A5023879401- Plant biochemistry and biosynthesis
- CRISPR and Genetic Engineering
- Transgenic Plants and Applications
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Plant Molecular Biology Research
- Plant Gene Expression Analysis
- RNA and protein synthesis mechanisms
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Insect-Plant Interactions and Control
- Arsenic contamination and mitigation
- Pharmacological Effects of Natural Compounds
- Essential Oils and Antimicrobial Activity
- Genomics, phytochemicals, and oxidative stress
- Plant Physiology and Cultivation Studies
- Viral Infectious Diseases and Gene Expression in Insects
- Toxin Mechanisms and Immunotoxins
- Pharmacogenetics and Drug Metabolism
- Plant responses to elevated CO2
- Postharvest Quality and Shelf Life Management
- Phytochemical compounds biological activities
- Microbial Natural Products and Biosynthesis
- Insect and Pesticide Research
Shanghai Jiao Tong University
2021-2024
Wenzhou Medical University
2022
Shandong University
2017-2019
Glandular secretory trichomes (GSTs) can secrete and store a variety of specific metabolites. By increasing GST density, valuable metabolites be enhanced in terms productivity. However, the comprehensive detailed regulatory network initiation still needs further investigation. screening complementary DNA library derived from young leaves Artemisia annua, we identified MADS-box transcription factor, AaSEPALLATA1 (AaSEP1), that positively regulates initiation. Overexpression AaSEP1 A. annua...
Artemisia annua, a traditional Chinese medicinal plant, remains the only plant source for artemisinin production, yet few genes have been identified to be involved in both response biotic stresses, such as pathogens, and biosynthesis. Here, we isolated WRKY transcription factor (TF) AaWRKY17, which could significantly increase content resistance Pseudomonas syringae A. annua. Yeast one-hybrid (Y1H), dual-luciferase (dual-LUC), electrophoretic mobility shift assay (EMSA) results showed that...
The Agrobacterium-mediated transient transformation, which proved effective in diverse plant species, has been widely applied for high-throughput gene function studies due to its simplicity, rapidity, and high efficiency. Despite the efforts have made on Artemisia annua expression, achieving functional characterization basing a fast easy-manipulated transformation system A. remains challenging.The first pair of true leaves is an ideal candidate Agrobacterium injection. EHA105 was optimal...
The plant Artemisia annua L. is famous for producing "artemisinin", which an essential component in the treatment of malaria. glandular secretory trichomes (GSTs) on leaves A. secrete and store artemisinin. Previous research has demonstrated that raising GST density can effectively raise artemisinin content. However, molecular mechanism initiation not fully understood yet. In this study, we identified MYB transcription factor, AaMYB108-like, co-induced by light jasmonic acid, positively...
Plant natural products (PNPs) are active substances indispensable to human health with a wide range of medical and commercial applications. However, excessive population growth, overexploitation resources, expensive total chemical synthesis have led recurrent supply shortages. Despite the fact that microbial production platform solved these challenges, still has drawbacks such as environmental pollution, high costs, non-green production. In this study, an efficient for PNPs based on...
Abstract Mosses have adapted to the Antarctic environment and are an ideal medium for studying plant resistance abiotic stress. Chalcone synthase is first committed enzyme in flavonoid metabolic pathway, which plays indispensable role adversity. In this study, six genes ( Pn021, PnCHS088, Pn270, PnCHS444, PnCHS768 Pn847 ) were identified moss Pohlia nutans Lindberg transcriptome by reverse transcription polymerase chain reaction (RT-PCR) rapid amplification of cDNA ends (RACE). Sequence...
The plant Artemisia annua is well known for its production of artemisinin, a sesquiterpene lactone that an effective antimalarial compound. Although remarkable progress has been made toward understanding artemisinin biosynthesis, the effect MADS-box family transcription factors on biosynthesis still poorly understood. In this study, we identified MADS factor, AaSEP4, was predominantly expressed in trichome. AaSEP4 acts as nuclear-localized transcriptional activator activating expression...
Abstract The Agrobacterium-mediated transient expression system has been developed and applied to various plants as an alternative stable transformation. However, its application in tomatoes is still limited due low efficiency. In this study, we describe improved vacuum-infiltration that can be used both tomato fruits leaves. Notably, study the first report of vacuum infiltration attached fruits. feasibility Micro-Tom was confirmed by assays, including multiple fluorescent protein analysis,...
Background Environmental substances such as pesticides are well-known in link with Parkinson’s disease (PD) risk. Enzymes including cytochromes P450 (CYPs), esterases and glutathione S-transferases (GSTs) responsible for the xenobiotic metabolism may functionally compensate each other subtypes same class. We hypothesize that genetic effects of class modulate PD risk stronger a synergistic way than individually. Methods selected 14 polymorphic loci out 13 genes which encode enzymes classes...
Abstract Medicinal plants with high-value pharmaceutical ingredients have attracted research attention due to their beneficial effects on human health. Cell wall-free protoplasts of can be used evaluate the efficiency genome editing mutagenesis. The capabilities gene in medicinal remain fully explored owing complex genetic background and shortfall suitable transformation. Here, we took Salvia miltiorrhiza as a representative example for developing method screen favorable elements high...
Trichomes, which are classified as glandular or non-glandular, hair-like epidermal structures that present on aerial parts of most plant species. Glandular secretory trichomes (GSTs) have the capacity to secrete and store specialized metabolites, widely used natural pesticides, food additives, fragrance ingredients pharmaceuticals. Isolating individual is an essential way for identifying trichome-specific gene functions discovering novel metabolites. However, isolation difficult...