A5051812296- Plant biochemistry and biosynthesis
- Fungal Infections and Studies
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Antifungal resistance and susceptibility
- Insect Resistance and Genetics
- Traditional Chinese Medicine Analysis
- Photosynthetic Processes and Mechanisms
- Plant Molecular Biology Research
- Cancer-related molecular mechanisms research
- Nail Diseases and Treatments
- Natural product bioactivities and synthesis
- Plant-derived Lignans Synthesis and Bioactivity
- CRISPR and Genetic Engineering
- Plant Virus Research Studies
- Plant Pathogens and Fungal Diseases
- Cytomegalovirus and herpesvirus research
- Plant Parasitism and Resistance
- RNA Research and Splicing
- RNA modifications and cancer
- Genomics, phytochemicals, and oxidative stress
- Autoimmune Bullous Skin Diseases
- Microbial Natural Products and Biosynthesis
- Phytochemistry and Biological Activities
- Corneal surgery and disorders
Shanghai Changzheng Hospital
2016-2025
Chengdu Institute of Biology
2021-2025
Chinese Academy of Sciences
2002-2025
YangPu Geriatric Hospital
2025
Nankai University
2025
Second Military Medical University
2014-2024
University of Bern
2024
University Hospital of Bern
2024
Shaanxi Provincial People's Hospital
2021-2024
Shanghai University
2020-2024
T-helper (Th) 22 is involved in the pathogenesis of inflammatory diseases. The roles Th22 cells pathophysiological ankylosing spondylitis (AS) and rheumatoid arthritis (RA) remain unsettled. So we examined frequencies cells, Th17 Th1 peripheral blood (PB) from patients with AS RA compared both healthy controls as well osteoarthritis.We studied 32 patients, 20 10 OA controls. expression IL-22, IL-17 IFN-γ were AS, RA, by flow cytometry. Plasma IL-22 levels enzyme-linked immunosorbent...
The aim of this work was to examine rosmarinic acid and its derivative lithospermic B accumulation, as well related gene transcript metabolite profiling in Salvia miltiorrhiza Bunge (Lamiacae) hairy root cultures, response methyl jasmonate (0.1 mM). Results showed dramatically enhanced both from approximately 3.25 6.02%, 2.94 19.3% dry weight, respectively. Meantime, several biosynthetic transcripts were coordinately induced, with phenylalanine ammonia-lyase, cinnamic 4-hydroxylase, tyrosine...
Rational engineering to produce biologically active plant compounds has been greatly impeded by our poor understanding of the regulatory and metabolic pathways underlying biosynthesis these compounds. Here we capitalized on previously described gene-to-metabolite network in order engineer rosmarinic acid (RA) pathway for production beneficial RA lithospermic B (LAB) Salvia miltiorrhiza hairy root cultures. Results showed their was elevated (1) overexpression single gene, including cinnamic...
Salvia miltiorrhiza Bunge, which contains tanshinones and phenolic acids as major classes of bioactive components, is one the most widely used herbs in traditional Chinese medicine. Production enhanced by methyl jasmonate (MeJA). Transcription factor MYC2 switch jasmontes signaling plants. Here, we focused on two novel JA-inducible genes S. miltiorrhiza, designated SmMYC2a SmMYC2b, were localized nucleus. SmMYC2b also discovered to interact with SmJAZ1 SmJAZ2, implying that MYC2s might...
The important antimalarial drug artemisinin is biosynthesized and stored in Artemisia annua glandular trichomes the content correlates with trichome density; however, factors affecting development are largely unknown. Here, we demonstrate that A. R2R3 MYB transcription factor TrichomeLess Regulator 1 (TLR1) negatively regulates development. In annua, TLR1 overexpression lines had 44.7%-64.0% lower density 11.5%-49.4% contents TLR1-RNAi 33%-93.3% higher 32.2%-84.0% compared non-transgenic...
Summary Salvianolic acids (SA), such as rosmarinic acid (RA), danshensu (DSS), and their derivative salvianolic B (SAB), etc. widely existed in Lamiaceae Boraginaceae families, are of interest due to medicinal properties the pharmaceutical industries. Hundreds studies past decades described that 4‐coumaroyl‐CoA 4‐hydroxyphenyllactic (4‐HPL) common substrates biosynthesize SA with participation synthase (RAS) cytochrome P450 98A (CYP98A) subfamily enzymes different plants. However, our recent...
Posttraumatic stress disorder (PTSD) is associated with decreased activity in the dorsolateral prefrontal cortex (DLPFC), brain region that regulates working memory and preparation selection of fear responses.We investigated gene expression profiles DLPFC Brodmann area (BA) 46 postmortem patients (n=6) without PTSD using human mitochondria-focused cDNA microarrays.Our study revealed PTSD-specific fingerprints 800 informative genes across all these 12 BA46 samples, 119 (±>1.25,p<0.05) 42...
Abstract Artemisinin is a sesquiterpene lactone produced by the Chinese traditional herb Artemisia annua and used for treatment of malaria. It known that salicylic acid (SA) can enhance artemisinin content but mechanism which it does so not known. In this study, we systematically investigated basic leucine zipper family transcription factor, AaTGA6, involved in SA signaling to regulate biosynthesis. We found specific vivo vitro binding AaTGA6 protein ‘TGACG’ element AaERF1 promoter....
Glandular secretory trichomes (GSTs) are regarded as biofactories for synthesizing, storing, and secreting artemisinin. It is necessary to figure out the initiation development regulatory mechanism of GSTs cultivate high-yielding Artemisia annua. Here, we identified an MYB transcription factor, AaTAR2, from bioinformatics analysis A. annua genome database Arabidopsis trichome development-related genes. AaTAR2 mainly expressed in young leaves located nucleus. Repression overexpression...
Until recently, many studies on the role of phytohormones in plant secondary metabolism focused jasmonic acid (JA), salicylic (SA), gibberellins (GA), and abscisic (ABA). It is now clear that phytohormone-induced regulation signaling occurs via biosynthetic pathway genes at transcriptional level or through posttranslational regulation, an increase metabolite deposition (e.g., trichomes). Here, we summarize recent advances, updating current reports molecular machinery JA, SA, GA, ABA involved...
Biological control of chestnut blight caused by the filamentous ascomycete Cryphonectria parasitica can be achieved with a virus that infects this fungus. This hypovirus causes perturbation fungal development results in low virulence (hypovirulence), poor asexual sporulation, and female infertility without affecting growth culture. At molecular level, is known to affect transcription number genes. Two these genes,Vir1 Vir2, produce abundant transcripts noninfected strains fungus, but are not...
Isatis indigotica is a widely used herb for the clinical treatment of colds, fever, and influenza in Traditional Chinese Medicine (TCM). Various structural classes compounds have been identified as effective ingredients. However, little known at genetics level about these active metabolites. In present study, we performed de novo transcriptome sequencing first time to produce comprehensive dataset I. indigotica. A database 36,367 unigenes (average length = 1,115.67 bases) was generated by...
Isatis indigotica Fort. is one of the most commonly used traditional Chinese medicines. Its antiviral compound a kind lignan, which formed with action dirigent proteins (DIR). DIR are members large family impart stereoselectivity on phenoxy radical-coupling reaction, yielding optically active lignans from two molecules E-coniferyl alcohol. They exist in almost every vascular plant. However, and DIR-like protein gene I. has not been analyzed detail yet. This study focuses discovery analysis...
Atropa belladonna is officially deemed as the commercial plant to produce scopolamine in China. In this study we report simultaneous overexpression of two functional genes involved biosynthesis scopolamine, which encode upstream key enzyme putrescine N-methyltransferase (PMT) and downstream hyoscyamine 6β-hydroxylase (H6H), respectively, transgenic herbal plants belladonna. Analysis gene expression profile indicated that both pmt h6h were expressed at a higher level lines, would be favorable...
A molecular description of lignan biosynthesis in Isatis indigotica displaying its synthetic characteristics and regulatory mechanism is great importance for the improvement production this class active compounds. To discover potential key catalytic steps genes, I. hairy roots elicited by methyl jasmonate (MeJA) were used as a source systematic variation exploring metabolic/transcriptional changes candidate genes that might play roles biosynthesis. The reprogramming modulated MeJA was...
Fifteen genes were predicted to be closely related safranal and crocin production by multi-omic analysis in which CsALDH3 was validated used construct crocetin-producing yeast.
Abstract Polygonatum cyrtonema Hua (Huangjing, HJ) has medicinal and edible value in China. However, the seeds of this plant are naturally difficult to germinate. Therefore, elucidate mechanism underlying germination order meet market demand, metabolomic transcriptomic analyses were performed study. We observed that hormones α-amylase activity differentially regulated when comparing germinated un-germinated seeds. In addition, metabolites related phenylpropanoid flavonoid biosynthesis...