A5009951838- Plant biochemistry and biosynthesis
- Microbial Natural Products and Biosynthesis
- CRISPR and Genetic Engineering
- Plant Gene Expression Analysis
- Insect Resistance and Genetics
- Plant Molecular Biology Research
- Pharmacological Effects of Natural Compounds
- Plant Reproductive Biology
- Plant tissue culture and regeneration
- Plant Stress Responses and Tolerance
- Insect-Plant Interactions and Control
- Plant Parasitism and Resistance
- Transgenic Plants and Applications
- Malaria Research and Control
- Plant and animal studies
- Neurobiology and Insect Physiology Research
- Bioactive natural compounds
- Lipid metabolism and biosynthesis
- Natural product bioactivities and synthesis
- Plant-derived Lignans Synthesis and Bioactivity
- Leaf Properties and Growth Measurement
- Biological Activity of Diterpenoids and Biflavonoids
- RNA modifications and cancer
- Photosynthetic Processes and Mechanisms
- Calcium signaling and nucleotide metabolism
Shanghai University of Traditional Chinese Medicine
2019-2024
Zero to Three
2020-2024
State Administration of Traditional Chinese Medicine of the People's Republic of China
2023
Second Military Medical University
2017-2022
Health First
2022
Shanghai Jiao Tong University
2012-2021
Summary Six transcription factors of APETALA2/ethylene‐response factor ( AP 2/ ERF ) family were cloned and analyzed in A rtemisia annua . Real‐time quantitative polymerase chain reaction RT ‐ Q PCR showed that Aa ORA exhibited similar expression patterns to those amorpha‐4,11‐diene synthase gene ADS ), cytochrome P450‐dependent hydroxylase CYP71AV1 double bond reductase 2 DBR2 different tissues a is trichome‐specific factor, which expressed both glandular secretory trichomes GST s)...
The plant Artemisia annua is well known due to the production of artemisinin, a sesquiterpene lactone that widely used in malaria treatment. Phytohormones play important roles secondary metabolism, such as jasmonic acid (JA), which can induce artemisinin biosynthesis A. annua. Nevertheless, JA-inducing mechanism remains poorly understood. expression gene AaMYC2 was rapidly induced by JA and binds G-box-like motifs within promoters CYP71AV1 DBR2, are key structural genes biosynthetic pathway....
Glandular trichomes are generally considered biofactories that produce valuable chemicals. Increasing glandular trichome density is a very suitable way to improve the productivity of these metabolites, but little known about regulation formation. Phytohormone jasmonate (JA) promotes initiation in various plants, its mechanism also unknown. By searching transcription factors regulated by JA Artemisia annua, we identified novel homeodomain-leucine zipper factor, HOMEODOMAIN PROTEIN 1 (AaHD1),...
Summary Artemisinin is a type of sesquiterpene lactone well known as an antimalarial drug, and specifically produced in glandular trichomes Artemisia annua . However, the regulatory network for artemisinin biosynthetic pathway remains poorly understood. Exploration trichome‐specific transcription factors would facilitate elucidation mechanism biosynthesis. The WRKY factor GLANDULAR TRICHOME ‐ SPECIFIC 1 ( Aa GSW ) was cloned analysed A. exhibited similar expression patterns to genes AP 2/...
Summary The glandular secretory trichomes ( GST s) on Artemisia annua leaves have the capacity to secrete and store artemisinin, a compound which is most effective treatment for uncomplicated malaria. An strategy improve artemisinin content therefore increase density of s in A. . However, formation mechanism remains poorly understood. To explore mechanisms initiation , we screened myeloblastosis MYB ) transcription factor genes from transcriptome database identified MIXTA factor, Aa 1,...
The NAC (NAM, ATAF and CUC) superfamily is one of the largest plant-specific transcription factor families. factors always play important roles in response to various abiotic stresses. A gene AaNAC1 containing a complete open reading frame (ORF) 864 bp was cloned from Artemisia annua. expression could be induced by dehydration, cold, salicylic acid (SA) methyl jasmonate (MJ), suggesting that it might key regulator stress signaling pathways A. shown localized nuclei transforming tobacco leaf...
Drought is one of the most severe abiotic factors restricting plant growth and yield. Numerous genes functioning in drought response are regulated by abscisic acid (ABA) dependent independent pathways, but knowledge interplay between two pathways still limited. Here, we integrated transcriptome sequencing network analyses to explore interplays ABA-dependent ABA-independent responding stress Arabidopsis thaliana. We identified 211 differentially expressed (DEGs) 1,118 DEGs under stress....
Summary Artemisinin, a sesquiterpene lactone widely used in malaria treatment, was discovered the medicinal plant Artemisia annua . The biosynthesis of artemisinin is efficiently regulated by jasmonate (JA) and abscisic acid (ABA) via regulatory factors. However, mechanisms linking JA ABA signalling with through an associated network downstream transcription factors (TFs) remain enigmatic. Here we report AaTCP15, dual‐responsive teosinte branched1/cycloidea/proliferating (TCP) TF, which...
The important antimalarial drug artemisinin is biosynthesized and stored in Artemisia annua glandular trichomes the content correlates with trichome density; however, factors affecting development are largely unknown. Here, we demonstrate that A. R2R3 MYB transcription factor TrichomeLess Regulator 1 (TLR1) negatively regulates development. In annua, TLR1 overexpression lines had 44.7%-64.0% lower density 11.5%-49.4% contents TLR1-RNAi 33%-93.3% higher 32.2%-84.0% compared non-transgenic...
Lignans, such as lariciresinol and its derivatives, have been identified effective antiviral ingredients in Isatis indigotica. Evidence suggests that the APETALA2/ethylene response factor (AP2/ERF) family might be related to biosynthesis of lignans I. However, special role played by AP2/ERF metabolism underlying putative mechanism still need elucidated. One novel gene, named Ii049, was isolated characterized from indigotica this study. The quantitative real-time PCR analysis revealed Ii049...
There are many biosynthetic pathways competing for the metabolic flux with artemisinin pathway in Artemisia annua L. To study relationship between genes encoding enzymes at branching points and pathway, β-caryophyllene, β-farnesene squalene were sprayed on young seedlings of A. . Transient expression assays indicated that transcription levels β-caryophyllene synthase ( CPS ), BFS ) SQS inhibited by squalene, respectively, while some increased. Thus, inhibition these may be helpful to improve...
Artemisinin is a sesquiterpene lactone endoperoxide extracted from traditional Chinese medicinal plant Artemisia annua. Artemisinin-based combination therapies (ACTs) are recommended as the best treatment of malaria by World Health Organization (WHO). Both phytohormone jasmonic acid (JA) and light promote artemisinin biosynthesis in A. Interestingly, we found that increase JA was dependent on light. However, relationship between two signal pathways mediated remains unclear. Here, collected...
Abstract Artemisinin is a sesquiterpene lactone produced by the Chinese traditional herb Artemisia annua and used for treatment of malaria. It known that salicylic acid (SA) can enhance artemisinin content but mechanism which it does so not known. In this study, we systematically investigated basic leucine zipper family transcription factor, AaTGA6, involved in SA signaling to regulate biosynthesis. We found specific vivo vitro binding AaTGA6 protein ‘TGACG’ element AaERF1 promoter....
Until recently, many studies on the role of phytohormones in plant secondary metabolism focused jasmonic acid (JA), salicylic (SA), gibberellins (GA), and abscisic (ABA). It is now clear that phytohormone-induced regulation signaling occurs via biosynthetic pathway genes at transcriptional level or through posttranslational regulation, an increase metabolite deposition (e.g., trichomes). Here, we summarize recent advances, updating current reports molecular machinery JA, SA, GA, ABA involved...
Artemisinin is an effective component of drugs against malaria. The regulation artemisinin biosynthesis at the forefront research. Previous studies showed that AaWRKY1 can regulate expression ADS, which first key enzyme in biosynthetic pathway. In this study, was cloned, and it activated ADSpro CYPpro tobacco using dual-LUC assay. To further study function AaWRKY1, pCAMBIA2300-AaWRKY1 construct under 35S promoter generated. Transgenic plants containing were obtained, four independent lines...
The phytohormone abscisic acid (ABA) plays an important role in plant development and environmental stress response. In this study, we cloned ABA receptor orthologue, AaPYL9, from Artemisia annua L. AaPYL9 is expressed highly leaf flower. protein can be localized both nucleus cytoplasm. Yeast two-hybrid assay shows specifically interact with AtABI1 but not AtABI2, AtHAB1 or AtHAB2. enhance the interaction between while AaPYL9-89 Pro→Ser AaPYL9-116 His→Ala point mutations abolishes...
Summary MicroRNAs (miRNAs) play crucial roles in plant development and secondary metabolism through different modes of sequence‐specific interaction with their targets. Artemisinin biosynthesis is extensively regulated by phytohormones. However, the function phytohormone‐responsive miRNAs artemisinin remains enigmatic. Thus, we combined analysis transcriptomics, small RNAs, degradome to generate a comprehensive resource for identifying key miRNA‐target circuits involved phytohormone‐induced...
Abstract Artemisinin is primarily synthesized and stored in the subepidermal space of glandular trichomes Artemisia annua . The augmentation trichome density has been demonstrated to enhance artemisinin yield. However, existing literature lacks insights into correlation between stratum corneum trichomes. This study aims unravel involvement TrichomeLess Regulator 3 ( TLR3 ), which encodes transcription factor, biosynthesis its potential association with corneum. was identified as a candidate...