A5047171658- Metabolomics and Mass Spectrometry Studies
- Mass Spectrometry Techniques and Applications
- Effects and risks of endocrine disrupting chemicals
- Advanced Proteomics Techniques and Applications
- Toxic Organic Pollutants Impact
- Analytical Chemistry and Chromatography
- Carcinogens and Genotoxicity Assessment
- Peptidase Inhibition and Analysis
- Advanced Chemical Sensor Technologies
- Health, Environment, Cognitive Aging
- Glycosylation and Glycoproteins Research
- Microplastics and Plastic Pollution
- Tea Polyphenols and Effects
- Neurotransmitter Receptor Influence on Behavior
- Advanced biosensing and bioanalysis techniques
- Air Quality and Health Impacts
- Protease and Inhibitor Mechanisms
- Microfluidic and Capillary Electrophoresis Applications
- Hormonal and reproductive studies
- Galectins and Cancer Biology
- RNA and protein synthesis mechanisms
- Dialysis and Renal Disease Management
- Ion-surface interactions and analysis
- Machine Learning in Bioinformatics
- Extracellular vesicles in disease
National Cheng Kung University
2016-2025
Taiwan Society of Thrombosis and Hemostasis
2017
National Taiwan University of Science and Technology
2014
National Yang Ming Chiao Tung University
2009-2010
Institute of Chemistry, Academia Sinica
2010
Institute of Information Science, Academia Sinica
2010
Victoria University of Wellington
2010
Chang Gung University
2009
Chang Gung Memorial Hospital
2009
University of California, Los Angeles
2009
Maternal hypothyroidism during pregnancy can cause adverse effects in the fetus. Scientific evidence has shown that probable thyroid-like function of some phthalates vitro and vivo, exposure, begin utero. This study investigated association between phthalate exposure thyroid hormones pregnant women. Serum spot urine samples were collected from 76 Taiwanese women at second trimester. Thyroid hormones, including thyroid-stimulating hormone (TSH), triiodothyronine (T3), thyroxine (T4) free T4...
Plants synthesize the osmoprotectant glycine betaine via route choline → aldehyde betaine. In spinach, first step is catalyzed by monooxygenase (CMO), a ferredoxin-dependent stromal enzyme that has been hypothesized to be an oligomer of identical subunits and Fe-S protein. Analysis HPLC matrix-assisted laser desorption ionization MS confirmed native CMO contains only one type subunit ( M r 42,864). Determination acid-labile sulfur nonheme iron demonstrated there [2Fe-2S] cluster per subunit,...
Background: Few studies have shown an association between prenatal phthalate exposure and adverse effects on neurodevelopment behavior in young children.Objectives: We aimed to assess the relationship esters syndromes children at 8 years of age.Methods: A total 122 mother–child pairs from general population central Taiwan were studied 2000 2009. Mono-methyl (MMP), mono-ethyl (MEP), mono-butyl (MBP), mono-benzyl (MBzP), three di-(2-ethylhexyl) (DEHP) metabolites—mono-2-ethylhexyl,...
BACKGROUNDPhthalates are known to have estrogenic effects in cell models and experimental animals. However, the evidence regarding of phthalates on human reproduction is still limited. We conducted a case–control study determine whether estrogen-dependent diseases associated with phthalate exposure how glutathione S-transferase M1 (GSTM1; major detoxification enzyme) genotype modulates risk.
The inhibitor protein (IP) that inactivates spinach leaf NADH:nitrate reductase (NR) has been identified for the first time as a member of eukaryotic 14‐3‐3 family based on three lines evidence. First, sequence an eight amino acid tryptic peptide, obtained from immunopurified IP, matched highly conserved region proteins. Second, authentic family, recombinant Arabidopsis GF14ω, caused inactivation phospho‐NR in magnesium‐dependent manner identical to IP. Third, anti‐GF14 monoclonal antibody...
Abstract Experiments were conducted to determine whether sucrose synthase (SuSy) was phosphorylated in the elongation zone of maize (Zea mays L.) leaves. The approximately 90-kD subunit SuSy 32P-labeled on seryl residue(s) when excised shoots fed [32P]orthophosphate. Both isoforms (the SS1 and SS2 proteins) vivo, tryptic peptide-mapping analysis suggested a single, similar phosphorylation site both proteins. A combination matrix-assisted laser desorption/ionization time-of-flight mass...
Canine distemper (CD) is one of the most contagious and lethal viral diseases in dogs. Despite widespread use vaccines, prevalence CD virus (CDV) has increased at an alarming rate recent years. In this phylodynamic study, we investigated spatiotemporal modes dispersal, demographic trends, effectiveness vaccines for CDV. A total 188 full-length CDV hemagglutinin (H) gene sequences dataset were subjected to recombination analysis, including seven from modified live vaccine (MLV) strains 12...
In cancer metastasis, secreted proteins play an important role in promoting cell migration and invasion thus also the increase of metastasis extracellular microenvironment. this study, we developed a strategy that combined simple gel-aided protein purification with iTRAQ labeling to quantify discover metastasis-associated lung secretome. Secreted associated were produced using CL1−0 CL1−5 cells different metastatic abilities. Quantitative secretomics analysis identified total 353 proteins, 7...
Metastasis is a major obstacle that must be overcome for the successful treatment of lung cancer. Proteins secreted by cancer cells may facilitate progression metastasis, particularly within phases migration and invasion. To discover metastasis-promoting secretory proteins cells, we used label-free quantitative proteomics approach compared secretomes from adenocarcinoma cell lines CL1-0 CL1-5, which exhibit low high metastatic properties, respectively. By employing analyses, identified 660...
Nasopharyngeal carcinoma (NPC) is one of the most common malignant tumors in Southeast Asia. Unfortunately, NPC victims have had metastasis when first diagnosed due to its deep location and vague symptoms. To date, discovery sensitive specific biomarkers for improving detection remains a challenge. Our previous study established strategy cell secretome analysis using hollow fiber culture (HFC) system combined with liquid chromatography mass spectrometry. Herein, above platform was used...
Abstract The relative signal intensities of [M + H] vs Na] ions some small peptides were found to be highly matrix‐dependent in matrix‐assisted laser desorption/ionization (MALDI) experiments when sinapinic acid, α‐cyano‐4‐hydroxycinnamic acid and 2,5‐dihydroxybenzoic used as matrices. Presumably, this observation results from the competition two different mechanisms for ionization steps. Possible formations are proposed discussed pursuit defining step MALDI. Several designed performed...
DNA polymerase α-primase is known to be phosphorylated in human and yeast cells a cell cycle-dependent manner on the p180 p68 subunits. Here we show that phosphorylation of purified by cyclin A/cdk2 vitro reduced its ability initiate simian virus 40 (SV40) replication vitro, while E/cdk2 stimulated initiation activity. Tryptic phosphopeptide mapping revealed family peptides was modified well poorly E/cdk2. The phosphopeptides were identical with both kinases. By mass spectrometry, peptide...
Pleural effusion, an accumulation of pleural fluid, contains proteins originated from plasma filtrate and, especially when tissues are damaged, parenchyma interstitial spaces lungs and/or other organs. This study details protein profiles in human effusion 43 lung adenocarcinoma patients by a two-dimensional nano-high performance liquid chromatography electrospray ionization tandem mass spectrometry (2D nano-HPLC-ESI-MS/MS) system. The experimental results revealed the identification 1415...
Nonsmall cell lung cancer (NSCLC) is the most common type of cancer, which one prominent causes cancer-related mortality worldwide. Discovery serum tumor markers could facilitate early NSCLC detection and metastatic prognosis. Here, we simultaneously analyzed secretome proteomic profiles pleural effusion from adenocarcinoma patients for biomarker discovery. Retinoblastoma-associated binding protein 46 (RbAp46), proteins detected both in proteome, was chosen further evaluation. Both RbAp46...