A5102933471- Eicosanoids and Hypertension Pharmacology
- Inflammasome and immune disorders
- Inflammation biomarkers and pathways
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Neonatal Respiratory Health Research
- Heme Oxygenase-1 and Carbon Monoxide
- Respiratory Support and Mechanisms
- Alcohol Consumption and Health Effects
- Cell death mechanisms and regulation
- Immune Response and Inflammation
- Diet, Metabolism, and Disease
- Personality Disorders and Psychopathology
- Mitochondrial Function and Pathology
- Neuropeptides and Animal Physiology
- Pediatric health and respiratory diseases
- Sepsis Diagnosis and Treatment
- Pulmonary Hypertension Research and Treatments
- Cancer-related molecular mechanisms research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Autophagy in Disease and Therapy
- Advances in Cucurbitaceae Research
- earthquake and tectonic studies
- Geomagnetism and Paleomagnetism Studies
- Chromosomal and Genetic Variations
- Genomics, phytochemicals, and oxidative stress
Health & Life (Taiwan)
2025
Central South University
2012-2024
Heilongjiang University of Chinese Medicine
2024
Second Xiangya Hospital of Central South University
2018-2024
Institute of Geology and Geophysics
2016-2023
Chinese Academy of Sciences
2016-2023
Sabin Vaccine Institute
2023
University of Cincinnati Medical Center
2023
Henan Agricultural University
2019-2022
Ministry of Agriculture and Rural Affairs
2021
Studies of gene function in non-model animals have been limited by the approaches available for eliminating function. The CRISPR/Cas9 ( C: lustered R: egularly I: nterspaced S: hort P: alindromic epeats/ RISPR AS: sociated) system has recently become a powerful tool targeted genome editing. Here, we report use to disrupt selected genes, including nanos2, nanos3, dmrt1, and foxl2, with efficiencies as high 95%. In addition, mutations dmrt1 foxl2 induced were efficiently transmitted through...
Rationale: Dysregulation of arachidonic acid (ARA) metabolism results in inflammation; however, its role acute lung injury (ALI) remains elusive.In this study, we addressed the dysregulated ARA cytochromes P450 (CYPs) /cyclooxygenase-2 (COX-2) pathways pathogenesis lipopolysaccharide (LPS)-induced ALI mice.Methods: The CYPs/COX-2-derived lungs LPS-induced was investigated C57BL/6 mice.The COX-2/sEH dual inhibitor PTUPB used to establish function CYPs/COX-2 dysregulation ALI.Primary murine...
The triggering receptor expressed on myeloid cells-1 (TREM-1) is a pro-inflammatory immune potentiating acute lung injury (ALI).However, the mechanism of TREM-1-triggered inflammation response remains poorly understood.Here, we showed that TREM-1 blocking attenuated NOD-, LRRand pyrin domain-containing 3 (NLRP3) inflammasome activation and glycolysis in LPS-induced ALI mice.Then, observed enhanced glucose consumption, induced glycolysis, inhibited oxidative phosphorylation...
Transcription activator-like effector nucleases (TALENs) are a powerful approach for targeted genome editing and have been proved to be effective in several organisms. In this study, we reported that TALENs can induce somatic mutations Nile tilapia, an important species worldwide aquaculture, with reliably high efficiency. Six pairs of were constructed target genes related sex differentiation, including dmrt1, foxl2, cyp19a1a, gsdf, igf3, nrob1b, all resulted indel maximum efficiencies up...
Abstract Gluconic metabolic reprogramming, immune response, and inflammation are intimately linked. Glycolysis involves in the pathologic progress acute chronic inflammatory diseases. However, involvement of glycolysis lung injury (ALI) is still unclear. This study investigated role an animal model ALI. First, we found that lactate content serum was remarkably increased ALI patients a murine induced by intratracheal administration lipopolysaccharide (LPS). The key proteins involving were...
We previously found that the disorder of soluble epoxide hydrolase (sEH)/cyclooxygenase-2 (COX-2)-mediated arachidonic acid (ARA) metabolism contributes to pathogenesis non-alcoholic fatty liver disease (NAFLD) in mice. However, exact mechanism has not been elucidated. Accumulating evidence points essential role cellular senescence NAFLD. Herein, we investigated whether restoring balance sEH/COX-2-mediated ARA attenuated NAFLD via hepatocyte senescence. A promised dual inhibitor sEH and...
Abstract Necroptosis is the major cause of death in alveolar epithelial cells (AECs) during acute lung injury (ALI). Here, we report a previously unrecognized mechanism for necroptosis. We found an accumulation mitochondrial citrate (citrate mt ) lipopolysaccharide (LPS)-treated AECs because downregulation Idh3α and carrier (CIC, also known as Slc25a1). shRNA- or inhibitor–mediated inhibition Slc25a1 induced necroptosis vitro. Mice with AEC-specific deficiency exhibited exacerbated AEC...
Alveolar epithelial cell (AEC) senescence is a key driver of variety chronic lung diseases. It remains challenge how to alleviate AEC and mitigate disease progression. Our study identified critical role epoxyeicosatrienoic acids (EETs), downstream metabolites arachidonic acid (ARA) by cytochrome p450 (CYP), in alleviating senescence. In vitro, we found that 14,15-EET content was significantly decreased senescent AECs. Exogenous EETs supplementation, overexpression CYP2J2, or inhibition...
Abstract Background Necroptosis of macrophages is a necessary element in reinforcing intrapulmonary inflammation during acute lung injury (ALI). However, the molecular mechanism that sparks macrophage necroptosis still unclear. Triggering receptor expressed on myeloid cells-1 (TREM-1) pattern recognition broadly monocytes/macrophages. The influence TREM-1 destiny ALI requires further investigation. Methods decoy LR12 was used to evaluate whether activation induced lipopolysaccharide...
Schizophrenia is a disease with complex pathological mechanism that influenced by multiple genes. The study of its pathogenesis dominated the dopamine hypothesis, as well other hypotheses such 5-hydroxytryptamine glutamate immune-inflammatory gene expression abnormality and neurodevelopmental hypothesis. first generation antipsychotics was developed based on dopaminergic receptor antagonism, which blocks D2 receptors in brain to exert antipsychotic effects. second acts dual blockade...
Lung cancer is the most frequent type and leading cause of tumor-associated deaths worldwide.TP53 an important tumor suppressor gene frequently inactivated in lung cancer.E3 ligases targeting p53, such as MDM2, are involved development cancer.The E3 ligase HUWE1, which targets many proteins including has been reported to be highly expressed cancer; however, its role tumorigenesis unclear.Methods: The expression HUWE1 p53 cells was modulated phenotypes were assessed by performing soft agar...
Pulmonary fibrosis (PF) is a senescence-associated disease with poor prognosis. Currently, there no effective therapeutic strategy for preventing and treating the process. Mounting evidence suggests that arachidonic acid (ARA) metabolites are involved in pathogenesis of various fibrosis. However, relationship between metabolism ARA PF still elusive. In this study, we observed disorder cyclooxygenase-2/cytochrome P450 (COX-2/CYP) lungs mice induced by bleomycin (BLM). Therefore, aimed to...
Arachidonic acid can be metabolized to prostaglandins and epoxyeicosatrienoic acids (EETs) by cyclooxygenase-2 (COX-2) cytochrome P450 (CYP), respectively. While protective EETs are degraded soluble epoxide hydrolase (sEH) very fast. We have reported that dual inhibition of COX-2 sEH with specific inhibitor PTUPB shows anti-pulmonary fibrosis renal protection. However, the effect on cecal ligation puncture (CLP)-induced sepsis remains unclear. The current study aimed investigate effects...
Abstract Necroptosis, a recently described form of programmed cell death, is the main way alveolar epithelial cells (AECs) death in acute lung injury (ALI). While mechanism how to trigger necroptosis AECs during ALI has been rarely evaluated. Long optic atrophy protein 1 (L‐OPA1) crucial mitochondrial inner membrane fusion protein, and its deficiency impairs function. This study aimed investigate role L‐OPA1 deficiency‐mediated dysfunction necroptosis. We comprehensively investigated...
Abstract Background The epithelial-mesenchymal transition (EMT) of human bronchial epithelial cells (HBECs) is essential for airway remodeling during asthma. Wnt5a has been implicated in various lung diseases, while its role the EMT HBECs asthma yet to be determined. This study sought define whether initiated EMT, leading through induction autophagy HBECs. Methods Microarray analysis was used investigate expression change WNT5A patients. In parallel, models were induced using 16HBE by...
A visible‐light‐driven E‐selective semihydrogenation scheme of alkyne compounds was developed for designing and synthesizing glioblastoma multiforme (GBM) inhibitors. In the present study, room‐temperature reactions various were successfully achieved under irradiation white light. The representative reaction mechanism decoded through theoretical calculations deuteration experiments, revealing critical roles played by [Ir(Cp‐Me5)Cl2]2, PdCl2, DPPE TEOA as photosensitizer, catalyst, ligand,...
Vasoactive intestinal peptide (VIP) is a neuropeptide that exerts anti-inflammatory functions. We have reported VIP mediated by lentivirus attenuates acute lung injury (ALI) in lipopolysaccharide (LPS)-induced murine model. However, the exact role of uncontrolled inflammation during ALI largely unknown. Accumulating evidence indicates NLRP3 inflammasome has critical ALI. In this study, we investigated effects on activation development mice. Seven days after intratracheal injection...
Accumulating evidence indicates that angiotensin (1-7) [Ang-(1-7)] protects against idiopathic pulmonary fibrosis (IPF) in animal experiments. However, whether Ang-(1-7) effectively inhibits epithelial-mesenchymal transition (EMT) induced by transforming growth factor-β1 (TGF-β1) remains unclear. The aim of this study is to examine the eff ;ects on TGF-β1-induced EMT human alveolar epithelial cells. We found angiotensin-converting enzyme 2 (ACE2) /Ang-(1-7)/MasR were decreased lungs mice with...
Epoxyeicosatrienoic acids (EETs) derived from arachidonic acid exert anti-inflammation effects. We have reported that blocking the degradation of EETs with a soluble epoxide hydrolase (sEH) inhibitor protects mice lipopolysaccharide (LPS)-induced acute lung injury (ALI). The underlying mechanisms remain essential questions. In this study, we investigated effects on activation nucleotide-binding domain leucine-rich repeat-containing receptor, pyrin domain-containing-3 (NLRP3) inflammasome in...