A5100366167- Advanced X-ray Imaging Techniques
- Advanced Photocatalysis Techniques
- Advanced Electron Microscopy Techniques and Applications
- X-ray Spectroscopy and Fluorescence Analysis
- Algal biology and biofuel production
- Trace Elements in Health
- RNA modifications and cancer
- TiO2 Photocatalysis and Solar Cells
- Electrocatalysts for Energy Conversion
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Advanced X-ray and CT Imaging
- Medical Imaging Techniques and Applications
- Solar-Powered Water Purification Methods
- Photosynthetic Processes and Mechanisms
- Gas Sensing Nanomaterials and Sensors
- Electron and X-Ray Spectroscopy Techniques
- Heavy Metal Exposure and Toxicity
- Aluminum Alloys Composites Properties
- Advanced biosensing and bioanalysis techniques
- Metalloenzymes and iron-sulfur proteins
- CO2 Reduction Techniques and Catalysts
- Metal Extraction and Bioleaching
- Laser-Plasma Interactions and Diagnostics
- Advanced Sensor and Energy Harvesting Materials
Ningbo Product Quality Supervision and Inspection Institute
2022-2025
Shanghai Sixth People's Hospital
2024
Argonne National Laboratory
2015-2024
Chinese University of Hong Kong
2023-2024
Shantou University
2023-2024
Wuhan Textile University
2022-2024
Shantou University Medical College
2024
Huzhou University
2021-2024
Open Source Science Project
2024
Guangzhou Medical University
2024
RNA interference–based spherical nucleic acid nanoconjugates are a potentially safe and brain-penetrant approach for glioblastoma treatment.
We report direct evidence of the rapid self-decomposition graphitic carbon nitride (g-C3N4), a popular photo (electro) catalyst, during gas–solid photocatalytic reaction. Crucially, average rate CO production from light-induced g-C3N4 in Ar is almost equal to that CO2 atmosphere, and products include CO, CO2, NO2, NO2–/NO3–. Using experimental theoretical studies, we reveal chemical instability related adsorbed hydroxyl groups (OHads) on catalyst surface. Specifically, electronic...
Solar-powered interfacial evaporation has been recognized to be a promising and sustainable technology for seawater desalination, in view of the challenge freshwater scarcity fossil energy storage. Nevertheless, current cutting-edge systems mostly ignore issues intermittent solar irradiation bacterial contamination. We have hereby developed novel type an evaporator equipped with carbon black (CB)/chitosan (CS)-decorated phase-change microcapsules as multifunctional photothermal material...
Recent studies have uncovered the therapeutic potential of elesclomol (ES), a copper-ionophore, for copper deficiency disorders. However, we currently do not understand mechanism by which brought into cells as ES–Cu(II) is released and delivered to cuproenzymes present in different subcellular compartments. Here, utilized combination genetic, biochemical, cell-biological approaches demonstrate that intracellular release from ES occurs inside outside mitochondria. The mitochondrial matrix...
Significance X-ray fluorescence microscopy provides unparalleled sensitivity for measuring the distribution of trace elements in many-micrometer-thick specimens, whereas ptychography offers a path to imaging weakly fluorescing biological ultrastructure at beyond-focusing-optic resolution. We demonstrate here first time, our knowledge, combination and frozen-hydrated specimens cryogenic temperatures, with excellent structural chemical preservation. This combined approach will have significant...
In this paper, an intracellular glutathione (GSH) responsive mesoporous silica nanoparticle (MSN-S-S-RGD) was developed as a drug nanocarrier by immobilizing the gatekeeper (RGD containing peptide) onto MSNs using disulfide bonds. The antitumor drug, DOX loaded porous structure of and DOX@MSN-S-S-RGD system has been proved to be effective nanocarrier. It determined that most could entrapped with only slight leakage. After being accumulated in tumor cells via receptor-mediated endocytosis,...
Sequestration within the cytoplasm often limits efficacy of therapeutic nanoparticles that have specific subcellular targets. To allow for both cellular and nanoparticle delivery, we created epidermal growth factor receptor (EGFR)-targeted Fe3O4@TiO2 use native intracellular trafficking EGFR to improve internalization nuclear translocation in EGFR-expressing HeLa cells. While bound EGFR, these do not interfere with interaction between karyopherin-β, a protein is critical ligand-bound...
X-ray microscopy can be used to image whole, unsectioned cells in their native hydrated state. It complements the higher resolution of electron for submicrometer thick specimens, and molecule-specific imaging capabilites fluorescence light microscopy. We describe here first use fast, continuous x-ray scanning frozen simultaneous sub-20 nm ptychographic transmission with high contrast, sub-100 deconvolved diffusible bound ions at concentrations, without need add specific labels. By working...
Accurate knowledge of elemental distributions within biological organisms is critical for understanding their cellular roles. The ability to couple this with overall architecture in three dimensions (3D) deepens our chemistry. Using a whole, frozen-hydrated Chlamydomonas reinhardtii cell as an example, we report the development 3D correlative microscopy through combination simultaneous cryogenic x-ray ptychography and fluorescence microscopy. By taking advantage recently developed...
Summary Trace metals play important roles in biological function, and x‐ray fluorescence microscopy (XFM) provides a way to quantitatively image their distribution within cells. The faithfulness of these measurements is dependent on proper sample preparation. Using mouse embryonic fibroblast NIH/3T3 cells as an example, we compare various approaches the preparation adherent mammalian for XFM imaging under ambient temperature. Direct side‐by‐side comparison shows that plunge‐freezing‐based...
Abstract As x-ray and electron tomography is pushed further into the nanoscale, limitations of rotation stages become more apparent, leading to challenges in alignment acquired projection images. Here we present an approach for rapid post-acquisition these projections obtain high quality three-dimensional Our based on a joint estimation errors, object, using iterative refinement procedure. With simulated data where know error each image, our shows residual that factor thousand smaller, it...
Motivated by the advanced photon source upgrade, a new hard X-ray microscope called "Velociprobe" has been recently designed and built for fast ptychographic imaging with high spatial resolution. We are addressing challenges of high-resolution scanning novel hardware designs, motion controls, data acquisition strategies, including use high-bandwidth interferometric measurements. The granite, air-bearing-supported stages provides necessary long travel ranges coarse to accommodate real samples...
Semiconductor-based biointerfaces are typically established either on the surface of plasma membrane or within cytoplasm. In Gram-negative bacteria, periplasmic space, characterized by its confinement and presence numerous enzymes peptidoglycans, offers additional opportunities for biomineralization, allowing nongenetic modulation interfaces. We demonstrate semiconductor nanocluster precipitation containing single- multiple-metal elements periplasm, as observed through various electron-...
The glymphatic system is a perivascular fluid transport for waste clearance. Glymphatic believed to be driven by the pumping effect created pulsation of arterial wall caused cardiac cycle. Ultrasound sonication circulating microbubbles (MBs) in cerebral vasculature induces volumetric expansion and contraction MBs that push pull on vessel generate MB effect. objective this study was evaluate whether can mechanically manipulated focused ultrasound (FUS) MBs. pathway intact mouse brains studied...