A5073773690- Advanced X-ray Imaging Techniques
- X-ray Spectroscopy and Fluorescence Analysis
- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Advanced biosensing and bioanalysis techniques
- Advanced Electron Microscopy Techniques and Applications
- Trace Elements in Health
- Bacteriophages and microbial interactions
- Monoclonal and Polyclonal Antibodies Research
- Medical Imaging Techniques and Applications
- Electron and X-Ray Spectroscopy Techniques
- Plant pathogens and resistance mechanisms
- Legume Nitrogen Fixing Symbiosis
- Plant Physiology and Cultivation Studies
- Nuclear Physics and Applications
- Metal and Thin Film Mechanics
- Electrochemical Analysis and Applications
- Astrophysical Phenomena and Observations
- Enzyme Structure and Function
- Nanoplatforms for cancer theranostics
- Planetary Science and Exploration
- 3D Printing in Biomedical Research
- Biosensors and Analytical Detection
- Diamond and Carbon-based Materials Research
- Molecular Biology Techniques and Applications
Northwestern University
2013-2024
Anhui Medical University
2023-2024
Argonne National Laboratory
2007-2023
Sichuan University
2022
Henan Academy of Agricultural Sciences
2016
Zhoukou City Academy of Agricultural Sciences
2016
Shijiazhuang Academy of Agriculture and Forestry Sciences
2016
Luohe Academy of Agricultural Sciences
2016
Anyang Academy of Agricultural Sciences
2016
Utah State University
2012
Bacterial surface appendages called pili and needle-like filaments are associated with protein and/or DNA transfer to recipient plant, human, or bacterial cells during pathogenesis conjugation. Although it has long been suspected that function as a conduit for transfer, direct evidence lacking. The Hrp pilus of Pseudomonas syringae is assembled by the type III secretion system. We used an in situ immunogold labeling procedure visualize extrusion effector protein, AvrPto, from tip pilus,...
The NPR1 gene plays a pivotal role in systemic acquired resistance plants. Its overexpression Arabidopsis and rice results increased disease elevated expression of pathogenesis-related (PR) genes. An homolog, MpNPR1-1, was cloned from apple (Malus x domestica) overexpressed two important cultivars, Galaxy M26. Apple leaf pieces were transformed with the MpNPR1 cDNA under control inducible Pin2 or constitutive Cauliflower mosaic virus (CaMV)35S promoter using Agrobacterium tumefaciens....
Significance X-ray fluorescence microscopy provides unparalleled sensitivity for measuring the distribution of trace elements in many-micrometer-thick specimens, whereas ptychography offers a path to imaging weakly fluorescing biological ultrastructure at beyond-focusing-optic resolution. We demonstrate here first time, our knowledge, combination and frozen-hydrated specimens cryogenic temperatures, with excellent structural chemical preservation. This combined approach will have significant...
Hard X-ray fluorescence microscopy is one of the most sensitive techniques for performing trace elemental analysis biological samples such as whole cells and tissues. Conventional sample preparation methods usually involve dehydration, which removes cellular water may consequently cause structural collapse, or invasive processes embedding. Radiation-induced artifacts also become an issue, particularly spatial resolution increases beyond sub-micrometer scale. To allow imaging under hydrated...
Sequestration within the cytoplasm often limits efficacy of therapeutic nanoparticles that have specific subcellular targets. To allow for both cellular and nanoparticle delivery, we created epidermal growth factor receptor (EGFR)-targeted Fe3O4@TiO2 use native intracellular trafficking EGFR to improve internalization nuclear translocation in EGFR-expressing HeLa cells. While bound EGFR, these do not interfere with interaction between karyopherin-β, a protein is critical ligand-bound...
X-ray microscopy can be used to image whole, unsectioned cells in their native hydrated state. It complements the higher resolution of electron for submicrometer thick specimens, and molecule-specific imaging capabilites fluorescence light microscopy. We describe here first use fast, continuous x-ray scanning frozen simultaneous sub-20 nm ptychographic transmission with high contrast, sub-100 deconvolved diffusible bound ions at concentrations, without need add specific labels. By working...
Accurate knowledge of elemental distributions within biological organisms is critical for understanding their cellular roles. The ability to couple this with overall architecture in three dimensions (3D) deepens our chemistry. Using a whole, frozen-hydrated Chlamydomonas reinhardtii cell as an example, we report the development 3D correlative microscopy through combination simultaneous cryogenic x-ray ptychography and fluorescence microscopy. By taking advantage recently developed...
Summary Trace metals play important roles in biological function, and x‐ray fluorescence microscopy (XFM) provides a way to quantitatively image their distribution within cells. The faithfulness of these measurements is dependent on proper sample preparation. Using mouse embryonic fibroblast NIH/3T3 cells as an example, we compare various approaches the preparation adherent mammalian for XFM imaging under ambient temperature. Direct side‐by‐side comparison shows that plunge‐freezing‐based...
Bacterial surface appendages called pili often are associated with DNA and/or protein transfer between cells. The exact function of in the process is not understood and a matter considerable debate. Hrp pilus assembled by type III secretion system Pseudomonas syringae pv. tomato ( Pst ) strain DC3000. In this study, we show that hrpA gene, which encodes major subunit pilus, required for putative virulence proteins, such as HrpW AvrPto. addition, gene full expression genes encode regulatory,...
Pili are required for protein and/or DNA transfer from bacteria to recipient plant or bacterial cells, based on genetic evidence. However, it has never been shown directly that the effector proteins localized along inside pili in situ . Failure visualize an association of proteins/DNA with is central issue debate regarding exact function and transfer. In this study, a newly developed immunogold labelling procedure enabled visualization specific localization type III Erwinia amylovora...
Scanning fluorescence X-ray microscopy lets one non-destructively and quantitatively map the distribution of most biologically-important metals in cells tissues. For studies on large-scale tissues organs, a spatial resolution several micrometers is often sufficient; this case, bending magnets at synchrotron light sources provide abundant flux. We describe here use magnet beamline 8-BM-B Advanced Photon Source (APS) with two distinct stations: pre-existing Kirkpatrick-Baez (KB) mirror optics...
Increasing evidence suggests that metal dyshomeostasis plays an important role in human neurodegenerative diseases. Although distinctive distributions are described for mature hippocampus and cortex, much less is known about levels intracellular distribution individual hippocampal neuronal somata. To solve this problem, we conducted quantitative analyses utilizing synchrotron radiation X-Ray fluorescence on frozen hydrated primary cultured neurons derived from rat embryonic cortex (CTX) two...
The recent advancement of nanotechnology has provided unprecedented opportunities for the development nanoparticle enabled technologies detecting and treating cancer.Here, we reported construction a PET trackable organic nanoplatform based on phage particle targeted tumor imaging.Method: integrin α v β 3 was constructed by expressing RGD peptides its surface.The target binding affinity this engineered evaluated in vitro.A bifunctional chelator (BFC)...
Abstract Biobanks containing formalin-fixed, paraffin-embedded (FFPE) tissues from animals and human atomic-bomb survivors exposed to radioactive particulates remain a vital resource for understanding the molecular effects of radiation exposure. These samples are often decades old prepared using harsh fixation processes which limit sample imaging options. Optical hematoxylin eosin (H&E) stained may be only feasible processing option, however, H&E images provide no information about...
Rapidly-frozen hydrated (cryopreserved) specimens combined with cryo-scanning x-ray fluorescence microscopy provide an ideal approach for investigating elemental distributions in biological cells and tissues. However, because cryopreservation does not deactivate potentially infectious agents associated Risk Group 2 materials, one must be concerned contamination of expensive complicated cryogenic microscopes when working such materials. We employed ultraviolet germicidal irradiation to...
We describe and demonstrate an optimization-based X-ray image reconstruction framework called Adorym. Our provides a generic forward model, allowing one code to be used for wide range of imaging methods ranging from near-field holography fly-scan ptychographic tomography. By using automatic differentiation optimization, Adorym has the flexibility refine experimental parameters including probe positions, multiple hologram alignment, object tilts. It is written with strong support parallel...
Hypersensitive reaction and pathogenicity (hrp) genes are required for Pseudomonas syringae pv. tomato (Pst) DC3000 to cause disease in susceptible Arabidopsis thaliana plants elicit the hypersensitive response resistant plants. The hrp encode a type III protein secretion system known as Hrp system, which Pst secretes HrpA, HrpZ, HrpW, AvrPto assembles surface appendage, named pilus, hrp-gene-inducing minimal medium. HrpA has been suggested be pilus structural on basis of copurification...
The pluripotency of human embryonic stem cells (hESCs) is important to investigations early development and cell replacement therapy, but the mechanism behind incompletely understood. Zinc has been shown play a key role in differentiation non-pluripotent types, here its hESCs directly examined. By mapping distribution metals at high resolution by x-ray fluorescence microprobe (XFM) analyzing subcellular metal content, we have found evidence that loss correlated with an increase nuclear zinc....
Trace elements, particularly metals, play an important role in a large variety of cellular processes biological system. In the context organisms and tissues, term trace element means that over entire organism is present at only levels, 100 ppm or lower. distribution content can be analyzed using several techniques, for example, visible light optical fluorescence imaging, energy-dispersive X-ray spectroscopy on electron microscope, synchrotron-based (XRF) secondary-ion mass spectrometry,...
Unique functional materials provide a platform as scaffolds for cell/tissue regeneration. Investigation of cell-materials' chemical and biological interactions will enable the application more in area bioengineering, which provides pathway to novel treatment patients who suffer from tissue/organ damage face limitation donation sources. Many studies have been made into Development new substrate platforms regeneration is key research area. Studies discussed this paper focus on investigation...