A5033377592- Ocular Infections and Treatments
- Ocular Diseases and Behçet’s Syndrome
- Immune Response and Inflammation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Retinal and Optic Conditions
- Mosquito-borne diseases and control
- Antibiotic Resistance in Bacteria
- Retinal Diseases and Treatments
- Antimicrobial Peptides and Activities
- Antifungal resistance and susceptibility
- Bacterial Identification and Susceptibility Testing
- Autophagy in Disease and Therapy
- Bacterial biofilms and quorum sensing
- Corneal Surgery and Treatments
- Glaucoma and retinal disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Antimicrobial Resistance in Staphylococcus
- Viral Infections and Vectors
- Hippo pathway signaling and YAP/TAZ
- Ocular Surface and Contact Lens
- Pharmaceutical and Antibiotic Environmental Impacts
- Herpesvirus Infections and Treatments
- Bacteriophages and microbial interactions
- Sphingolipid Metabolism and Signaling
- Traumatic Ocular and Foreign Body Injuries
Banaras Hindu University
2023-2025
Institute of Medical Sciences
2023-2025
Wayne State University
2015-2024
National Institute of Ayurveda
2024
Kresge Eye Institute
2014-2024
All India Institute of Medical Sciences Bhopal
2022-2024
Vivekananda Global University
2022-2023
Translational Health Science and Technology Institute
2022-2023
Institute of Chartered Financial Analysts of India
2023
National Bureau of Fish Genetic Resources
2022
Purpose: To assess vitreous levels of inflammatory cytokines and neurotrophins (NTs) in diabetic retinopathy (DR) elucidate their potential roles. Methods: A prospective study was performed on 50 samples obtained from patients with DR (n = 22) the nondiabetic controls 28). All were candidates for vitrectomy. Inflammatory cytokine NT determined ELISA. Potential source role NTs by using human retinal Müller glia mouse photoreceptor cells challenging them TNF-α or IL-1β, followed detection cell...
The objective of this study was to examine the expression Toll-like receptor 3 (TLR3) by human corneal epithelial cells (HCECs) and determine whether exposure TLR3 agonist polyinosinic-polycytidylic acid [poly(I:C)] induces an antiviral response in these cells. Fluorescence-activated cell sorter (FACS) analysis revealed be constitutively expressed distributed intracellularly HCECs. Stimulation HCECs with poly(I:C) induced activation nuclear factor (NF)-kappaB production proinflammatory...
Thioredoxin Interacting Protein (TXNIP) mediates retinal inflammation, gliosis, and apoptosis in experimental diabetes. Here, we investigate the temporal response of Muller glia to high glucose (HG) TXNIP expression using a rat cell line (rMC1) culture. We examined if HG-induced evokes host defense mechanisms rMC1 metabolic abnormalities. HG causes sustained up-regulation (2 h 5 days), ROS generation, ATP depletion, ER stress, inflammation. Various cellular are activated by HG: (i) NLRP3...
We have shown previously that wounding of human corneal epithelial (HCE) cells resulted in epidermal growth factor receptor (EGFR) transactivation through ectodomain shedding heparin-binding EGF-like (HB-EGF). However, the initial signal to trigger these signaling events response cell injury remains elusive. In present study, we investigated role ATP released from injured EGFR HCE as well BEAS 2B cells, a bronchial line. Wounding monolayer release into culture medium. The wound-induced rapid...
Zika virus (ZIKV) is an important pathogen that causes not only neurologic, but also ocular, abnormalities. Thus, it imperative models to study ZIKV pathogenesis in the eye are developed identify potential targets for interventions. Here, we studied interactions with human retinal cells and evaluated ZIKV's pathobiology mouse eyes. We showed lining blood-retinal barrier (BRB), endothelium, pigment epithelium (RPE) were highly permissive susceptible ZIKV-induced cell death. Direct inoculation...
Myopia is the most common vision disorder and leading cause of visual impairment worldwide. However, gene variants identified to date explain less than 10% variance in refractive error, leaving majority heritability unexplained ("missing heritability"). Previously, we reported that expression APLP2 was strongly associated with myopia a primate model. Here, found low-frequency near 5'-end were error prospective UK birth cohort (n = 3,819 children; top SNP rs188663068, p 5.0 × 10−4) CREAM...
Abstract Viruses are known to perturb host cellular metabolism enable their replication and spread. However, little is about the interactions between Zika virus (ZIKV) infection metabolism. Using primary human retinal vascular endothelial cells an established cell line, we investigated role of AMP-activated protein kinase (AMPK), a master regulator energy metabolism, in response ZIKV challenge. caused time-dependent reduction active phosphorylated state AMPK its downstream target acetyl-CoA...
Metabolic aberrations impact the pathogenesis of multiple sclerosis (MS) and possibly can provide clues for new treatment strategies. Using untargeted metabolomics, we measured serum metabolites from 35 patients with relapsing-remitting (RRMS) 14 healthy age-matched controls. Of 632 known detected, 60 were significantly altered in RRMS. Bioinformatics analysis identified an metabotype RRMS, represented by four changed metabolic pathways glycerophospholipid, citrate cycle, sphingolipid,...
Summary Herpetic epithelial and stromal keratitis is a sight‐threatening ocular infection. To study the role of epithelium in innate response to herpes simplex virus 1 (HSV‐1) infection cornea, we used telomerase‐immortalized human corneal cell (HCEC) line, HUCL, primary HCECs as model infected cells with HSV‐1 (KOS strain). resulted two‐phase activation nuclear factor‐kappaB (NF‐κB), JNK p38, first peak at 1–4 hr second 8 hr. Concomitant activation, transcriptional expression interleukin...
Abstract TLRs are required for generation of protective lung mucosal immune responses against microbial pathogens. In this study, we evaluated the effect TLR5 ligand flagellin on stimulation antibacterial immunity in a lethal murine Pseudomonas aeruginosa pneumonia model. The intranasal pretreatment mice with purified P. induced strong protection intratracheal aeruginosa-induced lethality, which was attributable to markedly improved bacterial clearance, reduced dissemination, and decreased...
ABSTRACT The treatment of endophthalmitis is becoming very challenging due to the emergence multidrug-resistant bacteria. Hence, development novel therapeutic alternatives for ocular use essential. Here, we evaluated potential Ply187AN-KSH3b, a chimeric phage endolysin derived from Ply187 prophage, in mouse model Staphylococcus aureus endophthalmitis. Our data showed that exhibited strong antimicrobial activity against both methicillin-sensitive S. and methicillin-resistant (MRSA) strains,...
Ocular surgeries and trauma predispose the eye to develop infectious endophthalmitis, which often leads vision loss. The mechanisms of initiation innate defense in this disease are not well understood but presumed involve retinal glial cells. We hypothesize that Muller glia can recognize respond invading pathogens via TLRs, key regulators immune system. Using mouse sections, human cell line (MIO-M1), primary cells, we show they express known TLR1-10, adaptor molecules MyD88, TRIF, TRAM,...
ABSTRACT Staphylococcus aureus is a leading cause of severe endophthalmitis, which often results in vision loss some patients. Previously, we showed that Toll-like receptor 2 (TLR2) ligand pretreatment prevented the development staphylococcal endophthalmitis mice and suggested microglia might be involved this protective effect (Kumar A, Singh CN, Glybina IV, Mahmoud TH, Yu FS. J. Infect. Dis. 201:255–263, 2010). The aim present study was to understand how microglial innate response modulated...
Nuclear factor-κB (NF-κB), a key regulator of immune and inflammatory responses, plays important roles in diabetes-induced microvascular complications including diabetic retinopathy (DR). Thrombin activates NF-κB through protease-activated receptor (PAR)-1, member the G-protein-coupled (GPCR) superfamily, contributes to DR. The current study is uncover microRNA (miRNA) thrombin-induced activation retinal endothelial functions.Target prediction was performed using TargetScan algorithm....
The eye is highly susceptible to inflammation-mediated tissue damage evoked during bacterial infection. However, mechanisms regulating inflammation protect the remain elusive. Here, we used integrated metabolomics and transcriptomics show that immunomodulatory metabolite itaconate immune-responsive gene 1 (Irg1) are induced in (Staphylococcus aureus)-infected mouse eyes, bone-marrow-derived macrophages (BMDMs), Müller glia. Itaconate levels also elevated vitreous of patients with...
Abstract Muller cells, the principal glia of retina, play several key roles in normal and various retinal diseases. To date, their direct involvement innate defense against bacterial pathogens has not been investigated. In this article, we show that cells express TLR2, a sensor implicated recognizing Gram-positive bacteria. We found intravitreal injection TLR2 agonist Pam3Cys Staphylococcus aureus activated C57BL/6 mouse retina. Similarly, or S. elicited expression NF-κB p38 MAPK signaling...
Purpose.: Preclinical studies have highlighted retinal oxidative stress in the pathogenesis of diabetic retinopathy. We evaluated whether a treatment designed to enhance cellular catalase reduces cells cultured high glucose and mice corrects an imaging biomarker responsive antioxidant therapy (manganese-enhanced magnetic resonance [MEMRI]). Methods.: Human Müller pigment epithelial were chronically exposed normal or levels treated with cell-penetrating derivative peroxisomal enzyme (called...