BCR) sets off an apoptosis programme, but the underlying pathways remain obscure. Here we decipher molecular mechanisms bridging activation and mediated by post-translational modification (PTM). We find that O -GlcNAcase inhibition enhances induced BCR crosslinking. This proteome-scale analysis functional interplay between protein -GlcNAcylation phosphorylation in stimulated mouse primary B cells identifies 313 -GlcNAcylation-dependent phosphosites...
The current approaches remain insufficient for measuring chicken egg spoilage or present analytical limitations. This study aimed to complement the existing analyses and identify novel markers using liquid chromatography-high resolution mass spectrometry-based foodomics strategies. In discovery set, comparative untargeted metabolomics was utilized marker candidates in microbially inoculated eggs. Markers were annotated by spectral matching with authentic standards, experimental libraries,...
In recent electronic product market, high density chiplet package integration is taking leads to the world of "more than Moore". Since breaking down from a large chip, multi-chip architectures provide performance and low cost production. These packages are developed for server, computing, router, switcher markets. Therefore, bandwidth memory (HBM) devices or multiple ASIC dies requires I/O counts signal transmitting. For this requirement, chip last fanout on substrate (CL-FOCoS) technology...
Helper-component proteinase (HC-Pro), encoded by potyviruses, function as viral suppressors of RNA silencing (VSRs). Despite their conserved role, HC-Pros share approximately 40% similarity, implying potential differences in VSR efficiency, particularly ability to inhibit HEN1 methyltransferase activity. This study investigated the inhibitory from different potyviruses transgenic plants. P1/HC-Pro turnip mosaic virus (P1/HC-ProTu) exhibited most potent inhibition HEN1, followed zucchini...
Pleural effusion, an accumulation of pleural fluid, contains proteins originating from plasma filtrate and, especially when tissues are damaged, parenchymal interstitial spaces lungs and/or other organs. This report presents data the first global proteomic analysis human effusion. A composite sample was prepared by pooling effusions seven lung adenocarcinoma patients. Two-dimensional gel electrophoresis revealed 472 silver-stained spots. 242 selected spots were subjected to protein...
S-glutathionylation, the covalent attachment of a glutathione (GSH) to sulfur atom cysteine, is selective and reversible protein post-translational modification (PTM) that regulates activity, localization, stability. Despite its implication in regulation functions cell signaling, substrate specificity cysteine S-glutathionylation remains unknown. Based on total 1783 experimentally identified sites from mouse macrophages, this work presents an informatics investigation including structural...
Alzheimer's disease (AD) is the most common cause of dementia in late life. It difficult to precisely diagnose AD at early stages, making biomarker search essential for further developments. The objective this study was identify protein biomarkers associated with aluminum ions toxicity (AD-like toxicity) a human neuroblastoma cell model, SH-SY5Y and assess potential prevention by NAP (NAPVSIPQ). Complete proteomic techniques were implemented. Four proteins identified as up-regulated ion...
Cells in the tumor microenvironment (TME) communicate via membrane‐bound and secreted proteins, which are mostly glycosylated. Altered glycomes of malignant tumors influence behaviors stromal cells. In this study, we showed that loss core‐1 β1,3‐galactosyltransferase (C1GALT1)‐mediated O‐glycosylation suppressed growth syngeneic head neck cancer mouse models. O‐glycan truncation cells promoted M1 polarization macrophages, enhanced T‐cell‐mediated cytotoxicity, reduced interleukin‐6 (IL‐6)...
Spinel Li<sub>4</sub>Ti<sub>5</sub>O<sub>12</sub> nanosheets prepared by hydrothermal method were used as anode materials for lithium ion batteries.
Secreted proteins, referred to as the secretome, are known regulate a variety of biological functions and involved in multitude pathological processes. However, some secreted proteins from cell cultures difficult detect because their intrinsic low abundance. They frequently masked by shed lysed cells substantial amounts serum used culture medium. We have proposed an analytical platform for sensitive detection utilizing hollow fiber (HFC) system coupled with proteomic approaches. The HFC...
Abstract Background Posttranscriptional gene silencing (PTGS) is one of the most important mechanisms for plants during viral infection. However, viruses have also developed suppressors to negatively control PTGS by inhibiting microRNA (miRNA) and short-interfering RNA (siRNA) regulation in plants. The first identified suppressor, P1/HC-Pro, a fusion protein that was translated from potyviral RNA. Upon infecting plants, P1 itself released HC-Pro self-cleaving activity P1. has an unknown...
Protein phosphorylation is a key post-translational modification that governs biological processes. Despite the fact number of analytical strategies have been exploited for characterization protein phosphorylation, identification sites still challenging. We proposed here an alternative approach to mine phosphopeptide signals generated from mixture proteins when liquid chromatography−tandem mass spectrometry (LC−MS/MS) analysis involved. The combined dephosphorylation reaction, accurate...
Lung cancer, one of the most common causes cancer-related death worldwide, has been associated with high treatment cost and imposed great burdens. The 5-year postoperative survival rate lung cancer (13%) is lower than many other leading cancers indicating urgent needs to dissect its pathogenic mechanisms discover specific biomarkers. Although several proteins have proposed be potential candidates for diagnosis they present low accuracy in clinical settings. Metabolomics thus emerged as a...