A5049651079- RNA and protein synthesis mechanisms
- RNA modifications and cancer
- Bacterial Genetics and Biotechnology
- Plant biochemistry and biosynthesis
- Antibiotic Resistance in Bacteria
- Cancer therapeutics and mechanisms
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- Phytochemical compounds biological activities
- Metabolism, Diabetes, and Cancer
- Plant Gene Expression Analysis
- RNA Interference and Gene Delivery
- X-ray Diffraction in Crystallography
- Neurobiology and Insect Physiology Research
- Cancer Genomics and Diagnostics
- Synthesis and biological activity
- Crystallization and Solubility Studies
- MicroRNA in disease regulation
- CRISPR and Genetic Engineering
- Cancer, Hypoxia, and Metabolism
- Environmental Policies and Emissions
- Sulfur Compounds in Biology
- Management Theory and Practice
- Circular RNAs in diseases
- Human Behavior and Motivation
Shanghai East Hospital
2021-2024
Shanghai Jiao Tong University
2021-2024
Heilongjiang University of Chinese Medicine
2024
Shanghai Medical College of Fudan University
2013-2024
Fudan University Shanghai Cancer Center
2022-2024
Kunming Children's Hospital
2024
Kunming Medical University
2024
Fudan University
2011-2020
Pudong Medical Center
2019-2020
Chinese Academy of Agricultural Sciences
2010-2014
Schizophrenia is a disease with complex pathological mechanism that influenced by multiple genes. The study of its pathogenesis dominated the dopamine hypothesis, as well other hypotheses such 5-hydroxytryptamine glutamate immune-inflammatory gene expression abnormality and neurodevelopmental hypothesis. first generation antipsychotics was developed based on dopaminergic receptor antagonism, which blocks D2 receptors in brain to exert antipsychotic effects. second acts dual blockade...
The sesquiterpene lactone artemisinin is an important anti-malarial component produced by the glandular secretory trichomes of sweet wormwood (Artemisia annua L.). Light was previously shown to promote production, but underlying regulatory mechanism remains elusive. In this study, we demonstrate that ELONGATED HYPOCOTYL 5 (HY5), a central transcription factor in light signaling pathway, cannot biosynthesis on its own, as binding AaHY5 promoters biosynthetic genes failed activate their...
A lot of researches have been focused on the evolution and function MYB transcription factors (TFs). For revealing formation petunia flower color diversity, gene family in was identified analyzed. In this study, a total 155 genes, including 40 1R-MYBs, 106 R2R3-MYBs, 7 R1R2R3-MYBs 2 4R-MYBs, Petunia axillaris genome. Most R2R3 genes contain three exons two introns, whereas number PaMYB introns varies from 0 to 12. The R2R3-MYB members could be divided into 28 subgroups. Analysis structure...
Cell surface RNAs, notably glycoRNAs, have been reported, yet the exact RNA compositions in different cell types remain unclear. Here, we introduce a comprehensive suite of methodologies for imaging, profiling, quantifying, and exploring biological functions specific RNAs. Utilizing these techniques, identified diverse non-coding RNAs present on mammalian surfaces. We confirm membrane anchorage quantify abundance several representative human primary cells. Notably, discover significant...
Abstract BACKGROUND: In recent years, concerns over the potential impact of synthetic pesticides on environment have made discovery and development environmentally friendly a more pressing issue. Camptothecin (CPT) its derivatives attracted much attention owing to their unique mechanisms action against insects. this paper, two insect cell lines, Sf21 IOZCAS‐Spex‐II, were used conduct preliminary investigation CPT hydroxycamptothecin (HCPT) as inducers apoptosis in illustrate mechanism...
The plant Artemisia annua L. is famous for producing "artemisinin", which an essential component in the treatment of malaria. glandular secretory trichomes (GSTs) on leaves A. secrete and store artemisinin. Previous research has demonstrated that raising GST density can effectively raise artemisinin content. However, molecular mechanism initiation not fully understood yet. In this study, we identified MYB transcription factor, AaMYB108-like, co-induced by light jasmonic acid, positively...
Ribosome stalling on ermBL at the tenth codon (Asp) is believed to be a major mechanism of ermB induction by erythromycin (Ery). In this study, we demonstrated that Ery depends not only expression but also previously unreported ermBL2 expression. Introducing premature termination codons in , proved translation N-terminal region key component for induced Ery, whereas C-terminal did affect Ery-induced . Mutation (Asp10) with other amino acids showed degree vivo was completely consistent data...
The acquisition of antibiotic resistance by human pathogens poses a significant threat to public health. mechanisms that control the proliferation and expression genes are not yet completely understood. aminoglycosides historically important class antibiotics were introduced in 1940s. Aminoglycoside is conferred most commonly through enzymatic modification drug or target rRNA methylation overexpression efflux pumps. In our recent paper, we reported aminoglycoside encoding acetyl transferase...
Camptothecin (CPT), a plant alkaloid originally isolated from the native Chinese tree, Camptotheca acuminate, exerts toxic effect by targeting eukaryotic DNA topoisomerase 1 (DNA Topo1). Besides as potent anti-cancer agents, CPT and its derivatives are now being explored potential pesticides for insect control. In this study, we assessed their toxicity to an homolog, Topo1 protein beet armyworms (Spodoptera exigua Hübner), worldwide pest of many important crops. The S. gene contains ORF 2790...
Hepatocellular carcinoma (HCC) is a highly heterogeneous cancer with major challenges in both prevention and therapy. Metformin, adenosine monophosphate-activated protein kinase (AMPK) activator, has been suggested to reduce the incidence of HCC when used for patients diabetes preclinical clinical studies. However, possible effects metformin their mechanisms action non-diabetic have not adequately investigated.
Ascorbic acid (ASC) has been reported to stimulate DNA iterative oxidase ten-eleven translocation (TET) enzymes, Jumonji C-domain-containing histone demethylases, and potentially RNA m6A demethylases FTO ALKBH5 as a cofactor. Although ascorbic widely investigated in reprogramming methylation status vitro, cultured cells mouse models, its specific role the catalytic cycle of dioxygenases remains enigmatic. Here, we systematically stimulation ASC toward TET2, ALKBH3, FTO. We find that...
Class 1 integrons accumulate antibiotic resistance genes by site-specific recombination at aatI-1 sites. Captured are transcribed from a promoter located within the integron; for class integrons, first gene to be and translated normally encodes an aminoglycoside protein (either acetyltransferase [AAC] or adenyltransferase [AAD]). The leader RNA Pseudomonas fluorescens integron contains aminoglycoside-sensing riboswitch that controls expression of downstream gene. Here, we explore...
The proliferation of antibiotic resistance has its origins in horizontal gene transfer. class 1 integrons mediate transfer by assimilating antibiotic-resistance genes through site-specific recombination. For the first assimilated normally encodes an aminoglycoside protein which is either acetyltransferase (AAC), nucleotidyltransferase - (ANT), or adenyl transferase (AAD). An aminoglycoside-sensing riboswitch RNA leader AAC/AAD that controls expression been previously described. Here we...
Abstract The Agrobacterium-mediated transient expression system has been developed and applied to various plants as an alternative stable transformation. However, its application in tomatoes is still limited due low efficiency. In this study, we describe improved vacuum-infiltration that can be used both tomato fruits leaves. Notably, study the first report of vacuum infiltration attached fruits. feasibility Micro-Tom was confirmed by assays, including multiple fluorescent protein analysis,...
Owing to the limited treatment options for hepatocellular carcinoma (HCC), interventions targeting pre-HCC stages have attracted increasing attention. In stage, hepatic tumor-initiating cells (hTICs) proliferate abnormally and contribute hepatocarcinogenesis. Numerous studies investigated targeted senescence induction as an HCC intervention. However, it remains be clarified whether of hTICs could serve a
Creating high-efficient and environment-friendly pesticides is very important to produce the pollution free agriculture food maintain balance of survival environmental human being. According reports, camptothecin (CPT) its derivatives are now being explored as a class botanical insecticide in due novel mode action. In order improve insecticidal activity CPT, ten (1) 10-hydroxycamptothecin (2) (1a, 1b, 1c, 1d, 1e; 2a, 2b, 2c, 2d, 2e) were designed synthesized via esterification with analogs...
Hydrogen sulfide (H(2)S) is a newly identified member of the small family gasotransmitters that are endogenous gaseous signaling molecules have fundamental role in human biology and disease. Although it relatively recent discovery mechanism H(2)S activity not completely understood, known to be involved number cellular processes; can affect ion channels, transcription factors protein kinases mammals.In this paper, we used fission yeast as model organism study global gene expression profile...
The title compound, C(26)H(34)O(11), known as bruceine A, is a natural quassinoid extracted from the dried fruits of Brucea javanica. Its structure consists five fused rings including an oxygen-containing heterocyclic ring and lactone ring. Two intra-molecular O-H⋯O links help to establish mol-ecular conformation. In crystal, hydrogen bonds connect mol-ecules.
Ribosome stalling on ermBL at the tenth codon (Asp) and mRNA stabilization are believed to be mechanisms by which erythromycin (Ery) induces ermB expression. Expression of is also induced 16-membered ring macrolides (tylosin, josamycin spiramycin), but mechanism underlying this induction unknown.We introduced premature termination codons, alanine-scanning mutagenesis amino acid mutations in ermBL2.In paper, we demonstrated that can induce expression not ermC The truncated mutants ermB-coding...