A5030391002- Advances in Cucurbitaceae Research
- Cocoa and Sweet Potato Agronomy
- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Virus Research Studies
- Plant Gene Expression Analysis
- Photosynthetic Processes and Mechanisms
- Plant Pathogenic Bacteria Studies
- Plant biochemistry and biosynthesis
- Plant Disease Resistance and Genetics
- Plant Physiology and Cultivation Studies
- Powdery Mildew Fungal Diseases
- Phytoplasmas and Hemiptera pathogens
- Chromosomal and Genetic Variations
- Polysaccharides and Plant Cell Walls
- Plant Disease Management Techniques
- Plant Pathogens and Fungal Diseases
- Light effects on plants
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Food Quality and Safety Studies
- Plant Pathogens and Resistance
- Postharvest Quality and Shelf Life Management
- Phytochemicals and Antioxidant Activities
- Phytochemistry and Biological Activities
Shanghai Jiao Tong University
2015-2024
University of Wisconsin–Madison
2016-2018
Shenyang Agricultural University
2011
Shandong Agricultural University
2009
Powdery mildew is a common disease in plants, and it also one of the main diseases middle final stages cucumber [Cucumis sativus]. on plant leaves affects photosynthesis, which may reduce yield. Therefore, great significance to automatically identify powdery mildew. Currently, most image-based models commonly regard identification problem as dichotomy case, yielding true or false classification assertion. However, quantitative assessment resistance traits plays an important role screening...
Sex determination in plants involves a variety of mechanisms. Here, we report the map-based cloning and characterization unisexual-flower-controlling gene M. M was identified as previously characterized putative 1-aminocyclopropane-1-carboxylic acid synthase gene, while m allele that mutated at conserved site (Gly33Cys) lost activity original enzymatically active allele.
Structural variations (SVs) represent a major source of genetic diversity. However, the functional impact and formation mechanisms SVs in plant genomes remain largely unexplored. Here, we report nucleotide-resolution SV map cucumber (Cucumis sativas) that comprises 26,788 based on deep resequencing 115 diverse accessions. The largest proportion was formed through nonhomologous end-joining rearrangements, occurrence is closely associated with regions high nucleotide These affect coding 1676...
Powdery mildew (PM) is a serious fungal disease of cucumber worldwide. The identification resistance genes very important for breeding to ensure production. Here, natural loss-of-function mutations at an MLO homologous locus, CsMLO1, were found confer durable PM in cucumber. CsMLO1 encoded cell membrane protein, was mainly expressed leaves and cotyledons, up-regulated by the early stage host-pathogen interaction. Ectopic expression rescued phenotype resistant Atmlo2 Atmlo12 double mutant...
The Gy14 cucumber (Cucumis sativus) is resistant to oomyceteous downy mildew (DM), bacterial angular leaf spot (ALS) and fungal anthracnose (AR) pathogens, but the underlying molecular mechanisms are unknown. Quantitative trait locus (QTL) mapping for disease resistances in further map-based cloning identified a candidate gene loci, which was validated functionally characterized by spatial-temporal expression profiling, allelic diversity phylogenetic analysis, as well local association...
Summary Cucumber fruits that have tubercules and spines (trichomes) are known to possess a warty (Wty) phenotype. In this study, the tuberculate fruit gene Tu was identified by map‐based cloning, found encode transcription factor (TF) with single C 2 H zinc finger domain. in all 38 Wty lines examined, completely absent from 56 non‐warty ( nW ty) lines. plants transgenic for (TCP) revealed required Subcellular localization showed fusion protein GFP–Tu localized mainly nucleus. Based on...
It is well established that the plant hormone ethylene plays a key role in cucumber sex determination. Since unisexual control gene M was cloned and shown to encode an synthase, instead of receptor, 'one-hormone hypothesis', which used explain phenotype, has been challenged. Here, physiological function CsACS2 (the encoded by locus) studied using transgenic tobacco system. The results indicated overexpression increased production plant, native promoter had no activity (PM). However, when PM...
High-density genetic linkage map plays an important role in genome assembly and quantitative trait loci (QTL) fine mapping. Since the coming of next-generation sequencing, makes structure high-density maps much more convenient practical, which simplifies SNP discovery high-throughput genotyping. In this research, a cucumber was structured using specific length amplified fragment 153 F2 populations S1000 × S1002. The composed 3,057 SLAFs, including 4,475 markers on seven chromosomes, spanned...
Abstract Plant trichomes serve as a highly suitable model for investigating cell differentiation at the single‐cell level. The regulatory genes involved in unicellular trichome development Arabidopsis thaliana have been intensively studied, but regulating multicellular plants remain unclear. Here, we characterized Cucumis sativus (cucumber) representative and unbranched structures, identified Micro‐trichome ( Mict ), using map‐based cloning an F 2 segregating population of 7,936 individuals...
The leaf is an important photosynthetic organ and plays essential role in the growth development of plants. Leaf color mutants are ideal materials for studying chlorophyll metabolism, chloroplast development, photosynthesis. In this study, we identified EMS-induced mutant, yl2.1, which exhibited yellow cotyledons true leaves that did not turn green with growth. yl2.1 locus was controlled by a recessive nuclear gene. CsYL2.1 mapped to 166.7-kb genomic region on chromosome 2, contains 24...
Abstract A 173‐point genetic linkage map of cucumber ( Cucumis sativus L.), consisting 116 SRAPs, 33 RAPDs, 11 SSRs, 9 SCARs, 3 ISSRs, and 1 STS, was constructed using 130 F 2 progeny derived from a narrow cross between line S94 (Northern China open‐field type) S06 (greenhouse European type). The seven groups spanned 1016 cM with mean marker interval 5.9 cM. Using the population its families, total 38 QTLs were detected on five an LOD threshold 3.0 for nine fruit‐related traits: fruit...
In cucumber (Cucumis sativus L.), the differentiation and development of female flowers are important processes that directly affect fruit yield quality. Sex is mainly controlled by three ethylene synthase genes, F (CsACS1G), M (CsACS2) A (CsACS11). Thus, plays a key role in sex differentiation. The "one-hormone hypothesis" posits regulate levels initiate flower cucumber. Nonetheless, precise molecular mechanism this process remains elusive. To investigate which phenotype, near-isogenic...
A lot of researches have been focused on the evolution and function MYB transcription factors (TFs). For revealing formation petunia flower color diversity, gene family in was identified analyzed. In this study, a total 155 genes, including 40 1R-MYBs, 106 R2R3-MYBs, 7 R1R2R3-MYBs 2 4R-MYBs, Petunia axillaris genome. Most R2R3 genes contain three exons two introns, whereas number PaMYB introns varies from 0 to 12. The R2R3-MYB members could be divided into 28 subgroups. Analysis structure...
TCP proteins are plant-specific transcription factors widely implicated in leaf morphogenesis and senescence, flowering, lateral branching, hormone crosstalk, stress responses. However, the relationship between pattern of TCPs organ development cucumber has not been systematically studied. In this study, we performed a genome-wide identification putative genes analyzed their chromosomal location, gene structure, conserved motif, transcript expression. A total 27 were identified characterized...
Abstract Trichomes and fruit spines are important traits that directly affect the appearance quality commercial value of cucumber (Cucumis sativus). Tril (Trichome-less), encodes a HD-Zip IV transcription factor plays crucial role in initiation trichomes spines, but little is known about details regulatory mechanisms involved. In this study, analysis tissue expression patterns indicated expressed functions early stages organ development. Expression under control its own promoter (the...
Abstract Powdery mildew (PM) is an economically important disease of cucumber ( Cucumis sativus L . ) caused by the obligate biotrophic pathogen, Podosphaera xanthii, in greenhouse and field. In this study, a resistant breeding line S06 (greenhouse European type) susceptible inbred S94 (Northern China open‐field their F 2:3 populations were used to investigate PM resistance under seedling spray inoculation two growing seasons leaf disc infection one season. Quantitative trait loci (QTL)...