A5103030742- Plant biochemistry and biosynthesis
- Plant Gene Expression Analysis
- Microbial Metabolic Engineering and Bioproduction
- Biochemical and biochemical processes
- Plant-derived Lignans Synthesis and Bioactivity
- Plant Reproductive Biology
- Microbial Natural Products and Biosynthesis
- Phytochemistry and Biological Activities
- Plant tissue culture and regeneration
- Plant Molecular Biology Research
- Toxin Mechanisms and Immunotoxins
- Hops Chemistry and Applications
- Natural product bioactivities and synthesis
- Phytochemical compounds biological activities
- Insect and Pesticide Research
- Heat shock proteins research
- Biochemical Acid Research Studies
- Agriculture and Biological Studies
- Ginseng Biological Effects and Applications
- Plant pathogens and resistance mechanisms
- Food Quality and Safety Studies
- Powdery Mildew Fungal Diseases
- Chemical and Physical Properties in Aqueous Solutions
- Andrographolide Research and Applications
- Polysaccharides and Plant Cell Walls
Second Military Medical University
2015-2025
Dalian Institute of Chemical Physics
2020-2024
Chinese Academy of Sciences
2020-2024
University of California, Davis
2018
Tianjin Medical University Cancer Institute and Hospital
2015
Abstract The flavonoid xanthohumol is an important flavor substance in the brewing industry that has a wide variety of bioactivities. However, its unstable structure results low content beer. Microbial biosynthesis considered sustainable and economically viable alternative. Here, we harness yeast Saccharomyces cerevisiae for de novo from glucose by balancing three parallel biosynthetic pathways, prenyltransferase engineering, enhancing precursor supply, constructing enzyme fusion,...
Lignans, such as lariciresinol and its derivatives, have been identified effective antiviral ingredients in Isatis indigotica. Evidence suggests that the APETALA2/ethylene response factor (AP2/ERF) family might be related to biosynthesis of lignans I. However, special role played by AP2/ERF metabolism underlying putative mechanism still need elucidated. One novel gene, named Ii049, was isolated characterized from indigotica this study. The quantitative real-time PCR analysis revealed Ii049...
Isatis indigotica is a widely used herb for the clinical treatment of colds, fever, and influenza in Traditional Chinese Medicine (TCM). Various structural classes compounds have been identified as effective ingredients. However, little known at genetics level about these active metabolites. In present study, we performed de novo transcriptome sequencing first time to produce comprehensive dataset I. indigotica. A database 36,367 unigenes (average length = 1,115.67 bases) was generated by...
Fifteen genes were predicted to be closely related safranal and crocin production by multi-omic analysis in which CsALDH3 was validated used construct crocetin-producing yeast.
Root and leaf tissue of Isatis indigotica shows notable anti-viral efficacy, are widely used as "Banlangen" "Daqingye" in traditional Chinese medicine. The plants' pharmacological activity is attributed to phenylpropanoids, especially a group lignan metabolites. However, the biosynthesis lignans I. remains opaque. This study describes discovery analysis biosynthetic genes AP2/ERF-type transcription factors involved indigotica. MeJA treatment revealed differential expression three...
Diterpenoids constitute a diverse class of metabolites with critical functions in plant development, defense, and ecological adaptation. Major monocot crops, such as maize (Zea mays) rice (Oryza sativa), deploy blends specialized diterpenoids core components biotic abiotic stress resilience. Here, we describe the genome-wide identification functional characterization stress-related diterpene synthases (diTPSs) dedicated bioenergy crop switchgrass (Panicum virgatum). Mining allotetraploid...
Summary MicroRNAs (miRNAs) play crucial roles in plant development and secondary metabolism through different modes of sequence‐specific interaction with their targets. Artemisinin biosynthesis is extensively regulated by phytohormones. However, the function phytohormone‐responsive miRNAs artemisinin remains enigmatic. Thus, we combined analysis transcriptomics, small RNAs, degradome to generate a comprehensive resource for identifying key miRNA‐target circuits involved phytohormone‐induced...
A lignan, lariciresinol, is an important efficacious compound for the antiviral effect of Isatis indigotica, a widely used herb treatment colds, fever, and influenza. Although some rate-limiting steps lariciresinol biosynthetic pathway are well known, specific roles gene family members in I. indigotica regulating production poorly understood. In present study, correlation analysis between RNA sequencing (RNA-Seq) expression profile lignan content by using hairy roots treated with methyl...
Abstract Phellinus baumii , also called “Sang Huang” in China, is broadly used as a kind of health food or folk medicine Asia for its high biological activities, e.g. anti-tumor, anti-oxidation and anti-inflammatory activities. Although some previous studies have indicated that polysaccharides flavonoids showed the activity inhibiting tumor cells, active metabolites P. needs further research. In our study, stable mutant (A67), generated by ARTP mutagenesis strategy, more significantly cells...
Plants synthesize diverse diterpenoids with numerous functions in organ development and stress resistance. However, the role of glandular trichome (GT) GT-localized biosynthesis plants remains unknown. Here, identification 10 diterpene synthases (diTPSs) revealed diversity diterpenoid Artemisia annua. Protein-protein interactions (PPIs) between AaKSL1 AaCPS2 plastids highlighted their potential modulating metabolic flux to gibberellins (GAs) or ent-isopimara-7,15-diene-derived metabolites...
Hepatocellular carcinoma (HCC) is a leading cause of cancer-related deaths. Novel serum biomarkers are required to increase the sensitivity and specificity screening for early HCC diagnosis. This study employed quantitative proteomic strategy analyze differential expression glycoproteins between normal control samples.Lectin affinity chromatography (LAC) was used enrich from samples. Quantitative mass spectrometric analysis combined with stable isotope dimethyl labeling 2D liquid (LC)...
Background Erigeron breviscapus, a well-known traditional Chinese medicinal herb, is broadly used in the treatment of cerebrovascular disease. Scutellarin, kind flavonoids, considered as material base pharmaceutical activities E. breviscapus. The stable and high content scutellarin critical for quality efficiency breviscapus clinical use. Therefore, understanding molecular mechanism biosynthesis crucial metabolic engineering to increase active compound. However, there virtually no study...
Lignans are a powerful weapon for plants to resist stresses and have diverse bioactive functions protect human health. Elucidating the mechanisms of stereoselective biosynthesis response lignans is important guidance plant improvement. Here, we identified complete pathway stereoselectively synthesize antiviral (–)-lariciresinol glucosides in Isatis indigotica roots, which consists three-step sequential enzymes DIR1/2, PLR, UGT71B2. DIR1 was further as key gene able trigger stress defenses by...
Scutellarin, a flavonoid 7-O-glucuronide, is an essential bioactive compound of Erigeron breviscapus (Vaniot) Hand.-Mazz. used for the treatment cerebrovascular diseases. However, due to overexploitation and overuse, facing problems extinction habitat degradation. In this study, correlation analysis between transcript metabolite profiles MeJA-treated E. at different time points indicated that chalcone isomerase (EbCHI) was primary contributor scutellarin accumulation during biosynthesis....
Abstract The flavonoid xanthohumol is an important flavor substance in the brewing industry that has a wide variety of bioactivities. However, its unstable structure easily oxidized during process, resulting low content beer. Moreover, extraction and purification from plants requires laborious expensive procedures. Microbial biosynthesis considered sustainable economically viable alternative to supply natural products. complex structures products regulation biosynthetic pathways make it...