A5070394658- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Cancer therapeutics and mechanisms
- Plant tissue culture and regeneration
- Microbial Natural Products and Biosynthesis
- Traditional Chinese Medicine Analysis
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Genomics and Phylogenetic Studies
- Insect Resistance and Genetics
- Natural Fiber Reinforced Composites
- Lignin and Wood Chemistry
- Insect-Plant Interactions and Control
- Ginseng Biological Effects and Applications
- Plant nutrient uptake and metabolism
- Bamboo properties and applications
- Neurobiology and Insect Physiology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Wood Treatment and Properties
- Malaria Research and Control
- Biochemical and biochemical processes
- Pharmacological Effects of Natural Compounds
- Ubiquitin and proteasome pathways
- Natural product bioactivities and synthesis
- Phytochemical Studies and Bioactivities
Zhejiang Chinese Medical University
2015-2024
Blood Center of Zhejiang Province
2024
Ministry of Agriculture and Rural Affairs
2022-2024
South China Agricultural University
2022-2024
China Academy of Chinese Medical Sciences
2024
Shanghai Innovative Research Center of Traditional Chinese Medicine
2024
China Academy of Launch Vehicle Technology
2023
Key Laboratory of Guangdong Province
2023
Lanzhou University
2023
Shanghai Jiao Tong University
2015-2022
Glandular trichomes are generally considered biofactories that produce valuable chemicals. Increasing glandular trichome density is a very suitable way to improve the productivity of these metabolites, but little known about regulation formation. Phytohormone jasmonate (JA) promotes initiation in various plants, its mechanism also unknown. By searching transcription factors regulated by JA Artemisia annua, we identified novel homeodomain-leucine zipper factor, HOMEODOMAIN PROTEIN 1 (AaHD1),...
Summary Artemisinin is a type of sesquiterpene lactone well known as an antimalarial drug, and specifically produced in glandular trichomes Artemisia annua . However, the regulatory network for artemisinin biosynthetic pathway remains poorly understood. Exploration trichome‐specific transcription factors would facilitate elucidation mechanism biosynthesis. The WRKY factor GLANDULAR TRICHOME ‐ SPECIFIC 1 ( Aa GSW ) was cloned analysed A. exhibited similar expression patterns to genes AP 2/...
Tanshinone is a group of active diterpenes, which are widely used in the treatment cardiovascular disease. In this study, methyl jasmonate (MJ) and salicylic acid (SA) were to investigate their effects on tanshinone accumulation biosynthetic gene expression hairy roots geranylgeranyl diphosphate synthase (SmGGPPS) overexpression line (G50) Salvia miltiorrhiza. High-performance liquid chromatography analysis showed that total content G50 was obviously increased by 3.10-fold (11.33 mg/g) with...
Bunge is an herb rich in bioactive tanshinone and salvianolic acid compounds. It primarily used as effective medicine for treating cardiovascular cerebrovascular diseases. Liposoluble tanshinones water-soluble phenolic acids are a series of terpenoids compounds, respectively. However, the regulation mechanism simultaneous promotion biosynthesis remains unclear. This study identified R2R3-MYB subgroup 20 transcription factor (TF),
Summary The glandular secretory trichomes ( GST s) on Artemisia annua leaves have the capacity to secrete and store artemisinin, a compound which is most effective treatment for uncomplicated malaria. An strategy improve artemisinin content therefore increase density of s in A. . However, formation mechanism remains poorly understood. To explore mechanisms initiation , we screened myeloblastosis MYB ) transcription factor genes from transcriptome database identified MIXTA factor, Aa 1,...
JA-induced JAZ8 degradation releases and activates the TCP14-ORA complex in enhancing artemisinin biosynthesis Artemisia annua .
Tanshinones, one group of bioactive diterpenes, were widely used in the treatment cardiovascular diseases. WRKYs play important roles plant metabolism, but their regulation mechanism S. miltiorrhiza remains elusive. In this study, WRKY transcription factor SmWRKY1 was isolated and characterized from miltiorrhiza. Multiple sequence alignment phylogenetic tree analysis showed shared high homology with other such as CrWRKY1. predominantly expressed leaves stems, responsive to salicylic acid...
The NAC (NAM, ATAF and CUC) superfamily is one of the largest plant-specific transcription factor families. factors always play important roles in response to various abiotic stresses. A gene AaNAC1 containing a complete open reading frame (ORF) 864 bp was cloned from Artemisia annua. expression could be induced by dehydration, cold, salicylic acid (SA) methyl jasmonate (MJ), suggesting that it might key regulator stress signaling pathways A. shown localized nuclei transforming tobacco leaf...
Artemisinin, the frontline drug against malaria, is a sesquiterpenoid extracted from Artemisia annua. Light has been proposed to play an important role in activation of artemisinin biosynthesis. Here, we report basic leucine zipper transcription factor (TF) AaHY5 as key regulator light-induced biosynthesis artemisinin. We show that overlaps with genes response light and A. annua tissues. Analysis overexpression RNAi-suppression lines suggests positive expression accumulation complements hy5...
Abstract The limited bioavailability of plant-derived natural products with anticancer activity poses major challenges to the pharmaceutical industry. An example this is camptothecin, a monoterpene indole alkaloid potent that extracted at very low concentrations from woody plants. Recently, camptothecin biosynthesis has been shown become biotechnologically amenable in hairy-root systems producer Ophiorrhiza pumila . Here, time-course expression and metabolite analyses were performed identify...
Summary Artemisinin, a sesquiterpene lactone widely used in malaria treatment, was discovered the medicinal plant Artemisia annua . The biosynthesis of artemisinin is efficiently regulated by jasmonate (JA) and abscisic acid (ABA) via regulatory factors. However, mechanisms linking JA ABA signalling with through an associated network downstream transcription factors (TFs) remain enigmatic. Here we report AaTCP15, dual‐responsive teosinte branched1/cycloidea/proliferating (TCP) TF, which...
The low comprehensive properties of soy protein-based wood adhesives (SPWAs), such as weak bonding strength, poor water and mildew resistance, etc., are the main issues that severely restrict their wide application in manufacturing industry. In this study, fully biobased SPWAs with excellent flame retardancy were developed via a facile cross-linking reaction between protein isolate (SPI), furfuryl alcohol (FA), phytic acid (PA). Because SPI, FA, PA all derived from green sustainable biomass...
Abstract Artemisinin, also known as ‘Qinghaosu’, is a chemically sesquiterpene lactone containing an endoperoxide bridge. Due to the high activity kill Plasmodium parasites, artemisinin and its derivatives have continuously served foundation for antimalarial therapies. Natural unique traditional Chinese medicinal plant Artemisia annua L., content in this low. This has motivated synthesis of bioactive compound using yeast, tobacco, Physcomitrium patens systems. However, production these...
Artemisinin is a sesquiterpene lactone endoperoxide extracted from traditional Chinese medicinal plant Artemisia annua. Artemisinin-based combination therapies (ACTs) are recommended as the best treatment of malaria by World Health Organization (WHO). Both phytohormone jasmonic acid (JA) and light promote artemisinin biosynthesis in A. Interestingly, we found that increase JA was dependent on light. However, relationship between two signal pathways mediated remains unclear. Here, collected...
Abstract Artemisinin is a sesquiterpene lactone produced by the Chinese traditional herb Artemisia annua and used for treatment of malaria. It known that salicylic acid (SA) can enhance artemisinin content but mechanism which it does so not known. In this study, we systematically investigated basic leucine zipper family transcription factor, AaTGA6, involved in SA signaling to regulate biosynthesis. We found specific vivo vitro binding AaTGA6 protein ‘TGACG’ element AaERF1 promoter....
Abstract Glandular secreting trichomes (GSTs) synthesize and secrete large quantities of secondary metabolites, some which have well-established commercial value. An example is the anti-malarial compound artemisinin, synthesized in GSTs Artemisia annua. Accordingly, there considerable interest understanding processes that regulate GST density as a strategy to increase artemisinin production. In this study, we identified GST-specific WRKY transcription factor from A. annua, AaGSW2, positively...
Artemisia annua, a traditional Chinese medicinal plant, remains the only plant source for artemisinin production, yet few genes have been identified to be involved in both response biotic stresses, such as pathogens, and biosynthesis. Here, we isolated WRKY transcription factor (TF) AaWRKY17, which could significantly increase content resistance Pseudomonas syringae A. annua. Yeast one-hybrid (Y1H), dual-luciferase (dual-LUC), electrophoretic mobility shift assay (EMSA) results showed that...
Camptothecin is a chemotherapeutic drug widely used to treat various cancers. Ophiorrhiza pumila an ideal plant model for the study of camptothecin production, with advantages studying biosynthesis and regulation. The DNA-binding WRKY transcription factors have key regulatory role in secondary metabolite plants. However, little currently known about their involvement O. pumila. We identified 46 OpWRKY genes unevenly distributed on 11 chromosomes Phylogenetic multiple sequence alignment...
Artemisinin, a sesquiterpenoid endoperoxide, isolated from the plant Artemisia annua L., is widely used in treatment of malaria. Another sesquiterpenoid, β-caryophyllene having antibiotic, antioxidant, anticarcinogenic and local anesthetic activities, also presented A. annua. The role played by sesquiterpene transporters trichomes accumulation these metabolites poorly understood other species. We identified AaPDR3, encoding pleiotropic drug resistance (PDR) transporter located to plasma...