Staphylococcus aureus causes many diseases in humans, ranging from mild skin infections to serious, life-threatening, superantigen-mediated Toxic Shock Syndrome (TSS). S. may be asymptomatically carried the anterior nares or vagina on skin, serving as a reservoir for infection. Pulsed-field gel electrophoresis clonal type USA200 is most widely disseminated colonizer and leading cause of TSS. The cytolysin α-toxin (also known α-hemolysin Hla) major epithelial proinflammatory exotoxin produced...

Abstract The maturation process of lymphocyte was related to many blood diseases, such as lymphoma and lymphoid leukemia. Many TFs miRNAs were separately studied in the development B T cells. In this study, we aim discover TF miRNA co-regulation identify key regulators cells maturation. We obtained candidate genes, for each stage their maturation, then constructed TF-miRNA-gene feed-forward loops (FFLs) by our previous methods. Statistical test FFLs indicated enrichment significance....

ABSTRACT We investigated the effects of glycerol monolaurate (GML) on Lactobacillus , Candida and Gardnerella vaginalis human vaginal microflora. Our previous work demonstrated that 6 months GML treatment vaginally does not alter lactobacillus counts in monkeys. G. are commonly associated with infections women, many becoming chronic or recurrent. In vitro growth inhibition studies determined (0 to 500 μg/ml) against multiple species . A randomized, double-blind study microflora colonized...

The unique geographic features of Taiwan are attributed to the rich indigenous and endemic plant species in Taiwan. These plants serve as resourceful bank for biologically active phytochemicals. Given that these plant‐derived chemicals prototypes potential drugs diseases, databases connecting chemical structures pharmacological activities may facilitate drug development. To enhance utility data, it is desirable develop a database compounds corresponding from A anticancer, antiplatelet,...

Myocardial infarction (MI) is a leading cause of death in the world and many genes are involved it. Transcription factor (TFs) microRNAs (miRNAs) key regulators gene expression. We hypothesized that miRNAs TFs might play combinatory regulatory roles MI. After collecting MI candidate from various resources, we constructed comprehensive MI-specific miRNA-TF co-regulatory network by integrating predicted experimentally validated TF miRNA targets. found some hub nodes (e.g. miR-16 miR-26) this...

The current approaches remain insufficient for measuring chicken egg spoilage or present analytical limitations. This study aimed to complement the existing analyses and identify novel markers using liquid chromatography-high resolution mass spectrometry-based foodomics strategies. In discovery set, comparative untargeted metabolomics was utilized marker candidates in microbially inoculated eggs. Markers were annotated by spectral matching with authentic standards, experimental libraries,...

The aim of this study was to design oil in water (O/W) microemulsion formulations for the topical administration azelaic acid. permeability acid through rat skin and anti-inflammatory activities were conducted examine efficacy designed formulations. Skin irritation stability tests also performed. significantly increased by using O/W microemulsions as carriers. edema index ear swelling percentage recovered 5% drug-loaded formulation a 20% commercial product, demonstrating that experimental...

Staphylococcus aureus is a major human pathogen capable of causing various diseases, from skin infections to life-threatening pneumonia and toxic shock syndrome. S. exoproteins, including superantigens, contribute significantly the pathogenesis this organism. Antibiotics inhibit growth, but often provide no protection exoproteins. With emergence antibiotic-resistant aureus, new therapeutic options treat or prevent aureus-associated diseases are critical. Most begin on mucosal surfaces direct...

Glycerol monolaurate (GML), a 12 carbon fatty acid monoester, inhibits Staphylococcus aureus growth and exotoxin production, but is degraded by S. lipase. Therefore, dodecylglycerol (DDG), monoether, was compared in vitro vivo to GML for its effects on growth, stability.Antimicrobial of DDG (0 500 microg/ml) 54 clinical isolates aureus, including pulsed-field gel electrophoresis (PFGE) types USA200, USA300, USA400, were determined vitro. A rabbit Wiffle ball infection model assessed (1 mg/ml...

Staphylococcus aureus is a major cause of infective endocarditis (IE) and sepsis. In addition, 50% IE survivors develop strokes metastatic abscesses due to the release emboli from infected valves. Both methicillin-resistant (MRSA) methicillin-sensitive (MSSA) strains sepsis, they may be categorized by pulsed-field gel electrophoresis, for example clonal types USA200, 300, 400. We hypothesize that secreted virulence factors contribute their differential ability and/or Rabbits are an excellent...

Background . Kawasaki disease (KD) is characterized by systemic vasculitis of unknown etiology. Our previous studies showed expression CD40 ligand on CD4+ T cells correlated to the coronary artery lesion (CAL) and progress in KD. Other from Japan suggested role CD40L pathogenesis CAL, this might help explain excessive number males affected with KD but cannot be reproduced Taiwanese population. This study was conducted investigate polymorphism CAL formation. Methods A total 950 subjects (381...

Abstract Cutaneous wounds are prompt to be contaminated by bacteria, but the clinical benefits of applying antibiotics and antiseptics in wound management have not been proven. Statins 3‐hydroxy‐3‐methylglutaryl‐coenzyme A ( HMG‐CoA ) reductase inhibitors commonly used lower cholesterol levels. Studies indicated that statins, especially simvastatin, promote healing experimental models. As Staphylococcus aureus is one most important microorganism responsible for infections, aims this study...

Glycine N-methyltransferase (GNMT) expression is vastly downregulated in hepatocellular carcinomas (HCC). High rates of GNMT knockout mice developed HCC, while overexpression prevented aflatoxin-induced carcinogenicity and inhibited liver cancer cell proliferation. Therefore, this study, we aimed for the identification a inducer HCC therapy. We established promoter-driven luciferase reporter assay as drug screening platform. Screening 324 pure compounds 480 crude extracts from Chinese...

P-glycoprotein (PGP) is a polymorphic efflux transporter located on the blood brain barrier that potentially affects penetration of atypical antipsychotics into central nervous system. Increased antipsychotic to primary site activity may result in greater symptom improvement or occurrence side effects. This investigation examined relationship between three common PGP polymorphisms (C1236T, G2677TA, and C3435T) response 6 weeks open-label olanzapine treatment patients with schizophrenia....

Abstract Numerical and experimental studies of the transient response concrete shafts subjected to elastic impact were carried out using finite element method impact-echo testing technique. Two- three-dimensional performed for: (a) solid shafts; (b) containing cracks, voids, layers low-quality concrete, changes in cross section, such as bulges necks; (c) soil. These gain an improved understanding flaws, problems for which there are currently no analytical solutions. It was also intent...

Pulsed-field gel electrophoresis (PFGE) clonal type USA200 is the most widely disseminated Staphylococcus aureus colonizer of nose and a major cause toxic shock syndrome (TSS). Exoproteins derived from these organisms have been suggested to contribute their colonization causation human diseases but not well-characterized. Two representative S. isolates, MNPE (α-toxin positive) CDC587 mutant), isolated pulmonary post-influenza TSS menstrual vaginal TSS, respectively, were evaluated....

The rich indigenous and endemic plants in Taiwan serve as a resourceful bank for biologically active phytochemicals. Based on our TIPdb database curating bioactive phytochemicals from plants, this study presents three-dimensional (3D) chemical structure named TIPdb-3D to support the discovery of novel pharmacologically compounds. Merck Molecular Force Field (MMFF94) was used generate 3D structures TIPdb. could facilitate analysis quantitative structure–activity relationship, exploration...

Southern China is a major rice-producing region where mixed rice cropping systems (single (SCR) and double-cropping (DCR)) are widely practiced. An accurate estimation of the spatiotemporal changes in important for understanding dynamic paddy system developing suitable agricultural policies. However, few studies focus on mapping long-term this subtropical region. The objective research 1) to improve phenology- pixel-based (PPPM) algorithm by simultaneously considering phenology signatures...