A5052449098- Plant biochemistry and biosynthesis
- CRISPR and Genetic Engineering
- Pharmacological Effects of Natural Compounds
- Cancer Research and Treatments
- Alkaloids: synthesis and pharmacology
- Plant tissue culture and regeneration
- Plant responses to water stress
- Transgenic Plants and Applications
- Quinazolinone synthesis and applications
- Biochemical and biochemical processes
- Molecular Biology Techniques and Applications
- Malaria Research and Control
- Virus-based gene therapy research
- Genomics and Phylogenetic Studies
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Analytical Chemistry and Chromatography
- Animal Genetics and Reproduction
- Lipid metabolism and biosynthesis
- Plant Gene Expression Analysis
- Berberine and alkaloids research
- RNA Research and Splicing
- Chromatography in Natural Products
- Insect Resistance and Genetics
- Plant Parasitism and Resistance
Fujian Normal University
2022-2023
Huaqiao University
2016-2023
Nantong University
2022
Second Military Medical University
2016-2019
Shanghai Changzheng Hospital
2016
Sichuan University
2005
Abstract Artemisinin is a sesquiterpene lactone produced by the Chinese traditional herb Artemisia annua and used for treatment of malaria. It known that salicylic acid (SA) can enhance artemisinin content but mechanism which it does so not known. In this study, we systematically investigated basic leucine zipper family transcription factor, AaTGA6, involved in SA signaling to regulate biosynthesis. We found specific vivo vitro binding AaTGA6 protein ‘TGACG’ element AaERF1 promoter....
Summary MicroRNAs (miRNAs) play crucial roles in plant development and secondary metabolism through different modes of sequence‐specific interaction with their targets. Artemisinin biosynthesis is extensively regulated by phytohormones. However, the function phytohormone‐responsive miRNAs artemisinin remains enigmatic. Thus, we combined analysis transcriptomics, small RNAs, degradome to generate a comprehensive resource for identifying key miRNA‐target circuits involved phytohormone‐induced...
Abstract Traditional Chinese Medicine (TCM) has been developed for thousands of years and formed an integrated theoretical system based on a large amount clinical practice. However, essential ingredients in TCM herbs have not fully identified, their precise mechanisms targets are elucidated. In this study, new strategy combining comprehensive two-dimensional K562/cell membrane chromatographic silico target identification was established to characterize active components from Indigo naturalis...
Baphicacanthus cusia (Nees) Bremek, the plant source for many kinds of drugs in traditional Chinese medicine, is widely distributed South China, especially Fujian. Recent studies about B. mainly focus on its chemical composition and pharmacological effects, but further analysis plant's gene functions expression required to better understand synthesis effective compounds. Real-time quantitative polymerase chain reaction (RT-qPCR) a powerful method analysis. It necessary select suitable...
Under hypoxic conditions, the expression of Vitreoscilla hemoglobin (VHb) in plants is proposed to increase productivity certain oxygen-requiring metabolic pathways by promoting delivery oxygen. Tropane alkaloids (TAs) are a class important plant secondary metabolites with significant medicinal value; final step their biosynthesis requires Whether heterologous VHb, especially different subcellular compartments, can accelerate accumulation TAs not known. Herein, effect VHb locations on TA...
Baphicacanthus cusia (Nees) Bremek ( B. ) is an essential traditional Chinese herb that commonly used to treat colds, fever, and influenza. Indole alkaloids, such as indigo indirubin, are the primary active constituents of . The indole-producing reaction crucial for regulating flow indole alkaloids metabolites along pathways coordinating secondary product biosynthesis in plants. tryptophan synthase alpha-subunit (TSA) can catalyse a process produces indole, which free enter metabolite...
Artemisinin is the most practical medication for treatment of malaria, but only very minimally synthesized in Artemisia annua, significantly less than market needs. In this study, indole-3-acetic acid (IAA) was used to investigate its effects on trichomes, artemisinin accumulation, and biosynthetic gene expression A. anuua. The results showed that exogenous IAA could contribute growth development annua increase density trichomes. Analysis using liquid chromatography-tandem mass spectrometry...
Baphicacanthus cusia (Nees) Bremek ( B. ) is an important medicinal plant. Its effective substances including indigo and indirubin are metabolites in indoleacetate metabolic pathway. Based on a previous transcriptome sequencing analysis, WRKY transcription factor, BcWRKY1, was identified, showing significant correlation with from . In this study, BcWRKY1 cloned by reverse transcription-polymerase chain reaction (RT-PCR). Further analysis showed that the gene 916 bp length, containing three...