A5088165302- Traditional Chinese Medicine Analysis
- Analytical Chemistry and Chromatography
- Chromatography in Natural Products
- Metabolomics and Mass Spectrometry Studies
- Natural product bioactivities and synthesis
- Plant-based Medicinal Research
- Pharmacological Effects of Natural Compounds
- Microfluidic and Capillary Electrophoresis Applications
- Phytochemistry and Biological Activities
- Ginseng Biological Effects and Applications
- Phytochemical Studies and Bioactivities
- Drug Transport and Resistance Mechanisms
- Computational Drug Discovery Methods
- Flavonoids in Medical Research
- Monoclonal and Polyclonal Antibodies Research
- Berberine and alkaloids research
- Traditional Chinese Medicine Studies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Pharmaceutical Quality and Counterfeiting
- Antifungal resistance and susceptibility
- Phytochemistry and biological activity of medicinal plants
- Cancer, Hypoxia, and Metabolism
- Glycosylation and Glycoproteins Research
- Medicinal Plant Pharmacodynamics Research
- Bioactive Natural Diterpenoids Research
Second Military Medical University
2016-2025
Chongqing Medical University
2025
Mayo Clinic in Arizona
2023
Shanghai Research Institute of Chemical Industry
2011-2022
Fujian University of Traditional Chinese Medicine
2020
State Council of the People's Republic of China
2017
Shanghai Changzheng Hospital
2016
Gansu University of Traditional Chinese Medicine
2014
Shanghai Institute of Materia Medica
2013
Nanjing General Hospital of Nanjing Military Command
2013
Lianhuaqingwen (LHQW) capsule, a herb medicine product, has been clinically proved to be effective in coronavirus disease 2019 (COVID-19) pneumonia treatment. However, human exposure LHQW components and their pharmacological effects remain largely unknown. Hence, this study aimed determine anti-COVID-19 activities. Analysis of component profiles plasma urine after repeated therapeutic dosing was conducted using combination HRMS an untargeted data-mining approach, leading detection 132...
Hepatocellular carcinoma (HCC) is one of the most common malignant tumors in world. However, current biomarkers that discriminate HCC from liver cirrhosis (LC) are important but limited. More reliable for diagnosis therefore needed. Serum patients, LC patients and healthy volunteers were analyzed using NMR LC/MS-based approach conjunction with random forest (RF) analysis to their serum metabolic profiles. Thirty-two potential have been identified, feasibility these was evaluated, where 100%...
Cell membrane chromatography (CMC) derived from pathological tissues is ideal for screening specific components acting on diseases complex medicines owing to the maximum simulation of in vivo drug-receptor interactions. However, there are no tissue-derived CMC models that have ever been developed, as well visualized affinity comparison potential active between normal and columns. In this study, a novel comparative normal/failing rat myocardium analysis system based online column selection...
Astragali Radix (AR) is a clinically used herbal medicine with multiple immunomodulatory activities that can strengthen the activity and cytotoxicity of natural killer (NK) cells. However, owing to complexity its composition, specific active ingredients in AR act on NK cells are not clear yet. Cell membrane chromatography (CMC) mainly screen complex system medicines. In this study, new comprehensive two-dimensional (2D) NK-92MI CMC/C18 column/time-of-flight mass spectrometry (TOFMS) was...
Abstract High‐performance liquid chromatography‐diode array detection (HPLC‐DAD), electrospray ionization time‐of‐flight mass spectrometry (HPLC‐ESI‐TOF‐MS) and quadrupole ion trap (HPLC‐ESI‐MS n ) were used for the isolation, identification structural analysis of water‐soluble phenolic nonpolar diterpenoid constituents in Dan‐shen (Radix Salvia miltiorrhizae which was prepared by sonication 70% methanol. Mass spectra obtained ESI‐TOF‐MS (ESI‐QIT‐MS). A formula database known established...
Background In spite of great advances in target-oriented Western medicine for treating myocardial infarction (MI), it is still a leading cause death worldwide epidemic. contrast to medicine, Traditional Chinese (TCM) uses holistic and synergistic approach restore the balance Yin-Yang body energy so body's normal function can be restored. Sini decoction (SND) well-known formula TCM which has been used treat MI many years. However, its activity evaluation mechanistic understanding are lacking...
Abstract Identifying the molecular targets for beneficial effects of active small-molecule compounds simultaneously is an important and currently unmet challenge. In this study, we firstly proposed network analysis by integrating data from pharmacology metabolomics to identify components in sini decoction (SND) against heart failure. To begin with, 48 potential SND failure were predicted serum pharmacochemistry, text mining similarity match. Then, employed including docking these components....
A panel of serum metabolite markers (glutamine, kynurenine, and LysoPC(18:2)) was identified as candidate diagnostic biomarkers for post-stroke cognitive impairment.
Cell membrane chromatography (CMC) is an ideal method for screening potential active components acting on target cell membranes from a complex system, such as herbal medicines. But due to the decay and falling-off of membranes, CMC column suffers short life span low reproducibility. This has greatly limited application this model, especially when materials are hard obtain. To solve problem, novel type (3-aminopropyl)triethoxysilane (APTES)-decorated silica gel was employed. The decorated...
Cell membrane affinity chromatography has been widely applied in protein (MP)-targeted drug screening and interaction analysis. However, current methods, the MP sources are derived from cell lines or recombinant expression, which time-consuming for culture purification, also difficult to ensure purity consistent orientation of MPs chromatographic stationary phase. In this study, a novel situ synthesis (iSMAC) method was developed utilizing cell-free expression (CFE) covalent immobilized...
Adulteration of botanical dietary supplements with prohibited synthetic drugs has become a serious problem. In this paper, method for testing used to adulterate was developed using liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) coupled linearity ion-trap system in the multiple reaction monitoring (MRM) plus enhanced product ion (EPI) mode. Twenty-three exhibiting various pharmacological effects, comprising blood pressure and lipid-lowering agents,...
Background Doxorubicin (DOX) is one of the most potent antitumor agents available; however, its clinical use limited because risk severe cardiotoxicity. Though numerous studies have ascribed DOX cardiomyopathy to specific cellular pathways, precise mechanism remains obscure. Sini decoction (SND) a well-known formula Traditional Chinese Medicine (TCM) and considered as efficient against DOX-induced cardiomyopathy. However, action mechanisms are not well known due complex components....
Biofilm-associated infections are difficult to treat because of their decreased susceptibility antimicrobial therapy. Candida albicans is the most common fungal pathogen associated with colonization and biofilm formation on surfaces indwelling medical devices which show intrinsic resistance many commonly used antifungal agents. In this study, a metabonomic method using gas chromatography-mass spectrometry (GC/MS) was developed characterize metabolic profiles during whole developmental phases...
Tumor necrosis factor α (TNF-α) is overexpressed in various diseases, and it has been a validated therapeutic target for autoimmune diseases. All therapeutics currently used to TNF-α are biomacromolecules, limited numbers of chemical inhibitors have reported, which makes the identification small-molecule alternatives an urgent need. Recent studies mainly focused on identifying small molecules that directly bind or TNF receptor-1 (TNFR1), inhibit interaction between TNFR1, and/or regulate...
Liver fibrosis and its endstage, cirrhosis, represent a major public health problem worldwide. Activation of hepatic stellate cells (HSCs) is central event in fibrosis. However, the proteins that control HSC activation are incompletely understood. Here we show (6aS, 10S, 11aR, 11bR, 11cS)-10-methylamino-dodecahydro-3a, 7a-diaza-benzo [de]anthracene-8-thione (MASM) exhibits potent inhibitory activity against liver vitro vivo associated with reduction Akt phosphorylation. Furthermore,...