A5101879701- Traditional Chinese Medicine Analysis
- Plant-based Medicinal Research
- Chromatography in Natural Products
- Natural product bioactivities and synthesis
- Pharmacological Effects of Natural Compounds
- Bioactive Natural Diterpenoids Research
- Cancer-related molecular mechanisms research
- Phytochemical Studies and Bioactivities
- Phytochemistry and Biological Activities
- Metabolomics and Mass Spectrometry Studies
- MicroRNA in disease regulation
- Drug-Induced Hepatotoxicity and Protection
- Plant-derived Lignans Synthesis and Bioactivity
- Circular RNAs in diseases
- RNA Research and Splicing
- Alkaloids: synthesis and pharmacology
- Advanced Chemical Sensor Technologies
- Phytochemistry and Bioactive Compounds
- Phytochemistry and biological activity of medicinal plants
- Berberine and alkaloids research
- Signaling Pathways in Disease
- Ginseng Biological Effects and Applications
- Analytical Chemistry and Chromatography
- Lung Cancer Treatments and Mutations
- Bioactive Compounds and Antitumor Agents
North University of China
2023-2024
Qingdao University
2021-2022
Affiliated Hospital of Qingdao University
2021-2022
Shanghai Clinical Research Center
2022
Shanghai University
2022
First Affiliated Hospital of GuangXi Medical University
2017-2021
Guangxi Medical University
2017-2021
Capital Medical University
2011-2021
Beijing Friendship Hospital
2021
Second Military Medical University
2009-2018
Oral gene therapy has emerged as a potential optimal treatment for ulcerative colitis (UC). Nucleic acid drugs possessing versatility can not only inhibit inflammation but realize colon mucosal healing, fulfilling the clinical objective of UC therapy. However, effective accumulation and distribution oral nucleic in remain considerable challenge. Furthermore, current delivery systems pay more attention to colon, while which plays key role treatment, never catches researchers. Here, we used...
Abstract High‐performance liquid chromatography‐diode array detection (HPLC‐DAD), electrospray ionization time‐of‐flight mass spectrometry (HPLC‐ESI‐TOF‐MS) and quadrupole ion trap (HPLC‐ESI‐MS n ) were used for the isolation, identification structural analysis of water‐soluble phenolic nonpolar diterpenoid constituents in Dan‐shen (Radix Salvia miltiorrhizae which was prepared by sonication 70% methanol. Mass spectra obtained ESI‐TOF‐MS (ESI‐QIT‐MS). A formula database known established...
Cell membrane chromatography (CMC) is an ideal method for screening potential active components acting on target cell membranes from a complex system, such as herbal medicines. But due to the decay and falling-off of membranes, CMC column suffers short life span low reproducibility. This has greatly limited application this model, especially when materials are hard obtain. To solve problem, novel type (3-aminopropyl)triethoxysilane (APTES)-decorated silica gel was employed. The decorated...
Accumulating evidence shows that tRNA-derived fragments are a novel class of functional small non-coding RNA; however, their roles in aortic dissection (AD) still unknown. In this study, we found 5′-tiRNA-Cys-GCA was significantly downregulated human and mouse models dissection. The abnormal proliferation, migration, phenotypic transition vascular smooth muscle cells (VSMCs) played crucial role the initiation progression dissection, with as potential switching regulator, because its...
Compared to the use of anti-TB drugs for treatment tuberculosis (TB), surgery is destructive and associated with higher risk. Whether needed TB-destroyed lungs (for short: destroyed lungs) still controversial unresolved in clinic. Retrospective analysis efficacy was performed on 172 cases (176 surgeries) Thoracic Surgery Department our hospital from April 1992 June 2010. A total 83 male 89 female patients analyzed. The youngest patient 7 years old, oldest 72 a mean age 38.4 years. 120 had...
Abstract This study explored the therapeutic effect of bone marrow mesenchymal stem cell‐derived exosomes on treatment obesity‐induced fracture healing. Quantitative real‐time PCR was used to detect expression lncRNA H19, miR‐467 and Hoxa10 combined with WB detection osteogenic markers (RUNX2, OPN, OCN). Determine whether have entered BMSCs by immunofluorescence staining. Alkaline phosphatase (ALP) alizarin red staining (ARS) were ALP activity calcium deposition. We found that high‐fat can...
Schisandra chinensis (S. chinensis) is a traditional Chinese herbal medicine widely used for the treatment of liver disease, whose main active components are lignans. However, action mechanisms lignans in S. remain unclear. This study aimed to investigate protective effect and related molecular mechanism lignan extract (SLE) against carbon tetrachloride- (CCl4-) induced acute injury mice. Different doses SLE at 50, 100, 200 mg/kg were administered daily by gavage 5 days before CCl4...
Abstract High‐performance liquid chromatography with diode‐array detection (HPLC/DAD), time‐of‐flight mass spectrometry (HPLC/TOFMS) and quadrupole ion trap (HPLC/QITMS) were used for separation, identification structural analysis of furocoumarins in Angelica dahurica . Two (imperatorin isoimperatorin) extract identified unambiguously by comparing their relative retention times, characteristic ultraviolet information accurate measurement. A formula database known was established, against...
A liquid chromatography–mass spectrometry method has been developed and validated for rapid simultaneous determination of the oleanolic ursolic acid contents in rat plasma with betulinic as internal standard (IS). The samples were prepared by a liquid–liquid extraction procedure. Chromatographic separation was performed Chromasil-C18 column (250 mm × 4.6 mm, i.d. 5 μm) methanol–water mobile phase at 1 mL/min. detection accomplished under selected-ion-monitoring mode negative electrospray...
Hypaconitine (HC) is one of the main aconitum alkaloids in Aconitum carmichaelii (AC), which considered to be effective on cardiovascular disease, although it also has high toxicity. Sini Decoction (SND), composed carmichaelii, Glycyrrhiza uralensis and Zingiber officinale, a traditional Chinese multi-herbal formula for recuperating depleted yang. The aim this study was compare pharmacokinetics HC rat plasma after oral administration HC, AC extract SND, investigate effect other two herbal...
High-performance liquid chromatography/diode-array detection (HPLC/DAD), time-of-flight mass spectrometry (HPLC/TOFMS) and quadrupole ion trap (HPLC/QIT-MS) were used for separation, identification structural analysis of lignans in Schisandra chinensis rat plasma after oral administration the herbal extract. Six extract identified unambiguously by comparing retention time, their characteristic ultraviolet (UV) absorption accurate measurement. A formula database known was established, against...
Aconite roots are popularly used in herbal medicines China. Many cases of accidental and intentional intoxication with this plant have been reported; some these fatal because the toxicity aconitum is very high. It thus important to detect identify alkaloids biofluids. In work, an improved method employing LC-TOFMS multivariate data analysis was developed for screening major their metabolites rat urine following oral administration aconite extract. Thirty-four signals highlighted by...
Candida albicans, the most common fungal pathogen related to colonization and biofilm formation on surfaces of indwelling medical devices, shows high resistance commonly used antifungal drugs. In this study, an ion-pairing chromatography-porous graphitic carbon column coupled with a time-of-flight mass spectrometry (IP-PGC-TOF/MS) system was developed for targeted untargeted profiling metabolites involved in planktonic growth C. albicans. Using analysis, 16 differential were screened...
Celastrol is well known for its anti-cancer effects, yet specific mechanisms against colon cancer are still not fully elucidated. In this study, cytotoxic effect of celastrol HCT116 cells was investigated based on cell viability assay and flow cytometry assay, the possible mechanism explored using a strategy combining metabolic profiling targeted metabolite analysis ultra performance liquid chromatography (UPLC)/MS. found to inhibit growth induce apoptosis. Metabolomics revealed...
ABSTRACT A liquid chromatography–time‐of‐flight mass spectrometric (LC‐TOFMS) method has been developed for analysis of components in Qifu decoction (QFD), a traditional Chinese medical formula consisting Radix Astragali and Acontium carmichaeli , rat plasma after oral administration. Based on accurate measurements within 3 ppm error each molecular ion subsequent fragment ions TOFMS, as well matching empirical formulae with those published the in‐house chemical library, total 44 major...
Abstract A simple, sensitive and rapid method for analysis of six lignans in rat plasma after oral administration Schisandra chinensis extracts, utilizing liquid chromatography tandem mass spectrometry (LC‐MS), was established validated. Plasma samples were prepared by one‐step protein precipitation using acetonitrile the analytes separated on an SB‐C 18 column (100 mm × 3.0 mm, 3.5 µm) with mobile phase acetonitrile–water at a flow‐rate 0.8 mL/min. Analytes determined single‐quadrupole...