A5040115265- Plant Pathogenic Bacteria Studies
- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Plant pathogens and resistance mechanisms
- Plant Parasitism and Resistance
- Plant Pathogens and Fungal Diseases
- Polysaccharides and Plant Cell Walls
- Enzyme Production and Characterization
- Bacterial Genetics and Biotechnology
- Plant Virus Research Studies
- Genomics and Phylogenetic Studies
- Plant Disease Resistance and Genetics
- Banana Cultivation and Research
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Mycorrhizal Fungi and Plant Interactions
- Yersinia bacterium, plague, ectoparasites research
- GABA and Rice Research
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Microbial Metabolism and Applications
- Toxin Mechanisms and Immunotoxins
- Enzyme Structure and Function
- Enzyme Catalysis and Immobilization
- Biofuel production and bioconversion
Cornell University
2012-2023
Plant (United States)
2001-2023
King Abdulaziz University
2013
GTx (United States)
1997-2010
United States Department of Agriculture
2000-2009
Agricultural Research Service
2002-2009
Michigan State University
1996-2003
University of Nebraska–Lincoln
2002-2003
University of Basilicata
2003
University of Wisconsin–Madison
1999-2002
cell components. A convincing role in pathogenesis, however, has been es tablished only for those enzymes that attack the pectic fraction of plant wall. Several fundamental advances our understanding these have made since tissue degradation was reviewed this series by Bateman & Millar (17) twenty years ago. In past decade it demonstrated highly purified could macer ate and kill tissues a manner similar to occurring soft-rot diseases; same be used disassemble dicot primary wall, thus...
We report the complete genome sequence of model bacterial pathogen Pseudomonas syringae pathovar tomato DC3000 (DC3000), which is pathogenic on and Arabidopsis thaliana . The (6.5 megabases) contains a circular chromosome two plasmids, collectively encode 5,763 ORFs. identified 298 established putative virulence genes, including several clusters genes encoding 31 confirmed 19 predicted type III secretion system effector proteins. Many were members paralogous families also proximal to mobile...
A proteinaceous elicitor of the plant defense reaction known as hypersensitive response was isolated from Erwinia amylovora , bacterium that causes fire blight pear, apple, and other rosaceous plants. The elicitor, named harpin, is an acidic, heat-stable, cell-envelope-associated protein with apparent molecular weight 44 kilodaltons. Harpin caused tobacco leaf lamina to collapse increase in pH bathing solutions suspension-cultured cells. gene encoding harpin ( hrpN ) located 40-kilobase hrp...
The gamma-proteobacterial plant pathogen Pseudomonas syringae pv. tomato DC3000 uses the type III secretion system to inject ca. 28 Avr/Hop effector proteins into plants, which enables bacterium grow from low inoculum levels produce bacterial speck symptoms in tomato, Arabidopsis thaliana, and (when lacking hopQ1-1) Nicotiana benthamiana. effectors are collectively essential but individually dispensable for ability of bacteria defeat defenses, grow, plants. Eighteen genes clustered six...
The plant pathogenic bacterium Pseudomonas syringae is divided into pathovars differing in host specificity, with P. pv. ( Psy ) and tomato Pto representing particularly divergent pathovars. hrp / hrc genes encode a type III protein secretion system that appears to translocate Avr Hop effector proteins cells. DNA sequence analysis of the regions 61, B728a, DC3000 has revealed Hrp pathogenicity island (Pai) tripartite mosaic structure. gene cluster conserved all three strains flanked by...
of transposon mutagenesis, broad-host-range cosmid vectors, and marker-exchange mutagenesis to identify manipulate bacterial genes that have a readily scored phenotype when mutated, conjugated into related strain, or expressed in Escherichia coli. These approaches yielded large inventory hrp (hypersensitive [esponse pathogenicity) avr (eirulence) directly relate the HR puzzle as we!l numerous other associated with pectic enzyme, toxin, extracellular polysaccharide (EPS) production. Rather...
Pseudomonas syringae pv. phaseolicola, a gram-negative bacterial plant pathogen, is the causal agent of halo blight bean. In this study, we report on genome sequence P. phaseolicola isolate 1448A, which encodes 5,353 open reading frames (ORFs) one circular chromosome (5,928,787 bp) and two plasmids (131,950 bp 51,711 bp). Comparative analyses with phylogenetically divergent pathovar, tomato DC3000, revealed strong degree conservation at gene levels. total, 4,133 ORFs were identified as...
The ability of Pseudomonas syringae pv. tomato DC3000 to parasitize and Arabidopsis thaliana depends on genes activated by the HrpL alternative sigma factor. To support various functional genomic analyses DC3000, specifically, identify involved in pathogenesis, we developed a draft sequence used an iterative process involving computational gene expression techniques virulence-implicated downstream HrpL-responsive promoters. Hypersensitive response pathogenicity (Hrp) promoters are known...
The Pseudomonas syringae pv. tomato DC3000 type III secretion system (TTSS) is required for bacterial pathogenicity on plants and elicitation of the hypersensitive response (HR), a programmed cell death (PCD) that occurs resistant plants. Cosmid pHIR11 enables non-pathogens to elicit an HR dependent upon TTSS effector HopPsyA. We used determine effectors HopPtoE, avirulence AvrPphEPto, AvrPpiB1Pto, AvrPtoB, HopPtoF could suppress HopPsyA-dependent tobacco Arabidopsis. Mixed inoculum...
The ability of Pseudomonas syringae pv. tomato DC3000 to be pathogenic on plants depends the Hrp (hypersensitive response and pathogenicity) type III protein secretion system effector proteins it translocates into plant cells. Through iterative application experimental computational techniques, inventory has been substantially enlarged. Five homologs known avirulence (Avr) five candidates, encoded by genes with putative promoters signatures horizontal acquisition, were demonstrated secreted...
Summary The model pathogen Pseudomonas syringae pv. tomato DC3000 causes bacterial speck in and Arabidopsis, but Nicotiana benthamiana , an important plant, is considered to be a non‐host. Strain injects approximately 28 effector proteins into plant cells via the type III secretion system (T3SS). These were individually delivered N. leaf T3SS‐proficient fluorescens eight, including HopQ1‐1, showed some capacity cause cell death this test. Four gene clusters encoding 13 effectors deleted from...
The nonpathogenic bacteria Pseudomonas fluorescens and Escherichia coli can elicit a genotype-specific hypersensitive response (HR) in plants if they express both the HR pathogenesis (Hrp) protein secretion system HrpZ harpin from P. syringae pv 61 avirulence (avr) gene whose presence is recognized by corresponding disease resistance plant. We have found that recognition event appears to require transfer of Avr into plant cell. Elicitation was observed with avrB+ soybean Arabidopsis carrying...
A cosmid clone isolated from a genomic library of Pseudomonas syringae pv. 61 restored to all Tn5 mutants this strain studied the ability elicit hypersensitive response (HR) in tobacco. Cosmid pHIR11 also enabled Escherichia coli TB1 an HR-like reaction when high levels inoculum (10(9) cells per ml) were infiltrated into tobacco leaves. The cosmid, which contains 31-kilobase DNA insert, was mobilized by triparental matings fluorescens 55 (a nonpathogen that normally causes no plant...
The virulence of Pseudomonas syringae and many other proteobacterial pathogens is dependent on complex repertoires effector proteins injected into host cells by type III secretion systems. 28 well-expressed genes in the repertoire model pathogen P. pv. tomato DC3000 were deleted to produce polymutant DC3000D28E. Growth DC3000D28E Nicotiana benthamiana was symptomless 4 logs lower than that DC3000Δ hopQ1-1 , which causes disease this plant. seemed functionally effectorless but otherwise WT...