A5039034888- Effects of Radiation Exposure
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Radiation Therapy and Dosimetry
- X-ray Spectroscopy and Fluorescence Analysis
- Radiation Dose and Imaging
- Advanced X-ray Imaging Techniques
- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- DNA Repair Mechanisms
- Advanced MRI Techniques and Applications
- Neuroblastoma Research and Treatments
- RNA and protein synthesis mechanisms
- Hepatocellular Carcinoma Treatment and Prognosis
- Trace Elements in Health
- Medical Imaging Techniques and Applications
- MRI in cancer diagnosis
- Radiopharmaceutical Chemistry and Applications
- Lanthanide and Transition Metal Complexes
- Molecular Biology Techniques and Applications
- Cancer-related Molecular Pathways
- Carcinogens and Genotoxicity Assessment
- Electron and X-Ray Spectroscopy Techniques
- DNA and Nucleic Acid Chemistry
- Nanoparticles: synthesis and applications
Northwestern University
2016-2025
Midwestern University
2018-2024
Robert H. Lurie Comprehensive Cancer Center of Northwestern University
2006-2023
Argonne National Laboratory
1996-2020
Stanford Synchrotron Radiation Lightsource
2020
University of Wisconsin–Milwaukee
2020
Northwestern Memorial Hospital
2005-2019
Radiation Oncology Associates
2019
Northwest University
2016
Institute of Biophysics
2012
While few publications have documented the uptake of nanoparticles in plants, this is first study describing and distribution ultrasmall anatase TiO2 plant model system Arabidopsis. We modified nanoparticle surface with Alizarin red S sucrose demonstrated that nanoconjugates traversed cell walls, entered into cells, accumulated specific subcellular locations. Optical X-ray fluorescence microscopy coregistered vacuoles nuclei.
RNA interference–based spherical nucleic acid nanoconjugates are a potentially safe and brain-penetrant approach for glioblastoma treatment.
The concept of sequencing by hybridization (SBH) makes use an array all possible n-nucleotide oligomers (n-mers) to identify n-mers present in unknown DNA sequence. Computational approaches can then be used assemble the complete As a validation this concept, sequences three fragments, 343 base pairs length, were determined with octamer oligonucleotides. Possible applications SBH include physical mapping (ordering) overlapping clones, sequence checking, fingerprinting comparisons normal and...
Abstract In the following review we discuss several types of nanoparticles (such as TiO 2 , quantum dots, and gold nanoparticles) their impact on ability to image biological components in fixed cells. The also discusses factors influencing nanoparticle imaging uptake live cells vitro. Due unique size-dependent properties offer numerous advantages over traditional dyes proteins. For example, photostability, narrow emission peak, rationally modify both size surface chemistry Quantum Dots allow...
Hard X-ray fluorescence microscopy is one of the most sensitive techniques for performing trace elemental analysis biological samples such as whole cells and tissues. Conventional sample preparation methods usually involve dehydration, which removes cellular water may consequently cause structural collapse, or invasive processes embedding. Radiation-induced artifacts also become an issue, particularly spatial resolution increases beyond sub-micrometer scale. To allow imaging under hydrated...
Sequestration within the cytoplasm often limits efficacy of therapeutic nanoparticles that have specific subcellular targets. To allow for both cellular and nanoparticle delivery, we created epidermal growth factor receptor (EGFR)-targeted Fe3O4@TiO2 use native intracellular trafficking EGFR to improve internalization nuclear translocation in EGFR-expressing HeLa cells. While bound EGFR, these do not interfere with interaction between karyopherin-β, a protein is critical ligand-bound...
Summary Trace metals play important roles in biological function, and x‐ray fluorescence microscopy (XFM) provides a way to quantitatively image their distribution within cells. The faithfulness of these measurements is dependent on proper sample preparation. Using mouse embryonic fibroblast NIH/3T3 cells as an example, we compare various approaches the preparation adherent mammalian for XFM imaging under ambient temperature. Direct side‐by‐side comparison shows that plunge‐freezing‐based...
Deoxyribonucleic acid (DNA) oligonucleotides hybridize to matching DNA sequences in cells, as established the literature, depending on active transcription of target sequence and local molarity oligonucleotide. We investigated intracellular distribution nanoconjugates composed attached TiO2 nanoparticles, thus creating a locally increased concentration Two types nanoconjugates, with mitochondrial or nucleolar DNA, were specifically retained mitochondria nucleoli.
Abstract Resistance to anthracyclines and other chemotherapeutics due P-glycoprotein (pgp)-mediated export is a frequent problem in cancer treatment. Here, we report that iron oxide–titanium dioxide core-shell nanocomposites can serve as efficient carriers for doxorubicin overcome this common mechanism of drug resistance cells. Doxorubicin nanocarriers (DNC) increased effective uptake drug-resistant ovarian Mechanistically, bound the TiO2 surface by labile bond was severed upon acidification...
Recent efforts have shown that nanoscale materials, specifically, metal-based nanoparticles, hold particular promise for the development of multifunctional imaging probes. These new materials provide means to chaperone and concentrate both drugs contrast agents in specific organs, tissues, cells. Therefore, we prepared a Gd(III)-modified DNA−TiO2 semiconducting nanoparticle is detectable cells by MR imaging. labeled particles are retained at subcellular locations via DNA hybridization...
Abstract Visualization of nanoparticles without intrinsic optical fluorescence properties is a significant problem when performing intracellular studies. Such the case with titanium dioxide (TiO 2 ) nanoparticles. These nanoparticles, electronically linked to single‐stranded DNA oligonucleotides, have been proposed be used both as gene knockout devices and possible tumor imaging agents. By interacting complementary target sequences in living cells, these photoinducible TiO –DNA...
To combine dynamic contrast material-enhanced (DCE) magnetic resonance (MR) imaging with x-ray fluorescence microscopy (XFM) of mammary gland tissue samples from mice to identify the spatial distribution gadolinium after intravenous injection.C3(1) Sv-40 large T antigen transgenic (n = 23) were studied institutional animal care and use committee approval. Twelve underwent DCE MR injection gadodiamide, concentration-time curves fit a two-compartment pharmacokinetic model following parameters:...
Summary Surface functionalization of nanoparticles has become an important tool for in vivo delivery bioactive agents to their target sites. Here we describe the reverse strategy, nanoharvesting, which are used as a isolate compounds from living cells. Anatase TiO 2 smaller than 20 nm form strong bonds with molecules bearing enediol and especially catechol groups. We show that these enter plant cells, conjugate group‐rich flavonoids situ , exit cells flavonoid‐nanoparticle conjugates. The...
Abstract Saliva, as a non-invasive and easily accessible biofluid, has been shown to contain RNA biomarkers for prediction diagnosis of several diseases. However, systematic analysis done by our group identified two problematic issues not coherently described before: (1) most the isolated originates from oral microbiome (2) amount human is comparatively low. The degree bacterial contamination showed ratios up 1:900,000, so that only about one out 900,000 copies was origin, but quality...
Determination of the sequences human and other complex genomes requires much faster less expensive sequencing processes than methods in use today. Sequencing by hybridization is potentially such a process. In this paper we present data sufficient to accurately read known sequence 100 base pairs. independent reactions, octamer nonamer oligonucleotides derived from hybridized more strongly DNA controls. The 93 consecutive overlapping probes were 100-base-pair segment test additional generated...