A5058539150- Melanoma and MAPK Pathways
- Cancer Immunotherapy and Biomarkers
- Prostate Cancer Treatment and Research
- Immunotherapy and Immune Responses
- Cancer-related molecular mechanisms research
- IL-33, ST2, and ILC Pathways
- interferon and immune responses
- Cytokine Signaling Pathways and Interactions
- Estrogen and related hormone effects
- NF-κB Signaling Pathways
- S100 Proteins and Annexins
- Immune cells in cancer
- RNA modifications and cancer
- Sperm and Testicular Function
- Circular RNAs in diseases
- Eosinophilic Esophagitis
- RNA Interference and Gene Delivery
- Psoriasis: Treatment and Pathogenesis
- MicroRNA in disease regulation
- Ubiquitin and proteasome pathways
- Immune Cell Function and Interaction
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA regulation and disease
- Cancer Research and Treatments
- Molecular Biology Techniques and Applications
Second Affiliated Hospital of Nanjing Medical University
2020-2024
Tulane University
2015-2024
Louisiana Cancer Research Center
2012-2024
Nanjing Medical University
2020-2024
McCormick (United States)
2008-2023
George Washington University
2007-2023
Sichuan University
2023
Huaiyin Institute of Technology
2023
Boston Medical Center
2021-2022
Boston University
2021-2022
Background Patients who have esophageal eosinophilia without gastroesophageal reflux disease (GERD) nevertheless can respond to proton pump inhibitors (PPIs), which anti-inflammatory actions independent of effects on gastric acid secretion. In cell cultures, omeprazole has been reported inhibit Th2 cytokine-stimulated expression eotaxin-3, an eosinophil chemoattractant contributing in eosinophilic esophagitis (EoE). The objective this study was elucidate molecular mechanisms underlying PPI...
Epigenetic alterations occur in many physiological and pathological processes. N6-methyladenosine (m6A) modification is the most prevalent eukaryotic mRNAs. However, role of m6A angiogenesis remains elusive. In this study, we showed that level was significantly upregulated endothelial cells mouse retinas following hypoxic stress, which caused by increased METTL3 levels. silencing or overexpression altered cell viability, proliferation, migration, tube formation vitro. knockout vivo decreased...
Abstract The symptoms of vaginal candidiasis exacerbate in the second half menstrual cycle premenopausal women when serum estradiol level is elevated. Estradiol has been shown to inhibit Th17 differentiation and production antifungal IL-17 cytokines. However, little known about mechanisms. In present study, we used mouse splenocytes found that inhibited through downregulation Rorγt mRNA protein expression. activated estrogen receptor (ER)α recruit repressor activity (REA) form ERα/REA...
Rationale: Microvascular complication is a major cause of morbidity and mortality among the patients with diabetes.Pericyte dysfunction predominant pathological manifestation microvascular complication.N 6 -methyladenosine (m A) serves as most prevalent modification in eukaryotic mRNAs.However, role m A RNA pericyte still unclear.Methods: Quantitative polymerase chain reactions western blots were conducted to detect change pericytes mouse retinas following diabetic stress.MTT assay,...
Posttraumatic stress disorder (PTSD) is associated with decreased activity in the dorsolateral prefrontal cortex (DLPFC), brain region that regulates working memory and preparation selection of fear responses.We investigated gene expression profiles DLPFC Brodmann area (BA) 46 postmortem patients (n=6) without PTSD using human mitochondria-focused cDNA microarrays.Our study revealed PTSD-specific fingerprints 800 informative genes across all these 12 BA46 samples, 119 (±>1.25,p<0.05) 42...
Abstract The contributions of interleukin (IL)-17 to cancer remain unclear and somewhat controversial. We took a genetic approach explore its role in prostate cancers by interbreeding IL-17 receptor C (IL-17RC)–deficient mice with that are conditionally mutant for PTEN, one established preclinical model cancer. Mice were IL-17RC–deficient (IL-17RC−) displayed prostates smaller than maintained IL-17RC expression (IL-17RC+). In addition, IL-17RC− developed reduced number invasive...
Interleukin-17 (IL-17) is a proinflammatory cytokine that plays important roles in inflammation, autoimmunity, and cancer. The purpose of this study was to determine if IL-17 indirectly regulates macrophage differentiation through up-regulation cyclooxygenase-2 (COX-2) expression the cancer cell lines.Human cervical HeLa, human lung A549, mouse prostate Myc-CaP/CR lines were treated with recombinant IL-17; Western blot analysis, enzyme-linked immunosorbent assay, quantitative real-time...
Endometrial cancer (EC) is the most frequent gynecological malignancy and a major cause of morbidity mortality for women worldwide. Programmed cell death protein 1 (PD‑1) its ligands programmed ligand (PD‑L1) 2 (PD‑L2) have been well studied in lung cancer, melanoma renal‑cell cancer. However, few studies performed EC. The purpose present study was to assess expression PD‑1, PD‑L1 PD‑L2 35 human normal endometrial tissue samples 75 EC using immunohistochemical staining. It found that 61.3%...
Although most studies on treatments for eosinophilic esophagitis (EoE) have focused effects in the epithelium, EoE is a transmural disease. Eosinophils that infiltrate subepithelial layers of esophagus lead to fibrosis and serious complications EoE, current therapies shown minimal this fibrosis. We aimed elucidate T helper (Th)2 cytokine esophageal fibroblasts explore potential fibroblast-targeted EoE.We established telomerase-immortalized from human biopsies. stimulated these with Th2...
Inflammation-mediated crosstalk between neuroglial cells and endothelial (ECs) is a fundamental feature of many vascular diseases. Nevertheless, the landscape inflammatory processes during diabetes-induced microvascular dysfunction remains elusive. Here, we applied single-cell RNA sequencing to elucidate transcriptional diabetic retinopathy (DR). The transcriptome characteristics microglia ECs revealed two microglial subpopulations three EC populations. Exploration intercellular showed that...
Human malignant melanoma has poor prognosis because of resistance to apoptosis and therapy. We describe identification the expression profile 1,037 mitochondria-focused genes 84 survival-apoptosis in 21 cell lines 3 normal melanocyte controls using recently developed hMitChip3 cDNA microarrays. Unsupervised hierarchical clustering analysis informative genes, classified these into type A (n = 12) B 9). Three hundred fifty-five (34.2%) displayed significant (P ≤ 0.030; false discovery rate...
BACKGROUND Chronic inflammation has been associated with the development and progression of human cancers including prostate cancer. The exact role inflammatory Th17‐IL‐17 pathway in cancer remains unknown. In this study, we aimed to determine importance Th17 cells IL‐17 a Pten ‐null mouse model. METHODS mice were treated by inhibitor SR1001 or anti‐mouse monoclonal antibody from 6 weeks age up 12 age. For treatment, injected intraperitoneally (i.p.) twice day vehicle SR1001, which was...
BACKGROUND Interleukin‐17 (IL‐17) has been demonstrated to promote formation and growth of hormone‐naïve prostate adenocarcinoma in mice. IL‐17's role development castration‐resistant cancer is unknown. In the present study, we investigated a mouse model. METHODS IL‐17 receptor C (IL‐17RC) deficient mice were interbred with Pten conditional mutant produce RC + that maintained IL‐17RC expression − deficient. Male ‐null castrated at 16 weeks age when invasive had already formed. At 30 age, all...
Abstract Obesity is a chronic inflammation with increased serum levels of insulin, insulin‐like growth factor 1 ( IGF 1), and interleukin‐17 IL ‐17). The objective this study was to test hypothesis that insulin enhance ‐17‐induced expression inflammatory chemokines/cytokines through glycogen synthase kinase 3β GSK 3B)‐dependent mechanism, which can be inhibited by melatonin. We found insulin/ lithium chloride enhanced C‐X‐C motif ligand (Cxcl1) C‐C 20 (Ccl20) in the Gsk3b +/+ , but not −/−...
The role of Th17 cells in prostate cancer (PCa) is not fully understood. transcription factor BATF controls the differentiation cells. Mice deficient Batf do produce
BACKGROUND Extravasation is a critical step in cancer metastasis, which adhesion of intravascular cells to the vascular endothelial controlled by cell surface molecules. The role interleukin‐17 (IL‐17), insulin, and insulin‐like growth factor 1 (IGF1) prostate unknown, subject present study. METHODS Human umbilical vein (HUVECs) human lines (PC‐3, DU‐145, LNCaP, C4–2B) were analyzed for expression molecule (VCAM‐1), integrins, cluster differentiation 44 (CD44) using flow cytometry Western...
Interleukin-17 (IL-17) plays important roles in inflammation, autoimmune diseases, and some cancers. Obese people are a chronic inflammatory state with increased serum levels of IL-17, insulin, insulin-like growth factor 1 (IGF1). How these factors contribute to the status that promotes development aggressive prostate cancer obese men is largely unknown. We found that, mice, hyperinsulinemia enhanced IL-17-induced expression downstream proinflammatory genes IL-17 receptor A (IL-17RA),...
Endothelial tip cell specialization plays an essential role in angiogenesis, which is tightly regulated by the complicated gene regulatory network. Circular RNA (circRNA) a type of covalently closed non-coding that regulates expression eukaryotes. Here, we report levels circMET are significantly upregulated retinas mice with oxygen-induced retinopathy, choroidal neovascularization, and diabetic retinopathy. silencing reduces pathological angiogenesis inhibits vivo. also decreases endothelial...