A5024965407- Plant biochemistry and biosynthesis
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Wheat and Barley Genetics and Pathology
- Plant tissue culture and regeneration
- Plant nutrient uptake and metabolism
- Plant Gene Expression Analysis
- Wireless Communication Security Techniques
- Crop Yield and Soil Fertility
- CRISPR and Genetic Engineering
- Plant Stress Responses and Tolerance
- Viral Infectious Diseases and Gene Expression in Insects
- Cancer therapeutics and mechanisms
- Microbial Natural Products and Biosynthesis
- Chaos-based Image/Signal Encryption
- Neurobiology and Insect Physiology Research
- Pharmacological Effects of Natural Compounds
- Insect-Plant Interactions and Control
- Advanced Steganography and Watermarking Techniques
- Sparse and Compressive Sensing Techniques
- Bioenergy crop production and management
- Transgenic Plants and Applications
- Legume Nitrogen Fixing Symbiosis
- Genetic Mapping and Diversity in Plants and Animals
- Malaria Research and Control
Shanghai Jiao Tong University
2019-2024
Institute of Genetics and Developmental Biology
2014-2024
Center for Agricultural Resources Research
2014-2024
Chinese Academy of Sciences
2007-2024
China Academy of Chinese Medical Sciences
2024
Shanghai Innovative Research Center of Traditional Chinese Medicine
2024
Guizhou University
2022-2024
Zhejiang Chinese Medical University
2023-2024
Sun Yat-sen University
2024
Delft University of Technology
2024
Summary In Arabidopsis, AUXIN RESPONSE FACTOR 3 ( ARF 3) belongs to the auxin response factor ) family that regulates expression of auxin‐responsive genes. is known function in leaf polarity specification and gynoecium patterning. this study, we discovered a previously unknown role for floral meristem FM determinacy through isolation characterization mutant enhanced defects agamous (ag)‐10 , weak ag allele. Central players include WUSCHEL WUS ), gene critical maintenance, AG APETALA 2 AP...
Successful floral meristem (FM) determinacy is critical for subsequent reproductive development and the plant life cycle. Although phytohormones cytokinin auxin interact to coregulate many aspects of development, whether how function in FM remain unclear. Here, we show that Arabidopsis thaliana, homeostasis determinacy. In this developmental context, promotes expression AUXIN RESPONSE FACTOR3 (ARF3) repress activity. ARF3 directly represses ISOPENTENYLTRANSFERASE (IPT) family genes...
Summary Artemisinin, isolated from Artemisia annua , is recommended as the preferred drug to fight malaria. Previous research showed that jasmonate (JA)‐mediated promotion of artemisinin accumulation depended on light. However, mechanism underlying interaction light and JA in regulating still unknown. We identified a WRKY transcription factor, AaWRKY9, using transcriptome analysis. The glandular trichome‐specific AaWRKY9 positively regulates biosynthesis by directly binding promoters AaDBR2...
Summary Plant glandular secretory trichomes (GSTs) produce various specialized metabolites. Increasing GST density represents a strategy to enhance the yield of these chemicals; however, gene regulatory network that controls initiation remains unclear. In previous study Artemisia annua L., we found HD‐ZIP IV transcription factor, AaHD1, promotes by directly regulating AaGSW2 . Here, identified two AaHD1‐interacting factors, namely AaMIXTA‐like 2 (AaMYB16) and AaMYB5. Through generation...
A better understanding of wheat functional genomics can improve targeted breeding for agronomic traits and environmental adaptation. However, the lack gene-indexed mutants low transformation efficiency limit in-depth gene studies genetic manipulation breeding. In this study, we created a library KN9204, popular variety in northern China, with reference genome, transcriptome, epigenome different tissues, using ethyl methyl sulfonate (EMS) mutagenesis. This contains vast developmental...
Abstract Artemisinin, also known as ‘Qinghaosu’, is a chemically sesquiterpene lactone containing an endoperoxide bridge. Due to the high activity kill Plasmodium parasites, artemisinin and its derivatives have continuously served foundation for antimalarial therapies. Natural unique traditional Chinese medicinal plant Artemisia annua L., content in this low. This has motivated synthesis of bioactive compound using yeast, tobacco, Physcomitrium patens systems. However, production these...
Wheat spike development is a coordinated process of cell proliferation and differentiation with distinctive phases architecture changes. However, the dynamic alteration gene expression in this remains enigmatic. Here, we characterized dissected bread wheat into six developmental stages, used genome-wide profiling, to investigate underlying regulatory mechanisms. High correlations between any two given stages indicated that early controlled by small subset genes. Throughout, auxin signaling...
Artemisia annua L. is known for its specific product “artemisinin” which an active ingredient curing malaria. Artemisinin secreted and accumulated in the glandular secretory trichomes (GSTs) on A. leaves. Earlier studies have shown that increasing GST density effective artemisinin content. However, mechanism of initiation not fully understood. To this end, we isolated characterized R2R3-MYB gene, AaMYB17 , expressed specifically GSTs shoot tips. Overexpression increased enhanced content,...
Artemisia annua is a medicinal plant rich in terpenes and flavonoids with useful biological activities such as antioxidant, anticancer, antimalarial activities. The transcriptional regulation of flavonoid biosynthesis A. has not been well-studied. In this study, we identified YABBY family transcription factor, AaYABBY5, positive regulator anthocyanin total contents annua. AaYABBY5 was selected based on its similar expression pattern to the phenylalanine ammonia lyase (PAL), chalcone synthase...
Summary Artemisinin, a sesquiterpene compound synthesized and stored in the glandular trichome of Artemisia annua leaves, has been used to treat malaria. Previous studies have shown that both light jasmonic acid (JA) can promote biosynthesis artemisinin, promotion artemisinin by JA is dependent on light. However, specific molecular mechanism remains unclear. Here, we report MYB transcription factor, AaMYB108, identified from transcriptome analysis treatment, as positive regulator A. ....
Glandular secretory trichomes (GSTs) can secrete and store a variety of specific metabolites. By increasing GST density, valuable metabolites be enhanced in terms productivity. However, the comprehensive detailed regulatory network initiation still needs further investigation. screening complementary DNA library derived from young leaves Artemisia annua, we identified MADS-box transcription factor, AaSEPALLATA1 (AaSEP1), that positively regulates initiation. Overexpression AaSEP1 A. annua...
Abstract The breeding of crops with improved nitrogen use efficiency (NUE) is crucial for sustainable agriculture, but the involvement epigenetic modifications remains unexplored. Here, we analyze chromatin landscapes two wheat cultivars (KN9204 and J411) that differ in NUE under varied conditions. expression metabolism genes closely linked to variation histone modification instead differences DNA sequence. Epigenetic exhibit clear cultivar-specificity, which likely contributes distinct...
Summary WUSCHEL ( WUS ) is critical for plant meristem maintenance and determinacy in Arabidopsis, the regulation of its spatiotemporal expression patterns complex. We previously found that AGAMOUS AG ), a key MADS ‐domain transcription factor floral organ identity determinacy, can directly suppress through recruitment Polycomb group (PcG) protein TERMINAL FLOWER 2 TFL 2, also known as LIKE HETEROCHROMATIN PROTEIN 1, LHP 1) at locus; however, mechanism by which repressed remains unclear....
Kernel number per spike determined by the or inflorescence development is one important agricultural trait for wheat yield that critical global food security. While a few genes were identified, genetic regulatory mechanism underlying supernumerary spikelets (SSs) still unclear. Here, we cloned FRIZZY PANICLE (WFZP) gene from local cultivar. WFZP specifically expressed at sites where spikelet meristem and floral are initiated, which differs expression patterns of its homologs FZP/BD1 in rice...
Artemisia annua, a traditional Chinese medicinal plant, remains the only plant source for artemisinin production, yet few genes have been identified to be involved in both response biotic stresses, such as pathogens, and biosynthesis. Here, we isolated WRKY transcription factor (TF) AaWRKY17, which could significantly increase content resistance Pseudomonas syringae A. annua. Yeast one-hybrid (Y1H), dual-luciferase (dual-LUC), electrophoretic mobility shift assay (EMSA) results showed that...
Artemisinin is a secondary metabolite extracted from Artemisia annua. As an effective antimalarial component certified by WHO, artemisinin has extensive economical values. Numerous studies about transcription factors positively regulating biosynthesis have been published while negative regulators are rarely reported. In the present study, we identified AaMYB15 as first R2R3-MYB that negatively regulates in A. Experimental evidences showed factor within nucleus and predominantly expressed...
Abstract Low light stress seriously decreased wheat grain number through the formation of aborted spike during reproductive period and induced new tiller regeneration to offset loss number. However, mechanism by which plants coordinate growth tillers remains unknown. To better understand this coordinated process, morphological, physiological transcriptomic analyses were performed under low at young microspore stage. Our findings indicated that leaves exhausted most stored carbohydrates in 1...
Since the advent of agriculture, more than one-fourth Earth's land surface has been converted for agricultural purposes, which conversion from natural to landscapes dramatically changes plant communities that are integral ecosystem processes. By developing perennial crops through breeding would help deal with multiple issues involving environmental conservation and food security in a world shrinking resources. It can provide services essential sustainable production effectively systems based...