A5100345373- RNA modifications and cancer
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Ferroptosis and cancer prognosis
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Cancer-related gene regulation
- RNA Research and Splicing
- Autophagy in Disease and Therapy
- RNA and protein synthesis mechanisms
- Epigenetics and DNA Methylation
- Mitochondrial Function and Pathology
- Cancer Mechanisms and Therapy
- Cancer, Lipids, and Metabolism
- RNA Interference and Gene Delivery
- Endoplasmic Reticulum Stress and Disease
- Cell death mechanisms and regulation
- Cancer-related Molecular Pathways
- Peptidase Inhibition and Analysis
- Cancer Research and Treatments
- Heat shock proteins research
- DNA Repair Mechanisms
- Metabolomics and Mass Spectrometry Studies
- Cancer therapeutics and mechanisms
Bengbu Medical College
2020-2025
Dalian Medical University
2015-2025
First Affiliated Hospital of Dalian Medical University
2024-2025
First Affiliated Hospital of Kunming Medical University
2013-2025
Kunming Medical University
2013-2025
Tianjin Medical University General Hospital
2009-2025
University of Wisconsin–Madison
2025
University of Wisconsin Carbone Cancer Center
2025
Taizhou Fourth People's Hospital
2024-2025
Jinan University
2014-2025
Mitochondrial morphology is dynamically remodeled by fusion and fission in cells, dysregulation of this process closely implicated tumorigenesis. However, the mechanism which mitochondrial dynamics influence cancer cell survival considerably less clear, especially hepatocellular carcinoma (HCC). In study, we systematically investigated alteration its functional role regulation autophagy HCC survival. Furthermore, underlying molecular mechanisms therapeutic application were explored depth....
A switch from autophagy to apoptosis is implicated in chondrocytes during the osteoarthritis (OA) progression with currently unknown mechanism(s). In this study we utilized a flow fluid shear stress (FFSS) model cultured and unilateral anterior crossbite (UAC) animal model. We found that both FFSS UAC actively induced endoplasmic reticulum (ERS) temporomandibular joints (TMJ) chondrocytes, as demonstrated by dramatic increases expression of HSPA5, p-EIF2AK3, p-ERN1 ATF6. Interestingly,...
Lipid overload-induced heart dysfunction is characterized by cardiomyocyte death, myocardial remodeling, and compromised contractility, but the impact of excessive lipid supply on cardiac function remains poorly understood.To investigate regulation mitochondrial fission protein Drp1 (dynamin-related 1) in death dysfunction.Mice fed a high-fat diet (HFD) developed signs obesity type II diabetes mellitus, including hyperlipidemia, hyperglycemia, hyperinsulinemia, hypertension. HFD for 18 weeks...
Significant embryo loss remains a serious problem in pig production. Reactive oxygen species (ROS) play critical role embryonic implantation and placentation. However, the potential mechanism of ROS on porcine trophectoderm (pTr) cell fate during peri-implantation period has not been investigated. This study aimed to elucidate effects pTr phenotypes regulatory attachment differentiation. Herein, results showed that exogenous H 2 O inhibited viability, arrested cycle at S G2/M phases,...
It has been a long debate whether the 98% 'non-coding' fraction of human genome can encode functional proteins besides short peptides. With full-length translating mRNA sequencing and ribosome profiling, we found that up to 3330 non-coding RNAs (lncRNAs) were bound ribosomes with active translation elongation. shotgun proteomics, 308 lncRNA-encoded new detected. A total 207 unique peptides these verified by multiple reaction monitoring (MRM) and/or parallel (PRM); 10 immunoblotting. We...
Abstract Background Quercetin, a natural flavonoid compound, is potent cancer therapeutic agent widely found in fruit and vegetables. It has been reported to induce growth inhibition apoptosis both A549 H1299 human lung cells. However, the effect of quercetin‐induced autophagy on possible mechanism cells have not yet critically examined. Methods were treated with different concentrations quercetin for 24 hours. Cell was measured by cell counting kit‐8 (CCK‐8) assay, whereas assessed western...
Immune checkpoint inhibitors have greatly improved the prognoses of diverse advanced malignancies, including gastric and gastroesophageal junction (G/GEJ) cancer. However, role anti-programmed cell death protein-1 treatment in neoadjuvant setting remains unclear. This phase 2 study aimed to evaluate sintilimab plus CapeOx as a regimen patients with resectable G/GEJ adenocarcinoma. Eligible adenocarcinoma stage cT3-4NanyM0 were enrolled. Patients received (3 mg/kg for cases <60 kg or 200...
Abstract Transfer RNA (tRNA)-derived small RNAs (tsRNAs) are a new type of non-coding (ncRNAs) produced by the specific cleavage precursor or mature tRNAs. tsRNAs involved in various basic biological processes such as epigenetic, transcriptional, post-transcriptional, and translation regulation, thereby affecting occurrence development human diseases, including cancers. Recent studies have shown that play an important role tumorigenesis regulating behaviors malignant proliferation, invasion...
Generally, mRNAs that prematurely terminate translation are abnormally low in abundance. In the case of mammalian cells, nonsense codons most often mediate a reduction abundance newly synthesized, nucleus-associated mRNA by mechanism is not well understood. With aim defining cis-acting sequences important to process, effects particular β-globin gene rearrangements on metabolism harboring one series have been assessed. Results indicate located 54 bp or more upstream 3′-most intron, intron 2,...
Cell-secreted miRNAs are highly stable and can serve as biomarkers for various diseases signaling molecules in intercellular communication. The mechanism underlying the stability of circulating miRNAs, however, remains incompletely understood. Here we show that Argonaute 2 (Ago2) complexes microvesicles (MVs) provide specific non-specific protection miRNA cell-secreted MVs, respectively. First, resistance MV-encapsulated to RNaseA was both depended on intact vesicular structure MVs...
Genome-wide microRNA (miRNA) profiling of 82 glioblastomas demonstrated that miR-181d was inversely associated with patient overall survival after correcting for age, Karnofsky performance status, extent resection, and temozolomide (TMZ) treatment. This association validated using the Cancer Genome Atlas (TCGA) dataset (n= 424) an independent cohort 35). In these cohorts, evident in patients who underwent TMZ treatment but not observed without therapy. Bioinformatic analysis potential genes...
We have shown previously that wounding of human corneal epithelial (HCE) cells resulted in epidermal growth factor receptor (EGFR) transactivation through ectodomain shedding heparin-binding EGF-like (HB-EGF). However, the initial signal to trigger these signaling events response cell injury remains elusive. In present study, we investigated role ATP released from injured EGFR HCE as well BEAS 2B cells, a bronchial line. Wounding monolayer release into culture medium. The wound-induced rapid...
Abstract The mammalian nicotinamide‐adenine dinucleotide (NAD)‐dependent deacetylase Sirt1 impacts different processes involved in the maintenance of brain integrity and pathogenic pathways associated with several neurodegenerative disorders, including Alzheimer's disease. Here we used human transgenic mice to demonstrate that neuron‐specific overexpression promoted neurite outgrowth improved cell viability under normal nutrient‐limiting conditions primary culture systems...
AbstractIn our study, we detect the levels of three micro-RNAs (miRNAs; miR-21, miR-133a and miR-146a) in plasma 120 Chinese postmenopausal women who were divided into groups (normal, osteopenia osteoporosis) according to T-scores. Downregulation as well upregulation miR-133a, was validated osteoporosis patients versus normal group. The difference expression regarding miR-146a level among not significant (p > 0.01). circulating miRNA bone mineral density (BMD) examined during a multiple...
Blockade of lysosomal calcium release due to lipid accumulation has been shown inhibit mTORC1 signaling. However, the mechanism by which regulates remained undefined. Herein we report that proper through channel TRPML1 is required for activation. depletion inhibits activity, while overexpression or pharmacologic activation opposite effect. Lysosomal activates inducing association calmodulin (CaM) with mTOR. Blocking interaction between mTOR and CaM antagonists significantly activity....
Circular RNAs (circRNAs) are a new type of non-coding and their functions in gastric cancer (GC) remain unclear. Recent studies have revealed that circRNAs play an important role development certain types pathological responses, acting as microRNA (miRNA) sponges to regulate gene expression. CircNet was used screen potential validated circYAP1 expression levels 17 GC tissues by quantitative real-time PCR (qRT-PCR) another 80 paired FISH. CircYAP1 overexpression knockdown experiments were...
The recently discovered clustered regularly interspaced short palindromic repeat (CRISPR)-mediated virus defense represents an adaptive immune system in many bacteria and archaea. Small CRISPR RNAs cause cleavage of complementary invading nucleic acids conjunction with associated protein or a complex. Here, we show CRISPR-mediated mRNA from the hyperthermophilic archaeon Sulfolobus solfataricus. More than 40% targeted could be cleaved, as demonstrated by quantitative polymerase chain...